Anticancer activities of phenolic compounds from Moringa oleifera leaves: in vitro and in silico mechanistic study
Abstract Background Moringa oleifera is a common vegetable in many countries since ancient times, possesses numerous phenolic compounds having a wide array of biological activities. It possesses anticancer activity that can be used to develop new drugs for treatment of various types of cancers. The...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2021-02-01
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| Series: | Beni-Suef University Journal of Basic and Applied Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s43088-021-00101-2 |
| _version_ | 1818957764556226560 |
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| author | Muhammad Zahid Mumtaz Fareeda Kausar Mubashir Hassan Shaista Javaid Arif Malik |
| author_facet | Muhammad Zahid Mumtaz Fareeda Kausar Mubashir Hassan Shaista Javaid Arif Malik |
| author_sort | Muhammad Zahid Mumtaz |
| collection | DOAJ |
| description | Abstract Background Moringa oleifera is a common vegetable in many countries since ancient times, possesses numerous phenolic compounds having a wide array of biological activities. It possesses anticancer activity that can be used to develop new drugs for treatment of various types of cancers. The current study was conducted to evaluate the composition of phenolic compounds and in vitro and in silico anticancer activities of M. oleifera leaves extracts. The leaves of M. oleifera were subjected to extraction for solvent fraction using n-hexane, chloroform, ethyl acetate, butanol, and aqueous solvents. The solvent fractions were tested for anticancer activity in vitro against Hela cancer cell line and screened for phenolic compounds through reversed-phase high-performance liquid chromatography. The molecular docking approach was employed to check binding conformations of phytochemicals against the target protein. Result The result revealed that all the solvent fractions possess in vitro anticancer activity against Hela cancer cell line. The n-hexane fraction showed a 50% reduction in Hela cancer cell viability at 416 μg mL−1 as compared to control. The extracts of solvent-fraction contained 10 phenolic compounds viz. quercetin, gallic acid, sinapic acid, vanillic acid, 4-hydroxy-3-methoxy benzoic acid, p-coumaric acid, m-coumaric acid, 4-hydroxy-3-methoxy cinnamic acid, caffeic acid, and syringic acid. Molecular docking studies revealed that the ligands bind within the active site of target protein have good binding energy values. Conclusion This study shows that M. oleifera leaves may have the potential to inhibit cancer cell growth and improving human health in addition to food ingredient innovations. Based on in vitro and in silico results, the phytochemicals from M. oleifera leaves can be used as leading drugs to treat cancer. Graphical abstract |
| first_indexed | 2024-12-20T11:15:02Z |
| format | Article |
| id | doaj.art-87d2ba84de99414a9a711ba7573c6ecd |
| institution | Directory Open Access Journal |
| issn | 2314-8543 |
| language | English |
| last_indexed | 2024-12-20T11:15:02Z |
| publishDate | 2021-02-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Beni-Suef University Journal of Basic and Applied Sciences |
| spelling | doaj.art-87d2ba84de99414a9a711ba7573c6ecd2022-12-21T19:42:39ZengSpringerOpenBeni-Suef University Journal of Basic and Applied Sciences2314-85432021-02-0110111110.1186/s43088-021-00101-2Anticancer activities of phenolic compounds from Moringa oleifera leaves: in vitro and in silico mechanistic studyMuhammad Zahid Mumtaz0Fareeda Kausar1Mubashir Hassan2Shaista Javaid3Arif Malik4Institute of Molecular Biology and Biotechnology, The University of LahoreInstitute of Molecular Biology and Biotechnology, The University of LahoreInstitute of Molecular Biology and Biotechnology, The University of LahoreInstitute of Molecular Biology and Biotechnology, The University of LahoreInstitute of Molecular Biology and Biotechnology, The University of LahoreAbstract Background Moringa oleifera is a common vegetable in many countries since ancient times, possesses numerous phenolic compounds having a wide array of biological activities. It possesses anticancer activity that can be used to develop new drugs for treatment of various types of cancers. The current study was conducted to evaluate the composition of phenolic compounds and in vitro and in silico anticancer activities of M. oleifera leaves extracts. The leaves of M. oleifera were subjected to extraction for solvent fraction using n-hexane, chloroform, ethyl acetate, butanol, and aqueous solvents. The solvent fractions were tested for anticancer activity in vitro against Hela cancer cell line and screened for phenolic compounds through reversed-phase high-performance liquid chromatography. The molecular docking approach was employed to check binding conformations of phytochemicals against the target protein. Result The result revealed that all the solvent fractions possess in vitro anticancer activity against Hela cancer cell line. The n-hexane fraction showed a 50% reduction in Hela cancer cell viability at 416 μg mL−1 as compared to control. The extracts of solvent-fraction contained 10 phenolic compounds viz. quercetin, gallic acid, sinapic acid, vanillic acid, 4-hydroxy-3-methoxy benzoic acid, p-coumaric acid, m-coumaric acid, 4-hydroxy-3-methoxy cinnamic acid, caffeic acid, and syringic acid. Molecular docking studies revealed that the ligands bind within the active site of target protein have good binding energy values. Conclusion This study shows that M. oleifera leaves may have the potential to inhibit cancer cell growth and improving human health in addition to food ingredient innovations. Based on in vitro and in silico results, the phytochemicals from M. oleifera leaves can be used as leading drugs to treat cancer. Graphical abstracthttps://doi.org/10.1186/s43088-021-00101-2Anticancer activityHela cancer lineMolecular dockingMoringa oleiferaPhenolic acidsSolvent fractions |
| spellingShingle | Muhammad Zahid Mumtaz Fareeda Kausar Mubashir Hassan Shaista Javaid Arif Malik Anticancer activities of phenolic compounds from Moringa oleifera leaves: in vitro and in silico mechanistic study Beni-Suef University Journal of Basic and Applied Sciences Anticancer activity Hela cancer line Molecular docking Moringa oleifera Phenolic acids Solvent fractions |
| title | Anticancer activities of phenolic compounds from Moringa oleifera leaves: in vitro and in silico mechanistic study |
| title_full | Anticancer activities of phenolic compounds from Moringa oleifera leaves: in vitro and in silico mechanistic study |
| title_fullStr | Anticancer activities of phenolic compounds from Moringa oleifera leaves: in vitro and in silico mechanistic study |
| title_full_unstemmed | Anticancer activities of phenolic compounds from Moringa oleifera leaves: in vitro and in silico mechanistic study |
| title_short | Anticancer activities of phenolic compounds from Moringa oleifera leaves: in vitro and in silico mechanistic study |
| title_sort | anticancer activities of phenolic compounds from moringa oleifera leaves in vitro and in silico mechanistic study |
| topic | Anticancer activity Hela cancer line Molecular docking Moringa oleifera Phenolic acids Solvent fractions |
| url | https://doi.org/10.1186/s43088-021-00101-2 |
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