In Vitro Evaluation of Five Newly Isolated Bacteriophages against <i>E. faecalis</i> Biofilm for Their Potential Use against Post-Treatment Apical Periodontitis
State-of-the-art treatment of root canal infection includes the use of mechanical debridement and chemical agents. This disinfection method is limited, and microorganisms can remain in the canal system. <i>Enterococcus faecalis</i> appears with a high prevalence in secondary and persiste...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/14/9/1779 |
| _version_ | 1827657831485014016 |
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| author | Marie Voit Andrej Trampuz Mercedes Gonzalez Moreno |
| author_facet | Marie Voit Andrej Trampuz Mercedes Gonzalez Moreno |
| author_sort | Marie Voit |
| collection | DOAJ |
| description | State-of-the-art treatment of root canal infection includes the use of mechanical debridement and chemical agents. This disinfection method is limited, and microorganisms can remain in the canal system. <i>Enterococcus faecalis</i> appears with a high prevalence in secondary and persistent root canal infections and can be linked to endodontic treatment failure due to its various resistance mechanisms. Here, we evaluated the activity of newly isolated bacteriophages against clinical isolates of <i>E. faecalis</i> (including one vancomycin- and gentamicin-resistant strain) as a single treatment or in combination with gentamicin and vancomycin. For the resistant strain, daptomycin and fosfomycin were tested. Sixteen <i>E. faecalis</i> strains were used to screen for the presence of bacteriophages in sewage. Five different bacteriophages were characterized in terms of virion morphology, host range and killing-kinetics against each <i>E. faecalis</i> host strain. To investigate the antibiofilm effect of antibiotic and phages, <i>E. faecalis</i> biofilm was grown on porous glass beads and treated with different antibiotic concentrations and with isolated bacteriophages alone or in staggered combinations. A strong biofilm reduction was observed when phages were combined with antibiotic, where combinations with gentamicin showed a better outcome compared to vancomycin. Regarding the resistant strain, daptomycin had a superior antibiofilm effect than fosfomycin. |
| first_indexed | 2024-03-09T22:51:27Z |
| format | Article |
| id | doaj.art-87d96f143afa445abd7c621ad46f4376 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-09T22:51:27Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-87d96f143afa445abd7c621ad46f43762023-11-23T18:20:22ZengMDPI AGPharmaceutics1999-49232022-08-01149177910.3390/pharmaceutics14091779In Vitro Evaluation of Five Newly Isolated Bacteriophages against <i>E. faecalis</i> Biofilm for Their Potential Use against Post-Treatment Apical PeriodontitisMarie Voit0Andrej Trampuz1Mercedes Gonzalez Moreno2Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, Centre for Musculoskeletal Surgery, Augustenburger Platz 1, 13353 Berlin, GermanyCharité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, Centre for Musculoskeletal Surgery, Augustenburger Platz 1, 13353 Berlin, GermanyCharité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, Centre for Musculoskeletal Surgery, Augustenburger Platz 1, 13353 Berlin, GermanyState-of-the-art treatment of root canal infection includes the use of mechanical debridement and chemical agents. This disinfection method is limited, and microorganisms can remain in the canal system. <i>Enterococcus faecalis</i> appears with a high prevalence in secondary and persistent root canal infections and can be linked to endodontic treatment failure due to its various resistance mechanisms. Here, we evaluated the activity of newly isolated bacteriophages against clinical isolates of <i>E. faecalis</i> (including one vancomycin- and gentamicin-resistant strain) as a single treatment or in combination with gentamicin and vancomycin. For the resistant strain, daptomycin and fosfomycin were tested. Sixteen <i>E. faecalis</i> strains were used to screen for the presence of bacteriophages in sewage. Five different bacteriophages were characterized in terms of virion morphology, host range and killing-kinetics against each <i>E. faecalis</i> host strain. To investigate the antibiofilm effect of antibiotic and phages, <i>E. faecalis</i> biofilm was grown on porous glass beads and treated with different antibiotic concentrations and with isolated bacteriophages alone or in staggered combinations. A strong biofilm reduction was observed when phages were combined with antibiotic, where combinations with gentamicin showed a better outcome compared to vancomycin. Regarding the resistant strain, daptomycin had a superior antibiofilm effect than fosfomycin.https://www.mdpi.com/1999-4923/14/9/1779<i>Enterococcus faecalis</i>biofilmpersistent root canal infectionbacteriophage isolationbacteriophage-antibiotic combinationisothermal microcalorimetry |
| spellingShingle | Marie Voit Andrej Trampuz Mercedes Gonzalez Moreno In Vitro Evaluation of Five Newly Isolated Bacteriophages against <i>E. faecalis</i> Biofilm for Their Potential Use against Post-Treatment Apical Periodontitis Pharmaceutics <i>Enterococcus faecalis</i> biofilm persistent root canal infection bacteriophage isolation bacteriophage-antibiotic combination isothermal microcalorimetry |
| title | In Vitro Evaluation of Five Newly Isolated Bacteriophages against <i>E. faecalis</i> Biofilm for Their Potential Use against Post-Treatment Apical Periodontitis |
| title_full | In Vitro Evaluation of Five Newly Isolated Bacteriophages against <i>E. faecalis</i> Biofilm for Their Potential Use against Post-Treatment Apical Periodontitis |
| title_fullStr | In Vitro Evaluation of Five Newly Isolated Bacteriophages against <i>E. faecalis</i> Biofilm for Their Potential Use against Post-Treatment Apical Periodontitis |
| title_full_unstemmed | In Vitro Evaluation of Five Newly Isolated Bacteriophages against <i>E. faecalis</i> Biofilm for Their Potential Use against Post-Treatment Apical Periodontitis |
| title_short | In Vitro Evaluation of Five Newly Isolated Bacteriophages against <i>E. faecalis</i> Biofilm for Their Potential Use against Post-Treatment Apical Periodontitis |
| title_sort | in vitro evaluation of five newly isolated bacteriophages against i e faecalis i biofilm for their potential use against post treatment apical periodontitis |
| topic | <i>Enterococcus faecalis</i> biofilm persistent root canal infection bacteriophage isolation bacteriophage-antibiotic combination isothermal microcalorimetry |
| url | https://www.mdpi.com/1999-4923/14/9/1779 |
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