Isolation and Quantification of Plant Extracellular Vesicles
Extracellular vesicles (EVs) play an important role in intercellular communication by transporting proteins and RNA. While plant cells secrete EVs, they have only recently been isolated and questions regarding their biogenesis, release, uptake and function remain unanswered. Here, we present a detai...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Bio-protocol LLC
2017-09-01
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| Series: | Bio-Protocol |
| Online Access: | https://bio-protocol.org/en/bpdetail?id=2533&type=0 |
| _version_ | 1831869035651792896 |
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| author | Brian Rutter Katie Rutter Roger Innes |
| author_facet | Brian Rutter Katie Rutter Roger Innes |
| author_sort | Brian Rutter |
| collection | DOAJ |
| description | Extracellular vesicles (EVs) play an important role in intercellular communication by transporting proteins and RNA. While plant cells secrete EVs, they have only recently been isolated and questions regarding their biogenesis, release, uptake and function remain unanswered. Here, we present a detailed protocol for isolating EVs from the apoplastic wash of Arabidopsis thaliana leaves. The isolated EVs can be quantified using a fluorometric dye to assess total membrane content. |
| first_indexed | 2025-03-21T19:03:58Z |
| format | Article |
| id | doaj.art-87de37f1ab8743f88f78cdcde6cb1560 |
| institution | Directory Open Access Journal |
| issn | 2331-8325 |
| language | English |
| last_indexed | 2025-03-21T19:03:58Z |
| publishDate | 2017-09-01 |
| publisher | Bio-protocol LLC |
| record_format | Article |
| series | Bio-Protocol |
| spelling | doaj.art-87de37f1ab8743f88f78cdcde6cb15602024-06-05T01:44:20ZengBio-protocol LLCBio-Protocol2331-83252017-09-0171710.21769/BioProtoc.2533Isolation and Quantification of Plant Extracellular VesiclesBrian Rutter0Katie Rutter1Roger Innes2Department of Biology, Indiana University, Bloomington, IN, USADepartment of Biology, Indiana University, Bloomington, IN, USADepartment of Biology, Indiana University, Bloomington, IN, USAExtracellular vesicles (EVs) play an important role in intercellular communication by transporting proteins and RNA. While plant cells secrete EVs, they have only recently been isolated and questions regarding their biogenesis, release, uptake and function remain unanswered. Here, we present a detailed protocol for isolating EVs from the apoplastic wash of Arabidopsis thaliana leaves. The isolated EVs can be quantified using a fluorometric dye to assess total membrane content. https://bio-protocol.org/en/bpdetail?id=2533&type=0 |
| spellingShingle | Brian Rutter Katie Rutter Roger Innes Isolation and Quantification of Plant Extracellular Vesicles Bio-Protocol |
| title | Isolation and Quantification of Plant Extracellular Vesicles |
| title_full | Isolation and Quantification of Plant Extracellular Vesicles |
| title_fullStr | Isolation and Quantification of Plant Extracellular Vesicles |
| title_full_unstemmed | Isolation and Quantification of Plant Extracellular Vesicles |
| title_short | Isolation and Quantification of Plant Extracellular Vesicles |
| title_sort | isolation and quantification of plant extracellular vesicles |
| url | https://bio-protocol.org/en/bpdetail?id=2533&type=0 |
| work_keys_str_mv | AT brianrutter isolationandquantificationofplantextracellularvesicles AT katierutter isolationandquantificationofplantextracellularvesicles AT rogerinnes isolationandquantificationofplantextracellularvesicles |