Increased Frequency of Copy Number Variations Revealed by Array Comparative Genomic Hybridization in the Offspring of Male Mice Exposed to Low Dose-Rate Ionizing Radiation
There is very little information on the transgenerational or genetic effects of low dose-rate ionizing radiation. We report the detection of the transgenerational effects of chronic low dose-rate irradiation in mice, at the molecular level in the whole genome, using array comparative genomic hybridi...
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MDPI AG
2021-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/22/12437 |
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| author | Keiji Ogura Yoshiko Ayabe Chihiro Harada Ignacia Braga Tanaka Satoshi Tanaka Jun-ichiro Komura |
| author_facet | Keiji Ogura Yoshiko Ayabe Chihiro Harada Ignacia Braga Tanaka Satoshi Tanaka Jun-ichiro Komura |
| author_sort | Keiji Ogura |
| collection | DOAJ |
| description | There is very little information on the transgenerational or genetic effects of low dose-rate ionizing radiation. We report the detection of the transgenerational effects of chronic low dose-rate irradiation in mice, at the molecular level in the whole genome, using array comparative genomic hybridization technology. We observed that the number of the mice with de novo copy number variations (specifically, deletions) was significantly increased in the offspring of C57BL/6J male mice exposed to 20 mGy/day gamma-rays for 400 days (total dose: 8000 mGy), as compared to non-irradiated controls. We did not detect any difference in the size of the de novo deletions between the irradiated and the non-irradiated groups. An analysis of the life span of the offspring suggested a possibility that de novo copy-number variations may be associated with shorter life spans. |
| first_indexed | 2024-03-10T05:25:45Z |
| format | Article |
| id | doaj.art-87cde449a2ba4eb7b38b9026b6cdc156 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T05:25:45Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-87cde449a2ba4eb7b38b9026b6cdc1562023-11-22T23:42:21ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-11-0122221243710.3390/ijms222212437Increased Frequency of Copy Number Variations Revealed by Array Comparative Genomic Hybridization in the Offspring of Male Mice Exposed to Low Dose-Rate Ionizing RadiationKeiji Ogura0Yoshiko Ayabe1Chihiro Harada2Ignacia Braga Tanaka3Satoshi Tanaka4Jun-ichiro Komura5Department of Radiobiology, Institute for Environmental Sciences, Aomori 039-3213, JapanDepartment of Radiobiology, Institute for Environmental Sciences, Aomori 039-3213, JapanJAC Co., Ltd., Tokyo 101-0051, JapanDepartment of Radiobiology, Institute for Environmental Sciences, Aomori 039-3213, JapanDepartment of Radiobiology, Institute for Environmental Sciences, Aomori 039-3213, JapanDepartment of Radiobiology, Institute for Environmental Sciences, Aomori 039-3213, JapanThere is very little information on the transgenerational or genetic effects of low dose-rate ionizing radiation. We report the detection of the transgenerational effects of chronic low dose-rate irradiation in mice, at the molecular level in the whole genome, using array comparative genomic hybridization technology. We observed that the number of the mice with de novo copy number variations (specifically, deletions) was significantly increased in the offspring of C57BL/6J male mice exposed to 20 mGy/day gamma-rays for 400 days (total dose: 8000 mGy), as compared to non-irradiated controls. We did not detect any difference in the size of the de novo deletions between the irradiated and the non-irradiated groups. An analysis of the life span of the offspring suggested a possibility that de novo copy-number variations may be associated with shorter life spans.https://www.mdpi.com/1422-0067/22/22/12437ionizing radiationlow dose-ratetransgenerational effectsgenetic effectscopy number variation (CNV)array comprehensive genomic hybridization (array CGH) |
| spellingShingle | Keiji Ogura Yoshiko Ayabe Chihiro Harada Ignacia Braga Tanaka Satoshi Tanaka Jun-ichiro Komura Increased Frequency of Copy Number Variations Revealed by Array Comparative Genomic Hybridization in the Offspring of Male Mice Exposed to Low Dose-Rate Ionizing Radiation International Journal of Molecular Sciences ionizing radiation low dose-rate transgenerational effects genetic effects copy number variation (CNV) array comprehensive genomic hybridization (array CGH) |
| title | Increased Frequency of Copy Number Variations Revealed by Array Comparative Genomic Hybridization in the Offspring of Male Mice Exposed to Low Dose-Rate Ionizing Radiation |
| title_full | Increased Frequency of Copy Number Variations Revealed by Array Comparative Genomic Hybridization in the Offspring of Male Mice Exposed to Low Dose-Rate Ionizing Radiation |
| title_fullStr | Increased Frequency of Copy Number Variations Revealed by Array Comparative Genomic Hybridization in the Offspring of Male Mice Exposed to Low Dose-Rate Ionizing Radiation |
| title_full_unstemmed | Increased Frequency of Copy Number Variations Revealed by Array Comparative Genomic Hybridization in the Offspring of Male Mice Exposed to Low Dose-Rate Ionizing Radiation |
| title_short | Increased Frequency of Copy Number Variations Revealed by Array Comparative Genomic Hybridization in the Offspring of Male Mice Exposed to Low Dose-Rate Ionizing Radiation |
| title_sort | increased frequency of copy number variations revealed by array comparative genomic hybridization in the offspring of male mice exposed to low dose rate ionizing radiation |
| topic | ionizing radiation low dose-rate transgenerational effects genetic effects copy number variation (CNV) array comprehensive genomic hybridization (array CGH) |
| url | https://www.mdpi.com/1422-0067/22/22/12437 |
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