<i>Haberlea rhodopensis</i> Extract Tunes the Cellular Response to Stress by Modulating DNA Damage, Redox Components, and Gene Expression
Ionizing radiation (IR) and reactive oxygen species (ROS)-induced oxidative stress can cause damage to cellular biomolecules, including DNA, proteins, and lipids. These harmful effects can compromise essential cellular functions and significantly raise the risk of metabolic dysfunction, accumulation...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/21/15964 |
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| author | Dessislava Staneva Neli Dimitrova Borislav Popov Albena Alexandrova Milena Georgieva George Miloshev |
| author_facet | Dessislava Staneva Neli Dimitrova Borislav Popov Albena Alexandrova Milena Georgieva George Miloshev |
| author_sort | Dessislava Staneva |
| collection | DOAJ |
| description | Ionizing radiation (IR) and reactive oxygen species (ROS)-induced oxidative stress can cause damage to cellular biomolecules, including DNA, proteins, and lipids. These harmful effects can compromise essential cellular functions and significantly raise the risk of metabolic dysfunction, accumulation of harmful mutations, genome instability, cancer, accelerated cellular senescence, and even death. Here, we present an investigation of HeLa cancer cells’ early response to gamma IR (γ-IR) and oxidative stress after preincubation of the cells with natural extracts of the resurrection plant <i>Haberlea rhodopensis</i>. In light of the superior protection offered by plant extracts against radiation and oxidative stress, we investigated the cellular defence mechanisms involved in such protection. Specifically, we sought to evaluate the molecular effects of <i>H. rhodopensis</i> extract (HRE) on cells subjected to genotoxic stress by examining the components of the redox pathway and quantifying the transcription levels of several critical genes associated with DNA repair, cell cycle regulation, and apoptosis. The influence of HRE on genome integrity and the cell cycle was also studied via comet assay and flow cytometry. Our findings demonstrate that HREs can effectively modulate the cellular response to genotoxic and oxidative stress within the first two hours following exposure, thereby reducing the severity of such stress. Furthermore, we observed the specificity of genoprotective HRE doses depending on the source of the applied genotoxic stress. |
| first_indexed | 2024-03-11T11:27:30Z |
| format | Article |
| id | doaj.art-87de7f8f849a42a6aa2b579cceece1e5 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T11:27:30Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-87de7f8f849a42a6aa2b579cceece1e52023-11-10T15:05:57ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-11-0124211596410.3390/ijms242115964<i>Haberlea rhodopensis</i> Extract Tunes the Cellular Response to Stress by Modulating DNA Damage, Redox Components, and Gene ExpressionDessislava Staneva0Neli Dimitrova1Borislav Popov2Albena Alexandrova3Milena Georgieva4George Miloshev5Laboratory of Molecular Genetics, Epigenetics and Longevity, Institute of Molecular Biology “Roumen Tsanev”, Bulgarian Academy of Sciences, 1113 Sofia, BulgariaDepartment of Molecular Biology, Immunology and Medical Genetics, Faculty of Medicine, Trakia University, 6000 Stara Zagora, BulgariaDepartment of Molecular Biology, Immunology and Medical Genetics, Faculty of Medicine, Trakia University, 6000 Stara Zagora, BulgariaLaboratory of Free Radical Processes, Institute of Neurobiology, Bulgarian Academy of Sciences, 1113 Sofia, BulgariaLaboratory of Molecular Genetics, Epigenetics and Longevity, Institute of Molecular Biology “Roumen Tsanev”, Bulgarian Academy of Sciences, 1113 Sofia, BulgariaLaboratory of Molecular Genetics, Epigenetics and Longevity, Institute of Molecular Biology “Roumen Tsanev”, Bulgarian Academy of Sciences, 1113 Sofia, BulgariaIonizing radiation (IR) and reactive oxygen species (ROS)-induced oxidative stress can cause damage to cellular biomolecules, including DNA, proteins, and lipids. These harmful effects can compromise essential cellular functions and significantly raise the risk of metabolic dysfunction, accumulation of harmful mutations, genome instability, cancer, accelerated cellular senescence, and even death. Here, we present an investigation of HeLa cancer cells’ early response to gamma IR (γ-IR) and oxidative stress after preincubation of the cells with natural extracts of the resurrection plant <i>Haberlea rhodopensis</i>. In light of the superior protection offered by plant extracts against radiation and oxidative stress, we investigated the cellular defence mechanisms involved in such protection. Specifically, we sought to evaluate the molecular effects of <i>H. rhodopensis</i> extract (HRE) on cells subjected to genotoxic stress by examining the components of the redox pathway and quantifying the transcription levels of several critical genes associated with DNA repair, cell cycle regulation, and apoptosis. The influence of HRE on genome integrity and the cell cycle was also studied via comet assay and flow cytometry. Our findings demonstrate that HREs can effectively modulate the cellular response to genotoxic and oxidative stress within the first two hours following exposure, thereby reducing the severity of such stress. Furthermore, we observed the specificity of genoprotective HRE doses depending on the source of the applied genotoxic stress.https://www.mdpi.com/1422-0067/24/21/15964<i>Haberlea rhodopensis</i>gamma irradiationoxidative stressgenotoxicityantioxidantgene transcription |
| spellingShingle | Dessislava Staneva Neli Dimitrova Borislav Popov Albena Alexandrova Milena Georgieva George Miloshev <i>Haberlea rhodopensis</i> Extract Tunes the Cellular Response to Stress by Modulating DNA Damage, Redox Components, and Gene Expression International Journal of Molecular Sciences <i>Haberlea rhodopensis</i> gamma irradiation oxidative stress genotoxicity antioxidant gene transcription |
| title | <i>Haberlea rhodopensis</i> Extract Tunes the Cellular Response to Stress by Modulating DNA Damage, Redox Components, and Gene Expression |
| title_full | <i>Haberlea rhodopensis</i> Extract Tunes the Cellular Response to Stress by Modulating DNA Damage, Redox Components, and Gene Expression |
| title_fullStr | <i>Haberlea rhodopensis</i> Extract Tunes the Cellular Response to Stress by Modulating DNA Damage, Redox Components, and Gene Expression |
| title_full_unstemmed | <i>Haberlea rhodopensis</i> Extract Tunes the Cellular Response to Stress by Modulating DNA Damage, Redox Components, and Gene Expression |
| title_short | <i>Haberlea rhodopensis</i> Extract Tunes the Cellular Response to Stress by Modulating DNA Damage, Redox Components, and Gene Expression |
| title_sort | i haberlea rhodopensis i extract tunes the cellular response to stress by modulating dna damage redox components and gene expression |
| topic | <i>Haberlea rhodopensis</i> gamma irradiation oxidative stress genotoxicity antioxidant gene transcription |
| url | https://www.mdpi.com/1422-0067/24/21/15964 |
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