Expression profiling of chemokines CCL8 and CXCL10 during peri-implantation period in Murrah buffaloes as a possible signature of embryonic implantation
The present investigation was aimed to evaluate novel implication of chemokine genes CXCL10 (C-X-C motif chemokine 10) and CCL 8 (C-C motif chemokine 8) genes for use as ideal pregnancy biomarker in dairy buffaloes. We studied expression profile of both these chemokine genes in whole blood of Murrah...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Indian Council of Agricultural Research
2021-09-01
|
| Series: | Indian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | https://epubs.icar.org.in/index.php/IJAnS/article/view/115920 |
| _version_ | 1828006033198415872 |
|---|---|
| author | RAJNI KUMARI S DAYAL I SHARMA S KUMAR P C CHANDRAN A DEY P K RAY R K KAMAL JYOTI KUMAR KAMAL SARMA |
| author_facet | RAJNI KUMARI S DAYAL I SHARMA S KUMAR P C CHANDRAN A DEY P K RAY R K KAMAL JYOTI KUMAR KAMAL SARMA |
| author_sort | RAJNI KUMARI |
| collection | DOAJ |
| description | The present investigation was aimed to evaluate novel implication of chemokine genes CXCL10 (C-X-C motif chemokine 10) and CCL 8 (C-C motif chemokine 8) genes for use as ideal pregnancy biomarker in dairy buffaloes. We studied expression profile of both these chemokine genes in whole blood of Murrah buffaloes on day 12, 15, 18 and 21 post artificial insemination (AI) using SYBR green chemistry based quantitative real time reverse transcription PCR. Our investigations revealed a consistent increase in transcriptional abundance of CCL8 and CXCL10 genes during this period, although the difference in expression level was not significant between day 15 and 18 post AI for CXCL10 gene. We also studied the effect of parity on the expression profile of these two genes and found that expression level of both these genes is independent of parity status of the animal. Based on the results, it can be concluded that these chemokine genes can be used as early pregnancy biomarker on any day between days 12 to 21 post artificial insemination in buffaloes irrespective of their parity status. |
| first_indexed | 2024-04-10T07:51:05Z |
| format | Article |
| id | doaj.art-87d8b49fc95146a688cf7b2f682c4a65 |
| institution | Directory Open Access Journal |
| issn | 0367-8318 2394-3327 |
| language | English |
| last_indexed | 2024-04-10T07:51:05Z |
| publishDate | 2021-09-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | Indian Journal of Animal Sciences |
| spelling | doaj.art-87d8b49fc95146a688cf7b2f682c4a652023-02-23T10:12:03ZengIndian Council of Agricultural ResearchIndian Journal of Animal Sciences0367-83182394-33272021-09-0191810.56093/ijans.v91i8.115920Expression profiling of chemokines CCL8 and CXCL10 during peri-implantation period in Murrah buffaloes as a possible signature of embryonic implantationRAJNI KUMARI0S DAYAL1I SHARMA2S KUMAR3P C CHANDRAN4A DEY5P K RAY6R K KAMAL7JYOTI KUMAR8KAMAL SARMA9ICAR-Research Complex for Eastern Region, Patna, Bihar 800 014 IndiaICAR-Research Complex for Eastern Region, Patna, Bihar 800 014 IndiaSam Higginbottom University of Agriculture, Technology and Sciences, Allahabad, Uttar PradeshBihar Veterinary College, Patna, BiharICAR-Research Complex for Eastern Region, Patna, Bihar 800 014 IndiaICAR-Research Complex for Eastern Region, Patna, Bihar 800 014 IndiaICAR-Research Complex for Eastern Region, Patna, Bihar 800 014 IndiaICAR-Research Complex for Eastern Region, Patna, Bihar 800 014 IndiaICAR-Research Complex for Eastern Region, Patna, Bihar 800 014 IndiaICAR-Research Complex for Eastern Region, Patna, Bihar 800 014 IndiaThe present investigation was aimed to evaluate novel implication of chemokine genes CXCL10 (C-X-C motif chemokine 10) and CCL 8 (C-C motif chemokine 8) genes for use as ideal pregnancy biomarker in dairy buffaloes. We studied expression profile of both these chemokine genes in whole blood of Murrah buffaloes on day 12, 15, 18 and 21 post artificial insemination (AI) using SYBR green chemistry based quantitative real time reverse transcription PCR. Our investigations revealed a consistent increase in transcriptional abundance of CCL8 and CXCL10 genes during this period, although the difference in expression level was not significant between day 15 and 18 post AI for CXCL10 gene. We also studied the effect of parity on the expression profile of these two genes and found that expression level of both these genes is independent of parity status of the animal. Based on the results, it can be concluded that these chemokine genes can be used as early pregnancy biomarker on any day between days 12 to 21 post artificial insemination in buffaloes irrespective of their parity status.https://epubs.icar.org.in/index.php/IJAnS/article/view/115920BuffaloChemokine genesParity statusPregnancy biomarkerWhole blood sample |
| spellingShingle | RAJNI KUMARI S DAYAL I SHARMA S KUMAR P C CHANDRAN A DEY P K RAY R K KAMAL JYOTI KUMAR KAMAL SARMA Expression profiling of chemokines CCL8 and CXCL10 during peri-implantation period in Murrah buffaloes as a possible signature of embryonic implantation Indian Journal of Animal Sciences Buffalo Chemokine genes Parity status Pregnancy biomarker Whole blood sample |
| title | Expression profiling of chemokines CCL8 and CXCL10 during peri-implantation period in Murrah buffaloes as a possible signature of embryonic implantation |
| title_full | Expression profiling of chemokines CCL8 and CXCL10 during peri-implantation period in Murrah buffaloes as a possible signature of embryonic implantation |
| title_fullStr | Expression profiling of chemokines CCL8 and CXCL10 during peri-implantation period in Murrah buffaloes as a possible signature of embryonic implantation |
| title_full_unstemmed | Expression profiling of chemokines CCL8 and CXCL10 during peri-implantation period in Murrah buffaloes as a possible signature of embryonic implantation |
| title_short | Expression profiling of chemokines CCL8 and CXCL10 during peri-implantation period in Murrah buffaloes as a possible signature of embryonic implantation |
| title_sort | expression profiling of chemokines ccl8 and cxcl10 during peri implantation period in murrah buffaloes as a possible signature of embryonic implantation |
| topic | Buffalo Chemokine genes Parity status Pregnancy biomarker Whole blood sample |
| url | https://epubs.icar.org.in/index.php/IJAnS/article/view/115920 |
| work_keys_str_mv | AT rajnikumari expressionprofilingofchemokinesccl8andcxcl10duringperiimplantationperiodinmurrahbuffaloesasapossiblesignatureofembryonicimplantation AT sdayal expressionprofilingofchemokinesccl8andcxcl10duringperiimplantationperiodinmurrahbuffaloesasapossiblesignatureofembryonicimplantation AT isharma expressionprofilingofchemokinesccl8andcxcl10duringperiimplantationperiodinmurrahbuffaloesasapossiblesignatureofembryonicimplantation AT skumar expressionprofilingofchemokinesccl8andcxcl10duringperiimplantationperiodinmurrahbuffaloesasapossiblesignatureofembryonicimplantation AT pcchandran expressionprofilingofchemokinesccl8andcxcl10duringperiimplantationperiodinmurrahbuffaloesasapossiblesignatureofembryonicimplantation AT adey expressionprofilingofchemokinesccl8andcxcl10duringperiimplantationperiodinmurrahbuffaloesasapossiblesignatureofembryonicimplantation AT pkray expressionprofilingofchemokinesccl8andcxcl10duringperiimplantationperiodinmurrahbuffaloesasapossiblesignatureofembryonicimplantation AT rkkamal expressionprofilingofchemokinesccl8andcxcl10duringperiimplantationperiodinmurrahbuffaloesasapossiblesignatureofembryonicimplantation AT jyotikumar expressionprofilingofchemokinesccl8andcxcl10duringperiimplantationperiodinmurrahbuffaloesasapossiblesignatureofembryonicimplantation AT kamalsarma expressionprofilingofchemokinesccl8andcxcl10duringperiimplantationperiodinmurrahbuffaloesasapossiblesignatureofembryonicimplantation |