Isolation and Quantification of Plant Extracellular Vesicles

Extracellular vesicles (EVs) play an important role in intercellular communication by transporting proteins and RNA. While plant cells secrete EVs, they have only recently been isolated and questions regarding their biogenesis, release, uptake and function remain unanswered. Here, we present a detai...

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Bibliografische gegevens
Hoofdauteurs: Brian Rutter, Katie Rutter, Roger Innes
Formaat: Artikel
Taal:English
Gepubliceerd in: Bio-protocol LLC 2017-09-01
Reeks:Bio-Protocol
Online toegang:https://bio-protocol.org/en/bpdetail?id=2533&type=0
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author Brian Rutter
Katie Rutter
Roger Innes
author_facet Brian Rutter
Katie Rutter
Roger Innes
author_sort Brian Rutter
collection DOAJ
description Extracellular vesicles (EVs) play an important role in intercellular communication by transporting proteins and RNA. While plant cells secrete EVs, they have only recently been isolated and questions regarding their biogenesis, release, uptake and function remain unanswered. Here, we present a detailed protocol for isolating EVs from the apoplastic wash of Arabidopsis thaliana leaves. The isolated EVs can be quantified using a fluorometric dye to assess total membrane content.
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spelling doaj.art-87de37f1ab8743f88f78cdcde6cb15602024-06-05T01:44:20ZengBio-protocol LLCBio-Protocol2331-83252017-09-0171710.21769/BioProtoc.2533Isolation and Quantification of Plant Extracellular VesiclesBrian Rutter0Katie Rutter1Roger Innes2Department of Biology, Indiana University, Bloomington, IN, USADepartment of Biology, Indiana University, Bloomington, IN, USADepartment of Biology, Indiana University, Bloomington, IN, USAExtracellular vesicles (EVs) play an important role in intercellular communication by transporting proteins and RNA. While plant cells secrete EVs, they have only recently been isolated and questions regarding their biogenesis, release, uptake and function remain unanswered. Here, we present a detailed protocol for isolating EVs from the apoplastic wash of Arabidopsis thaliana leaves. The isolated EVs can be quantified using a fluorometric dye to assess total membrane content. https://bio-protocol.org/en/bpdetail?id=2533&type=0
spellingShingle Brian Rutter
Katie Rutter
Roger Innes
Isolation and Quantification of Plant Extracellular Vesicles
Bio-Protocol
title Isolation and Quantification of Plant Extracellular Vesicles
title_full Isolation and Quantification of Plant Extracellular Vesicles
title_fullStr Isolation and Quantification of Plant Extracellular Vesicles
title_full_unstemmed Isolation and Quantification of Plant Extracellular Vesicles
title_short Isolation and Quantification of Plant Extracellular Vesicles
title_sort isolation and quantification of plant extracellular vesicles
url https://bio-protocol.org/en/bpdetail?id=2533&type=0
work_keys_str_mv AT brianrutter isolationandquantificationofplantextracellularvesicles
AT katierutter isolationandquantificationofplantextracellularvesicles
AT rogerinnes isolationandquantificationofplantextracellularvesicles