Estimation of Phagocytic activity by normal human peripheral blood mononuclear cells on various oral isolates of Candida species – An in-vitro study
Polymorphonuclear neutrophils (PMN) and mononuclear phagocytes represent an important first line and effector function in control of Candida infections. The aim of the study is to determine the in-vitro phagocytic activity of human peripheral blood mononuclear cells against oral isolates of Candida...
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| Format: | Article |
| Language: | English |
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Poznan University of Medical Sciences
2024-02-01
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| Series: | Journal of Medical Science |
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| Online Access: | https://jms.ump.edu.pl/index.php/JMS/article/view/953 |
| _version_ | 1797335588264738816 |
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| author | Sreelakshmi P J Harish Kumar K S Jacob Kuruvilla Philip George Theckel |
| author_facet | Sreelakshmi P J Harish Kumar K S Jacob Kuruvilla Philip George Theckel |
| author_sort | Sreelakshmi P J |
| collection | DOAJ |
| description |
Polymorphonuclear neutrophils (PMN) and mononuclear phagocytes represent an important first line and effector function in control of Candida infections. The aim of the study is to determine the in-vitro phagocytic activity of human peripheral blood mononuclear cells against oral isolates of Candida species and its antifungal susceptibility. The study also evaluates the degree of respiratory burst activity of PBMCs. Phagocytic and lytic indices by PBMCs were determined for Candida spp. The respiratory burst activity was evaluated by nitroblue tetrazolium test. Antifungal disc diffusion susceptibility testing was performed.
A total of 100 Candida were isolated belonging to the species C.albicans, C. tropicalis, C.krusei and C.auris. Phagocytic and lytic indices of C.albicans was significant when compared to standard strain of C.albicans. For C.tropicalis and C.krusei phagocytic index was significant while lytic index was not significant when compared to standard strain. The inter species comparison of both the indices was not significant for the clinical isolates of Candida. A significant reduction in phagocytic activity was observed for clinical isolates of Candida spp. but lytic activity was variable when compared to the standard strain of C.albicans.
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| first_indexed | 2024-03-08T08:40:25Z |
| format | Article |
| id | doaj.art-87e5cf052f05464783a0562f1debc6c7 |
| institution | Directory Open Access Journal |
| issn | 2353-9798 2353-9801 |
| language | English |
| last_indexed | 2024-03-08T08:40:25Z |
| publishDate | 2024-02-01 |
| publisher | Poznan University of Medical Sciences |
| record_format | Article |
| series | Journal of Medical Science |
| spelling | doaj.art-87e5cf052f05464783a0562f1debc6c72024-02-01T19:06:53ZengPoznan University of Medical SciencesJournal of Medical Science2353-97982353-98012024-02-0110.20883/medical.e953Estimation of Phagocytic activity by normal human peripheral blood mononuclear cells on various oral isolates of Candida species – An in-vitro studySreelakshmi P J0Harish Kumar K S1https://orcid.org/0000-0002-0504-7794Jacob Kuruvilla2Philip George Theckel3Department of Medical Microbiology, School of Medical Education, Centre for Professional and Advanced Studies, Kottayam, Kerala, India1Department of Medical Microbiology, School of Medical Education, Centre for Professional and Advanced Studies, Kottayam, Kerala, IndiaDepartment of Public Health Dentistry, Mar Baselios Dental College, Ernakulam, Kerala, IndiaElectron Microscopy Unit, Faculty of Medicine, Kuwait University, Kuwait Polymorphonuclear neutrophils (PMN) and mononuclear phagocytes represent an important first line and effector function in control of Candida infections. The aim of the study is to determine the in-vitro phagocytic activity of human peripheral blood mononuclear cells against oral isolates of Candida species and its antifungal susceptibility. The study also evaluates the degree of respiratory burst activity of PBMCs. Phagocytic and lytic indices by PBMCs were determined for Candida spp. The respiratory burst activity was evaluated by nitroblue tetrazolium test. Antifungal disc diffusion susceptibility testing was performed. A total of 100 Candida were isolated belonging to the species C.albicans, C. tropicalis, C.krusei and C.auris. Phagocytic and lytic indices of C.albicans was significant when compared to standard strain of C.albicans. For C.tropicalis and C.krusei phagocytic index was significant while lytic index was not significant when compared to standard strain. The inter species comparison of both the indices was not significant for the clinical isolates of Candida. A significant reduction in phagocytic activity was observed for clinical isolates of Candida spp. but lytic activity was variable when compared to the standard strain of C.albicans. https://jms.ump.edu.pl/index.php/JMS/article/view/953Phagocytic activityCandida speciesRespiratory burstNitroblue tetrazolium testAntifungal susceptibility |
| spellingShingle | Sreelakshmi P J Harish Kumar K S Jacob Kuruvilla Philip George Theckel Estimation of Phagocytic activity by normal human peripheral blood mononuclear cells on various oral isolates of Candida species – An in-vitro study Journal of Medical Science Phagocytic activity Candida species Respiratory burst Nitroblue tetrazolium test Antifungal susceptibility |
| title | Estimation of Phagocytic activity by normal human peripheral blood mononuclear cells on various oral isolates of Candida species – An in-vitro study |
| title_full | Estimation of Phagocytic activity by normal human peripheral blood mononuclear cells on various oral isolates of Candida species – An in-vitro study |
| title_fullStr | Estimation of Phagocytic activity by normal human peripheral blood mononuclear cells on various oral isolates of Candida species – An in-vitro study |
| title_full_unstemmed | Estimation of Phagocytic activity by normal human peripheral blood mononuclear cells on various oral isolates of Candida species – An in-vitro study |
| title_short | Estimation of Phagocytic activity by normal human peripheral blood mononuclear cells on various oral isolates of Candida species – An in-vitro study |
| title_sort | estimation of phagocytic activity by normal human peripheral blood mononuclear cells on various oral isolates of candida species an in vitro study |
| topic | Phagocytic activity Candida species Respiratory burst Nitroblue tetrazolium test Antifungal susceptibility |
| url | https://jms.ump.edu.pl/index.php/JMS/article/view/953 |
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