Synthesis and characterization of a novel chemically designed (Globo)3–DTPA–KLH antigen
Mehdi Hajmohammadi,1 Seyed Davar Siadat,2 Masoud Ghorbani,3,4,* Mehdi Shafiee Ardestani,5,* Shahram Teimourian,6 Vahid Asgari,3 Reza Ahangari Cohan,3 Mostafa Hajmohammadi,5 Akram Hajmohammadi,7 Ramezan Behzadi,8 Saied Rajab Nezhad,9 Nabiollah Namvar Asl10 1Department of Research and Biotechnology,...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2014-12-01
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| Series: | Drug Design, Development and Therapy |
| Online Access: | http://www.dovepress.com/synthesis-and-characterization-of-a-novel-chemically-designed-globo3nd-peer-reviewed-article-DDDT |
| _version_ | 1811248264481079296 |
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| author | Hajmohammadi M Siadat SD Ghorbani M Shafiee Ardestani M Teimourian S Asgari V Ahangari Cohan R Hajmohammadi M Hajmohammadi A Behzadi R Rajab Nezhad S Namvar Asl N |
| author_facet | Hajmohammadi M Siadat SD Ghorbani M Shafiee Ardestani M Teimourian S Asgari V Ahangari Cohan R Hajmohammadi M Hajmohammadi A Behzadi R Rajab Nezhad S Namvar Asl N |
| author_sort | Hajmohammadi M |
| collection | DOAJ |
| description | Mehdi Hajmohammadi,1 Seyed Davar Siadat,2 Masoud Ghorbani,3,4,* Mehdi Shafiee Ardestani,5,* Shahram Teimourian,6 Vahid Asgari,3 Reza Ahangari Cohan,3 Mostafa Hajmohammadi,5 Akram Hajmohammadi,7 Ramezan Behzadi,8 Saied Rajab Nezhad,9 Nabiollah Namvar Asl10 1Department of Research and Biotechnology, 2Department of Microbiology, 3Department of Virology, Pasteur Institute of Iran, Tehran Iran; 4Department of Virology, University of Ottawa, Ottawa, ON, Canada; 5Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran; 6Department of Medical Genetics, Iran University of Medical Sciences, Tehran, 7Faculty of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, 8Laboratory Animal Management of North Research Center, Pasteur Institute of Iran, 9Department of Research and Development, Alhavi Pharmaceutical, Tehran, 10Pasteur Institute of Iran, Department of Animal Sciences, Karaj, Iran *These authors contributed equally to this work Abstract: In recent years, many experiments have been conducted for the production and evaluation of anticancer glycoconjugated vaccines in developed countries and many achievements have been accomplished with Globo H derivatives. In the current experiment, a new chemically designed triplicate version of (Globo H)3–diethylenetriamine pentaacetic acid (DTPA)–KLH antigen was synthesized and characterized. Immunization with (Globo H)3-DTPA-KLH, a hexasaccharide that is a member of a family of antigenic carbohydrates that are highly expressed in various types of cancers conjugated with DTPA and KLH protein, induced a high level of antibody titer along with an elevated level of IL-4 in mice. Treatment of tumors with the collected sera from immunized mice decreased the tumor size in nude mice as well. None of the immunized mice illustrated any sign of tumor growth after injection of MCF-7 cells compared to the control animals. These findings, based on the newly presented structure of the Globo H antigen, lend exciting and promising evidence for clinical advancement in the development of a therapeutic vaccine in the future. Keywords: (Globo H)3-DTPA-KLH, glycoconjugate vaccines, breast cancer |
| first_indexed | 2024-04-12T15:23:52Z |
| format | Article |
| id | doaj.art-87e4c26e88e0432abadb9e4f60b53316 |
| institution | Directory Open Access Journal |
| issn | 1177-8881 |
| language | English |
| last_indexed | 2024-04-12T15:23:52Z |
| publishDate | 2014-12-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Drug Design, Development and Therapy |
| spelling | doaj.art-87e4c26e88e0432abadb9e4f60b533162022-12-22T03:27:20ZengDove Medical PressDrug Design, Development and Therapy1177-88812014-12-012015default21723919711Synthesis and characterization of a novel chemically designed (Globo)3–DTPA–KLH antigenHajmohammadi MSiadat SDGhorbani MShafiee Ardestani MTeimourian SAsgari VAhangari Cohan RHajmohammadi MHajmohammadi ABehzadi RRajab Nezhad SNamvar Asl NMehdi Hajmohammadi,1 Seyed Davar Siadat,2 Masoud Ghorbani,3,4,* Mehdi Shafiee Ardestani,5,* Shahram Teimourian,6 Vahid Asgari,3 Reza Ahangari Cohan,3 Mostafa Hajmohammadi,5 Akram Hajmohammadi,7 Ramezan Behzadi,8 Saied Rajab Nezhad,9 Nabiollah Namvar Asl10 1Department of Research and Biotechnology, 2Department of Microbiology, 3Department of Virology, Pasteur Institute of Iran, Tehran Iran; 4Department of Virology, University of Ottawa, Ottawa, ON, Canada; 5Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran; 6Department of Medical Genetics, Iran University of Medical Sciences, Tehran, 7Faculty of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, 8Laboratory Animal Management of North Research Center, Pasteur Institute of Iran, 9Department of Research and Development, Alhavi Pharmaceutical, Tehran, 10Pasteur Institute of Iran, Department of Animal Sciences, Karaj, Iran *These authors contributed equally to this work Abstract: In recent years, many experiments have been conducted for the production and evaluation of anticancer glycoconjugated vaccines in developed countries and many achievements have been accomplished with Globo H derivatives. In the current experiment, a new chemically designed triplicate version of (Globo H)3–diethylenetriamine pentaacetic acid (DTPA)–KLH antigen was synthesized and characterized. Immunization with (Globo H)3-DTPA-KLH, a hexasaccharide that is a member of a family of antigenic carbohydrates that are highly expressed in various types of cancers conjugated with DTPA and KLH protein, induced a high level of antibody titer along with an elevated level of IL-4 in mice. Treatment of tumors with the collected sera from immunized mice decreased the tumor size in nude mice as well. None of the immunized mice illustrated any sign of tumor growth after injection of MCF-7 cells compared to the control animals. These findings, based on the newly presented structure of the Globo H antigen, lend exciting and promising evidence for clinical advancement in the development of a therapeutic vaccine in the future. Keywords: (Globo H)3-DTPA-KLH, glycoconjugate vaccines, breast cancerhttp://www.dovepress.com/synthesis-and-characterization-of-a-novel-chemically-designed-globo3nd-peer-reviewed-article-DDDT |
| spellingShingle | Hajmohammadi M Siadat SD Ghorbani M Shafiee Ardestani M Teimourian S Asgari V Ahangari Cohan R Hajmohammadi M Hajmohammadi A Behzadi R Rajab Nezhad S Namvar Asl N Synthesis and characterization of a novel chemically designed (Globo)3–DTPA–KLH antigen Drug Design, Development and Therapy |
| title | Synthesis and characterization of a novel chemically designed (Globo)3–DTPA–KLH antigen |
| title_full | Synthesis and characterization of a novel chemically designed (Globo)3–DTPA–KLH antigen |
| title_fullStr | Synthesis and characterization of a novel chemically designed (Globo)3–DTPA–KLH antigen |
| title_full_unstemmed | Synthesis and characterization of a novel chemically designed (Globo)3–DTPA–KLH antigen |
| title_short | Synthesis and characterization of a novel chemically designed (Globo)3–DTPA–KLH antigen |
| title_sort | synthesis and characterization of a novel chemically designed globo 3 ndash dtpa ndash klh antigen |
| url | http://www.dovepress.com/synthesis-and-characterization-of-a-novel-chemically-designed-globo3nd-peer-reviewed-article-DDDT |
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