Cannabidiol attenuates inflammatory impairment of intestinal cells expanding biomaterial-based therapeutic approaches
Cannabis-based biomaterials have the potential to deliver anti-inflammatory therapeutics specifically to desired cells, tissues, and organs, enhancing drug delivery and the effectiveness of anti-inflammatory treatment while minimizing toxicity. As a major component of Cannabis, Cannabidiol (CBD) has...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-12-01
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| Series: | Materials Today Bio |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006423002685 |
| _version_ | 1797674143701794816 |
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| author | Elisa Boehm Linda Droessler Salah Amasheh |
| author_facet | Elisa Boehm Linda Droessler Salah Amasheh |
| author_sort | Elisa Boehm |
| collection | DOAJ |
| description | Cannabis-based biomaterials have the potential to deliver anti-inflammatory therapeutics specifically to desired cells, tissues, and organs, enhancing drug delivery and the effectiveness of anti-inflammatory treatment while minimizing toxicity. As a major component of Cannabis, Cannabidiol (CBD) has gained major attention in recent years because of its potential therapeutic properties, e.g., for restoring a disturbed barrier resulting from inflammatory conditions. The aim of this study was to test the hypothesis that CBD has beneficial effects under normal and inflammatory conditions in the established non-transformed intestinal epithelial cell model IPEC-J2. CBD induced a significant increase in transepithelial electrical resistance (TER) values and a decrease in the paracellular permeability of [³H]-D-Mannitol, indicating a strengthening effect on the barrier. Under inflammatory conditions induced by tumor necrosis factor alpha (TNFα), CBD stabilized the TER and mitigated the increase in paracellular permeability. Additionally, CBD prevented the barrier-disrupting effects of TNFα on the distribution and localization of sealing TJ proteins. CBD also affected the expression of TNF receptors. These findings demonstrate the potential of CBD as a component of Cannabis-based biomaterials used in the development of novel therapeutic approaches against inflammatory pathogenesis. |
| first_indexed | 2024-03-11T21:54:50Z |
| format | Article |
| id | doaj.art-87da1bd29932411aacd4fc4a6b023a85 |
| institution | Directory Open Access Journal |
| issn | 2590-0064 |
| language | English |
| last_indexed | 2024-03-11T21:54:50Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj.art-87da1bd29932411aacd4fc4a6b023a852023-09-26T04:12:19ZengElsevierMaterials Today Bio2590-00642023-12-0123100808Cannabidiol attenuates inflammatory impairment of intestinal cells expanding biomaterial-based therapeutic approachesElisa Boehm0Linda Droessler1Salah Amasheh2Institute of Veterinary Physiology, School of Veterinary Medicine, Freie Universität Berlin, Oertzenweg 19b, 14163, Berlin, GermanyInstitute of Veterinary Physiology, School of Veterinary Medicine, Freie Universität Berlin, Oertzenweg 19b, 14163, Berlin, GermanyCorresponding author.; Institute of Veterinary Physiology, School of Veterinary Medicine, Freie Universität Berlin, Oertzenweg 19b, 14163, Berlin, GermanyCannabis-based biomaterials have the potential to deliver anti-inflammatory therapeutics specifically to desired cells, tissues, and organs, enhancing drug delivery and the effectiveness of anti-inflammatory treatment while minimizing toxicity. As a major component of Cannabis, Cannabidiol (CBD) has gained major attention in recent years because of its potential therapeutic properties, e.g., for restoring a disturbed barrier resulting from inflammatory conditions. The aim of this study was to test the hypothesis that CBD has beneficial effects under normal and inflammatory conditions in the established non-transformed intestinal epithelial cell model IPEC-J2. CBD induced a significant increase in transepithelial electrical resistance (TER) values and a decrease in the paracellular permeability of [³H]-D-Mannitol, indicating a strengthening effect on the barrier. Under inflammatory conditions induced by tumor necrosis factor alpha (TNFα), CBD stabilized the TER and mitigated the increase in paracellular permeability. Additionally, CBD prevented the barrier-disrupting effects of TNFα on the distribution and localization of sealing TJ proteins. CBD also affected the expression of TNF receptors. These findings demonstrate the potential of CBD as a component of Cannabis-based biomaterials used in the development of novel therapeutic approaches against inflammatory pathogenesis.http://www.sciencedirect.com/science/article/pii/S2590006423002685CannabisEpithelial barrierCannabidiolTumor necrosis factorTight junctionsClaudins |
| spellingShingle | Elisa Boehm Linda Droessler Salah Amasheh Cannabidiol attenuates inflammatory impairment of intestinal cells expanding biomaterial-based therapeutic approaches Materials Today Bio Cannabis Epithelial barrier Cannabidiol Tumor necrosis factor Tight junctions Claudins |
| title | Cannabidiol attenuates inflammatory impairment of intestinal cells expanding biomaterial-based therapeutic approaches |
| title_full | Cannabidiol attenuates inflammatory impairment of intestinal cells expanding biomaterial-based therapeutic approaches |
| title_fullStr | Cannabidiol attenuates inflammatory impairment of intestinal cells expanding biomaterial-based therapeutic approaches |
| title_full_unstemmed | Cannabidiol attenuates inflammatory impairment of intestinal cells expanding biomaterial-based therapeutic approaches |
| title_short | Cannabidiol attenuates inflammatory impairment of intestinal cells expanding biomaterial-based therapeutic approaches |
| title_sort | cannabidiol attenuates inflammatory impairment of intestinal cells expanding biomaterial based therapeutic approaches |
| topic | Cannabis Epithelial barrier Cannabidiol Tumor necrosis factor Tight junctions Claudins |
| url | http://www.sciencedirect.com/science/article/pii/S2590006423002685 |
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