Cytotoxicity study of Houttuynia cordata Thunb crude extracts on HL-60 and Molt4 leukemic cell line
Objective: To investigate the effect of crude extract obtained from stem and leaf of Houttuynia cordata Thunb (Plu-Kao) on HL-60 and Molt4 leukemic cell line compared to normal peripheral blood mononuclear cells (PBMCs). Method: The stem and leaf of Plu-Kao were extracted with 80% eth...
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| Format: | Article |
| Language: | English |
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Chaing Mai University
2010-01-01
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| Series: | Journal of Associated Medical Sciences |
| Subjects: | |
| Online Access: | https://www.tci-thaijo.org/index.php/bulletinAMS/article/view/60066 |
| _version_ | 1818416347513618432 |
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| author | Singkome Tima Bhakdee Phanphuech Songyot Anuchapreeda |
| author_facet | Singkome Tima Bhakdee Phanphuech Songyot Anuchapreeda |
| author_sort | Singkome Tima |
| collection | DOAJ |
| description | Objective: To investigate the effect of crude extract obtained from stem and leaf of Houttuynia cordata Thunb (Plu-Kao) on HL-60 and Molt4 leukemic cell line compared to normal peripheral blood mononuclear cells (PBMCs).
Method: The stem and leaf of Plu-Kao were extracted with 80% ethanol and various concentrations of the crude extracts were used to treat the HL-60, Molt4, and PBMCs. The cytotoxic effect of crude extracts was investigated by MTT assay.
Results: It was found that crude Plu-Kao extract form stem and leaf showed the cytotoxic effect on HL-60 and Molt4 with inhibitory concentration at 50% (IC50) between 500-800 μg/mL. Interestingly, the crude extracts did not show the cytotoxic affect on PBMCs with the IC50 > 800 μg/mL.
Conclusion: The crude Plu-Kao extracts showed the cytotoxic effect on both leukemic cells (HL-60 and Molt4) but not on PBMCs. Thus, the further investigation of the extracts should be performed to identify an active ingredient and develop chemotherapeutic agent for leukemia treatment. Bull Chiang Mai Assoc Med Sci 2010; 43: 30-38. |
| first_indexed | 2024-12-14T11:49:27Z |
| format | Article |
| id | doaj.art-87e3a2cd1f4744a28395c4aadd80821a |
| institution | Directory Open Access Journal |
| issn | 2539-6056 2539-6056 |
| language | English |
| last_indexed | 2024-12-14T11:49:27Z |
| publishDate | 2010-01-01 |
| publisher | Chaing Mai University |
| record_format | Article |
| series | Journal of Associated Medical Sciences |
| spelling | doaj.art-87e3a2cd1f4744a28395c4aadd80821a2022-12-21T23:02:26ZengChaing Mai UniversityJournal of Associated Medical Sciences2539-60562539-60562010-01-01431303060066Cytotoxicity study of Houttuynia cordata Thunb crude extracts on HL-60 and Molt4 leukemic cell lineSingkome Tima0Bhakdee Phanphuech1Songyot Anuchapreeda21 Division of Clinical Microscopy, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University. 2 Umphang hospital, Umphang, Tak, Thailand.1 Division of Clinical Microscopy, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University. 2 Umphang hospital, Umphang, Tak, Thailand.1 Division of Clinical Microscopy, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University. 2 Umphang hospital, Umphang, Tak, Thailand.Objective: To investigate the effect of crude extract obtained from stem and leaf of Houttuynia cordata Thunb (Plu-Kao) on HL-60 and Molt4 leukemic cell line compared to normal peripheral blood mononuclear cells (PBMCs). Method: The stem and leaf of Plu-Kao were extracted with 80% ethanol and various concentrations of the crude extracts were used to treat the HL-60, Molt4, and PBMCs. The cytotoxic effect of crude extracts was investigated by MTT assay. Results: It was found that crude Plu-Kao extract form stem and leaf showed the cytotoxic effect on HL-60 and Molt4 with inhibitory concentration at 50% (IC50) between 500-800 μg/mL. Interestingly, the crude extracts did not show the cytotoxic affect on PBMCs with the IC50 > 800 μg/mL. Conclusion: The crude Plu-Kao extracts showed the cytotoxic effect on both leukemic cells (HL-60 and Molt4) but not on PBMCs. Thus, the further investigation of the extracts should be performed to identify an active ingredient and develop chemotherapeutic agent for leukemia treatment. Bull Chiang Mai Assoc Med Sci 2010; 43: 30-38.https://www.tci-thaijo.org/index.php/bulletinAMS/article/view/60066Houttuynia cordata Thunb (Plu-Kao)LeukemiaHL-60Molt4MTT assay |
| spellingShingle | Singkome Tima Bhakdee Phanphuech Songyot Anuchapreeda Cytotoxicity study of Houttuynia cordata Thunb crude extracts on HL-60 and Molt4 leukemic cell line Journal of Associated Medical Sciences Houttuynia cordata Thunb (Plu-Kao) Leukemia HL-60 Molt4 MTT assay |
| title | Cytotoxicity study of Houttuynia cordata Thunb crude extracts on HL-60 and Molt4 leukemic cell line |
| title_full | Cytotoxicity study of Houttuynia cordata Thunb crude extracts on HL-60 and Molt4 leukemic cell line |
| title_fullStr | Cytotoxicity study of Houttuynia cordata Thunb crude extracts on HL-60 and Molt4 leukemic cell line |
| title_full_unstemmed | Cytotoxicity study of Houttuynia cordata Thunb crude extracts on HL-60 and Molt4 leukemic cell line |
| title_short | Cytotoxicity study of Houttuynia cordata Thunb crude extracts on HL-60 and Molt4 leukemic cell line |
| title_sort | cytotoxicity study of houttuynia cordata thunb crude extracts on hl 60 and molt4 leukemic cell line |
| topic | Houttuynia cordata Thunb (Plu-Kao) Leukemia HL-60 Molt4 MTT assay |
| url | https://www.tci-thaijo.org/index.php/bulletinAMS/article/view/60066 |
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