Gene knockdown by RNAi in the pea aphid <it>Acyrthosiphon pisum</it>
<p>Abstract</p> <p>Background</p> <p>RNA interference (RNAi) is a powerful method to inhibit gene expression in a sequence specific manner.</p> <p>Results</p> <p>Here, we described the development of RNAi by micro-injection of double-stranded RNA...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2007-09-01
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| Series: | BMC Biotechnology |
| Online Access: | http://www.biomedcentral.com/1472-6750/7/63 |
| _version_ | 1818826873380012032 |
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| author | Rispe Claude Christophides Georges K Bonhomme Joël Le Trionnaire Gaël Jaubert-Possamai Stéphanie Tagu Denis |
| author_facet | Rispe Claude Christophides Georges K Bonhomme Joël Le Trionnaire Gaël Jaubert-Possamai Stéphanie Tagu Denis |
| author_sort | Rispe Claude |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>RNA interference (RNAi) is a powerful method to inhibit gene expression in a sequence specific manner.</p> <p>Results</p> <p>Here, we described the development of RNAi by micro-injection of double-stranded RNA (dsRNA) in the pea aphid <it>Acyrthosiphon pisum</it>. Injection of dsRNA into whole aphid body induced the silencing of two marker genes with different expression patterns: the ubiquitously expressed <it>Ap-crt </it>genes encoding a calreticulin and the gut specific <it>Ap-cath-L </it>gene encoding a cathepsin-L. Time-course analysis of the silencing showed similar temporal patterns for both genes: inhibition started at 1 day after injection, reached its maximum at 5 days and stopped at 7 days. A comparable 40% decrease of gene expression was observed for <it>Ap-crt </it>and <it>Ap-cath-L</it>.</p> <p>Conclusion</p> <p>The pea aphid is the first Hemipteran insect for which genome sequence will be available soon. The gene knockdown technique developed in this study will be an essential post-genomic tool for further investigations in aphidology.</p> |
| first_indexed | 2024-12-19T00:34:35Z |
| format | Article |
| id | doaj.art-87e3b3bbc43f485a98298d326b86691d |
| institution | Directory Open Access Journal |
| issn | 1472-6750 |
| language | English |
| last_indexed | 2024-12-19T00:34:35Z |
| publishDate | 2007-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Biotechnology |
| spelling | doaj.art-87e3b3bbc43f485a98298d326b86691d2022-12-21T20:44:53ZengBMCBMC Biotechnology1472-67502007-09-01716310.1186/1472-6750-7-63Gene knockdown by RNAi in the pea aphid <it>Acyrthosiphon pisum</it>Rispe ClaudeChristophides Georges KBonhomme JoëlLe Trionnaire GaëlJaubert-Possamai StéphanieTagu Denis<p>Abstract</p> <p>Background</p> <p>RNA interference (RNAi) is a powerful method to inhibit gene expression in a sequence specific manner.</p> <p>Results</p> <p>Here, we described the development of RNAi by micro-injection of double-stranded RNA (dsRNA) in the pea aphid <it>Acyrthosiphon pisum</it>. Injection of dsRNA into whole aphid body induced the silencing of two marker genes with different expression patterns: the ubiquitously expressed <it>Ap-crt </it>genes encoding a calreticulin and the gut specific <it>Ap-cath-L </it>gene encoding a cathepsin-L. Time-course analysis of the silencing showed similar temporal patterns for both genes: inhibition started at 1 day after injection, reached its maximum at 5 days and stopped at 7 days. A comparable 40% decrease of gene expression was observed for <it>Ap-crt </it>and <it>Ap-cath-L</it>.</p> <p>Conclusion</p> <p>The pea aphid is the first Hemipteran insect for which genome sequence will be available soon. The gene knockdown technique developed in this study will be an essential post-genomic tool for further investigations in aphidology.</p>http://www.biomedcentral.com/1472-6750/7/63 |
| spellingShingle | Rispe Claude Christophides Georges K Bonhomme Joël Le Trionnaire Gaël Jaubert-Possamai Stéphanie Tagu Denis Gene knockdown by RNAi in the pea aphid <it>Acyrthosiphon pisum</it> BMC Biotechnology |
| title | Gene knockdown by RNAi in the pea aphid <it>Acyrthosiphon pisum</it> |
| title_full | Gene knockdown by RNAi in the pea aphid <it>Acyrthosiphon pisum</it> |
| title_fullStr | Gene knockdown by RNAi in the pea aphid <it>Acyrthosiphon pisum</it> |
| title_full_unstemmed | Gene knockdown by RNAi in the pea aphid <it>Acyrthosiphon pisum</it> |
| title_short | Gene knockdown by RNAi in the pea aphid <it>Acyrthosiphon pisum</it> |
| title_sort | gene knockdown by rnai in the pea aphid it acyrthosiphon pisum it |
| url | http://www.biomedcentral.com/1472-6750/7/63 |
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