Hsa_circ_0051079 functions as an oncogene by regulating miR-26a-5p/TGF-β1 in osteosarcoma
Abstract Background Osteosarcoma is a most common bone malignant tumor which threatens children and adolescents. Circular RNAs (circRNAs) fundamentally play essential roles in the progress and development of human cancers by sponging with microRNAs (miRNAs). However, the role of circRNAs in osteosar...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-11-01
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| Series: | Cell & Bioscience |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13578-019-0355-2 |
| _version_ | 1818826531718299648 |
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| author | Zuojun Zhang Ming Zhao Guojie Wang |
| author_facet | Zuojun Zhang Ming Zhao Guojie Wang |
| author_sort | Zuojun Zhang |
| collection | DOAJ |
| description | Abstract Background Osteosarcoma is a most common bone malignant tumor which threatens children and adolescents. Circular RNAs (circRNAs) fundamentally play essential roles in the progress and development of human cancers by sponging with microRNAs (miRNAs). However, the role of circRNAs in osteosarcoma is not clear. The aim of the study was to investigate the roles and molecular mechanism of circRNAs in osteosarcoma. Results The data from qRT-PCR showed that circ_0051079 expression was higher in osteosarcoma cells and tissues compared to their normal controls. Meanwhile, bioinformatic analysis indicated that circ_0051079 was a sponge of miR-26a-5p, which was verified by luciferase activity assay. Subsequently, TGF-β1 was verified as a putative target mRNA of miR-26a-5p by luciferase assay. Cellular function assays were conducted and the findings revealed that circ_0051079/miR-26a-5p/TGF-β1 regulated osteosarcoma proliferation and metastasis. Conclusion The study demonstrated that circ_0051079 could act as an oncogene via regulating miR-26a-5p/TGF-β1 and a potential biomarker for osteosarcoma diagnose. |
| first_indexed | 2024-12-19T00:29:09Z |
| format | Article |
| id | doaj.art-87dd394b06a14459bc7e4db2b9b9023d |
| institution | Directory Open Access Journal |
| issn | 2045-3701 |
| language | English |
| last_indexed | 2024-12-19T00:29:09Z |
| publishDate | 2019-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Cell & Bioscience |
| spelling | doaj.art-87dd394b06a14459bc7e4db2b9b9023d2022-12-21T20:45:10ZengBMCCell & Bioscience2045-37012019-11-019111110.1186/s13578-019-0355-2Hsa_circ_0051079 functions as an oncogene by regulating miR-26a-5p/TGF-β1 in osteosarcomaZuojun Zhang0Ming Zhao1Guojie Wang2Upper Limb Injury Treatment Center, Luoyang Orthopedic Hospital of Henan ProvinceUpper Limb Injury Treatment Center, Luoyang Orthopedic Hospital of Henan ProvinceUpper Limb Injury Treatment Center, Luoyang Orthopedic Hospital of Henan ProvinceAbstract Background Osteosarcoma is a most common bone malignant tumor which threatens children and adolescents. Circular RNAs (circRNAs) fundamentally play essential roles in the progress and development of human cancers by sponging with microRNAs (miRNAs). However, the role of circRNAs in osteosarcoma is not clear. The aim of the study was to investigate the roles and molecular mechanism of circRNAs in osteosarcoma. Results The data from qRT-PCR showed that circ_0051079 expression was higher in osteosarcoma cells and tissues compared to their normal controls. Meanwhile, bioinformatic analysis indicated that circ_0051079 was a sponge of miR-26a-5p, which was verified by luciferase activity assay. Subsequently, TGF-β1 was verified as a putative target mRNA of miR-26a-5p by luciferase assay. Cellular function assays were conducted and the findings revealed that circ_0051079/miR-26a-5p/TGF-β1 regulated osteosarcoma proliferation and metastasis. Conclusion The study demonstrated that circ_0051079 could act as an oncogene via regulating miR-26a-5p/TGF-β1 and a potential biomarker for osteosarcoma diagnose.https://doi.org/10.1186/s13578-019-0355-2Hsa_circ_0051079miR-26a-5pTGF-β1Osteosarcoma |
| spellingShingle | Zuojun Zhang Ming Zhao Guojie Wang Hsa_circ_0051079 functions as an oncogene by regulating miR-26a-5p/TGF-β1 in osteosarcoma Cell & Bioscience Hsa_circ_0051079 miR-26a-5p TGF-β1 Osteosarcoma |
| title | Hsa_circ_0051079 functions as an oncogene by regulating miR-26a-5p/TGF-β1 in osteosarcoma |
| title_full | Hsa_circ_0051079 functions as an oncogene by regulating miR-26a-5p/TGF-β1 in osteosarcoma |
| title_fullStr | Hsa_circ_0051079 functions as an oncogene by regulating miR-26a-5p/TGF-β1 in osteosarcoma |
| title_full_unstemmed | Hsa_circ_0051079 functions as an oncogene by regulating miR-26a-5p/TGF-β1 in osteosarcoma |
| title_short | Hsa_circ_0051079 functions as an oncogene by regulating miR-26a-5p/TGF-β1 in osteosarcoma |
| title_sort | hsa circ 0051079 functions as an oncogene by regulating mir 26a 5p tgf β1 in osteosarcoma |
| topic | Hsa_circ_0051079 miR-26a-5p TGF-β1 Osteosarcoma |
| url | https://doi.org/10.1186/s13578-019-0355-2 |
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