Serotype distribution and antibiotic resistance of Streptococcus pneumoniae isolates collected from unvaccinated children with pneumonia at a province in central Vietnam
Background and Objectives: Identification of pnemococcal serotypes and antimicrobial resistance provides helpful information for the use of suitable vaccines and antibiotics; however, very limited data is available on these issues in Vietnam. The present study aimed to find the serotype distributio...
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| Format: | Article |
| Idioma: | English |
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Tehran University of Medical Sciences
2022-10-01
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| Col·lecció: | Iranian Journal of Microbiology |
| Matèries: | |
| Accés en línia: | https://ijm.tums.ac.ir/index.php/ijm/article/view/3745 |
| _version_ | 1828218801129259008 |
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| author | Bui Anh Son Tang Xuan Hai Tran Van Cuong Duong Dinh Chinh Thi-Hong-Hanh Le Nguyen Manh Dung Vu Nhat Dinh Do Ngoc Anh |
| author_facet | Bui Anh Son Tang Xuan Hai Tran Van Cuong Duong Dinh Chinh Thi-Hong-Hanh Le Nguyen Manh Dung Vu Nhat Dinh Do Ngoc Anh |
| author_sort | Bui Anh Son |
| collection | DOAJ |
| description |
Background and Objectives: Identification of pnemococcal serotypes and antimicrobial resistance provides helpful information for the use of suitable vaccines and antibiotics; however, very limited data is available on these issues in Vietnam. The present study aimed to find the serotype distribution and drug resistance patterns of Streptococcus pneumoniae isolated from unvaccinated children less than 5 years of age with pneumonia at a province in centre Vietnam.
Materials and Methods: A total of 126 clinical pnemococcal strains isolated from unvaccinated children less than 5 years of age with pneumonia at the Nghe An province, Vietnam between Nov 2019 and Mar 2021. All strains were identified using conventional microbiological method, VITEK® 2 Compact system, specific PCR and sequencing. The serotypes and antimicrobial resistance patterns of pnemococcal strains were determined using the multiplex PCR assays and VITEK® 2 Compact system.
Results: The results showed that, eight different pneumococcal serotypes were identified. The most common serotypes were 19F (67.46%), followed by 23F (10.32%), 19A (9.52%), 6A/B (3.17%), 15A (2.38%), 9V (3.17%), 11A (1.59%) and 14 (0.80%), respectively. More than half of the pneumococcal strains were non-susceptible to penicillin. The resistance rate to ceftriaxone and cefotaxime were 41.3% and 50.8%. The percentage of pneumococci strains resistant to clarithromycin, azithromycin, erythromycin, cotrimoxazole, tetracyclin, and clindamycin were more than 93% of all strains. All pneumococcal serotypes were highly resistant to clarithromycin, azithromycin, erythromycin, cotrimoxazole, and clindamycin.
Conclusion: Our findings showed high antibiotic resistance rates of the strains causing pneumococcal pneumonia, mostly macrolide resistance, among unvaccinated children.
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| first_indexed | 2024-04-12T16:08:11Z |
| format | Article |
| id | doaj.art-87d0b994b51f48a6bb71b43e4a4f2dbb |
| institution | Directory Open Access Journal |
| issn | 2008-3289 2008-4447 |
| language | English |
| last_indexed | 2024-04-12T16:08:11Z |
| publishDate | 2022-10-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj.art-87d0b994b51f48a6bb71b43e4a4f2dbb2022-12-22T03:25:59ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472022-10-01145Serotype distribution and antibiotic resistance of Streptococcus pneumoniae isolates collected from unvaccinated children with pneumonia at a province in central VietnamBui Anh Son0Tang Xuan Hai1Tran Van Cuong2Duong Dinh Chinh3Thi-Hong-Hanh Le4Nguyen Manh Dung5Vu Nhat Dinh6Do Ngoc Anh7Department of Pediatrics, Nghe An Obstetrics and Pediatrics Hospital, Nghe An, VietnamDepartment of Pediatrics, Nghe An Obstetrics and Pediatrics Hospital, Nghe An, VietnamDepartment of Pediatrics, Nghe An Obstetrics and Pediatrics Hospital, Nghe An, VietnamDepartment of Neurology, Nghe An Friendship General Hospital, Nghe An, VietnamDepartment of Respiratory Diseases, National Pediatrics Hospital, Hanoi, VietnamDepartment of Scientific Management, 108 Military Centre Hospital, Hanoi, VietnamDepartment of Trauma and Orthopedic Surgery, 103 Military Hospital, Hanoi, Vietnam; Department of Laboratory Medicine, 103 Military Hospital, Hanoi, VietnamDepartment of Laboratory Medicine, 103 Military Hospital, Hanoi, Vietnam; Department of Medical Parasitology, Vietnam Military Medical University, Hanoi, Vietnam Background and Objectives: Identification of pnemococcal serotypes and antimicrobial resistance provides helpful information for the use of suitable vaccines and antibiotics; however, very limited data is available on these issues in Vietnam. The present study aimed to find the serotype distribution and drug resistance patterns of Streptococcus pneumoniae isolated from unvaccinated children less than 5 years of age with pneumonia at a province in centre Vietnam. Materials and Methods: A total of 126 clinical pnemococcal strains isolated from unvaccinated children less than 5 years of age with pneumonia at the Nghe An province, Vietnam between Nov 2019 and Mar 2021. All strains were identified using conventional microbiological method, VITEK® 2 Compact system, specific PCR and sequencing. The serotypes and antimicrobial resistance patterns of pnemococcal strains were determined using the multiplex PCR assays and VITEK® 2 Compact system. Results: The results showed that, eight different pneumococcal serotypes were identified. The most common serotypes were 19F (67.46%), followed by 23F (10.32%), 19A (9.52%), 6A/B (3.17%), 15A (2.38%), 9V (3.17%), 11A (1.59%) and 14 (0.80%), respectively. More than half of the pneumococcal strains were non-susceptible to penicillin. The resistance rate to ceftriaxone and cefotaxime were 41.3% and 50.8%. The percentage of pneumococci strains resistant to clarithromycin, azithromycin, erythromycin, cotrimoxazole, tetracyclin, and clindamycin were more than 93% of all strains. All pneumococcal serotypes were highly resistant to clarithromycin, azithromycin, erythromycin, cotrimoxazole, and clindamycin. Conclusion: Our findings showed high antibiotic resistance rates of the strains causing pneumococcal pneumonia, mostly macrolide resistance, among unvaccinated children. https://ijm.tums.ac.ir/index.php/ijm/article/view/3745Streptococcus pneumoniae;Serotypes;Antibiotic resistance;Children;Pneumonia |
| spellingShingle | Bui Anh Son Tang Xuan Hai Tran Van Cuong Duong Dinh Chinh Thi-Hong-Hanh Le Nguyen Manh Dung Vu Nhat Dinh Do Ngoc Anh Serotype distribution and antibiotic resistance of Streptococcus pneumoniae isolates collected from unvaccinated children with pneumonia at a province in central Vietnam Iranian Journal of Microbiology Streptococcus pneumoniae; Serotypes; Antibiotic resistance; Children; Pneumonia |
| title | Serotype distribution and antibiotic resistance of Streptococcus pneumoniae isolates collected from unvaccinated children with pneumonia at a province in central Vietnam |
| title_full | Serotype distribution and antibiotic resistance of Streptococcus pneumoniae isolates collected from unvaccinated children with pneumonia at a province in central Vietnam |
| title_fullStr | Serotype distribution and antibiotic resistance of Streptococcus pneumoniae isolates collected from unvaccinated children with pneumonia at a province in central Vietnam |
| title_full_unstemmed | Serotype distribution and antibiotic resistance of Streptococcus pneumoniae isolates collected from unvaccinated children with pneumonia at a province in central Vietnam |
| title_short | Serotype distribution and antibiotic resistance of Streptococcus pneumoniae isolates collected from unvaccinated children with pneumonia at a province in central Vietnam |
| title_sort | serotype distribution and antibiotic resistance of streptococcus pneumoniae isolates collected from unvaccinated children with pneumonia at a province in central vietnam |
| topic | Streptococcus pneumoniae; Serotypes; Antibiotic resistance; Children; Pneumonia |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/3745 |
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