Sensitive fluorescence ELISA with streptavidin scaffolded DNA tetrads for the detection of Escherichia coli O157:H7
ABSTRACT: Escherichia coli O157:H7 poses a threat to humans. Traditional ELISA is not a sensitive method for the detection of E. coli O157:H7. Here, an efficient method was designed for improving the load capacity of alkaline phosphatase (ALP) with streptavidin scaffolded DNA tetrad (SS-DNAt). With...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | Journal of Dairy Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022030223003806 |
| _version_ | 1797741468680454144 |
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| author | Xudong Jing Shan Shan Keyu Xing Wenkai Cao Xiaoyue Xiao Daofeng Liu Weihua Lai |
| author_facet | Xudong Jing Shan Shan Keyu Xing Wenkai Cao Xiaoyue Xiao Daofeng Liu Weihua Lai |
| author_sort | Xudong Jing |
| collection | DOAJ |
| description | ABSTRACT: Escherichia coli O157:H7 poses a threat to humans. Traditional ELISA is not a sensitive method for the detection of E. coli O157:H7. Here, an efficient method was designed for improving the load capacity of alkaline phosphatase (ALP) with streptavidin scaffolded DNA tetrad (SS-DNAt). With more ALP, more ascorbic acid 2-phosphate was catalyzed to ascorbic acid that was used to synthesize fluorescence poly adenine-thymine–templated copper nanoclusters. Based on SS-DNAt, fluorescence ELISA was successfully proposed for improving the sensitivity for detection of E. coli O157:H7 in milk samples. The method showed a linear range of 104 to 106 cfu/mL. The limit of detection of fluorescence ELISA was 3.75 × 103 cfu/mL and 6.16-fold better than that of traditional ELISA. The recovery of the fluorescence ELISA was 86.7 to 93.6% with the coefficient of variation of 5.6 to 10.5% in milk. This method could be used to detect hazardous material in food. |
| first_indexed | 2024-03-12T14:27:27Z |
| format | Article |
| id | doaj.art-87e4d0cb66534a44aab31e8264f156c4 |
| institution | Directory Open Access Journal |
| issn | 0022-0302 |
| language | English |
| last_indexed | 2024-03-12T14:27:27Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Dairy Science |
| spelling | doaj.art-87e4d0cb66534a44aab31e8264f156c42023-08-18T04:30:22ZengElsevierJournal of Dairy Science0022-03022023-09-01106959305939Sensitive fluorescence ELISA with streptavidin scaffolded DNA tetrads for the detection of Escherichia coli O157:H7Xudong Jing0Shan Shan1Keyu Xing2Wenkai Cao3Xiaoyue Xiao4Daofeng Liu5Weihua Lai6State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaCollege of Life Science, National R&D Center for Freshwater Fish Processing, Jiangxi Normal University, Nanchang 330022, China; Jiangxi Province Key Laboratory of Diagnosing and Tracing of Foodborne Disease, Jiangxi Province Center for Disease Control and Prevention, Nanchang 330029, ChinaSchool of Food Science and Bioengineering, Changsha University of Science and Technology, Changsha 410114, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaJiangxi Province Key Laboratory of Diagnosing and Tracing of Foodborne Disease, Jiangxi Province Center for Disease Control and Prevention, Nanchang 330029, China; Corresponding authorsState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China; Corresponding authorsABSTRACT: Escherichia coli O157:H7 poses a threat to humans. Traditional ELISA is not a sensitive method for the detection of E. coli O157:H7. Here, an efficient method was designed for improving the load capacity of alkaline phosphatase (ALP) with streptavidin scaffolded DNA tetrad (SS-DNAt). With more ALP, more ascorbic acid 2-phosphate was catalyzed to ascorbic acid that was used to synthesize fluorescence poly adenine-thymine–templated copper nanoclusters. Based on SS-DNAt, fluorescence ELISA was successfully proposed for improving the sensitivity for detection of E. coli O157:H7 in milk samples. The method showed a linear range of 104 to 106 cfu/mL. The limit of detection of fluorescence ELISA was 3.75 × 103 cfu/mL and 6.16-fold better than that of traditional ELISA. The recovery of the fluorescence ELISA was 86.7 to 93.6% with the coefficient of variation of 5.6 to 10.5% in milk. This method could be used to detect hazardous material in food.http://www.sciencedirect.com/science/article/pii/S0022030223003806enzyme-linked immunosorbent assaystreptavidin scaffolded DNA tetradcopper nanoclustersEscherichia coli O157:H7 |
| spellingShingle | Xudong Jing Shan Shan Keyu Xing Wenkai Cao Xiaoyue Xiao Daofeng Liu Weihua Lai Sensitive fluorescence ELISA with streptavidin scaffolded DNA tetrads for the detection of Escherichia coli O157:H7 Journal of Dairy Science enzyme-linked immunosorbent assay streptavidin scaffolded DNA tetrad copper nanoclusters Escherichia coli O157:H7 |
| title | Sensitive fluorescence ELISA with streptavidin scaffolded DNA tetrads for the detection of Escherichia coli O157:H7 |
| title_full | Sensitive fluorescence ELISA with streptavidin scaffolded DNA tetrads for the detection of Escherichia coli O157:H7 |
| title_fullStr | Sensitive fluorescence ELISA with streptavidin scaffolded DNA tetrads for the detection of Escherichia coli O157:H7 |
| title_full_unstemmed | Sensitive fluorescence ELISA with streptavidin scaffolded DNA tetrads for the detection of Escherichia coli O157:H7 |
| title_short | Sensitive fluorescence ELISA with streptavidin scaffolded DNA tetrads for the detection of Escherichia coli O157:H7 |
| title_sort | sensitive fluorescence elisa with streptavidin scaffolded dna tetrads for the detection of escherichia coli o157 h7 |
| topic | enzyme-linked immunosorbent assay streptavidin scaffolded DNA tetrad copper nanoclusters Escherichia coli O157:H7 |
| url | http://www.sciencedirect.com/science/article/pii/S0022030223003806 |
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