Suppressive Effects of Bee Venom Acupuncture on Paclitaxel-Induced Neuropathic Pain in Rats: Mediation by Spinal α2-Adrenergic Receptor
Paclitaxel, a chemotherapy drug for solid tumors, induces peripheral painful neuropathy. Bee venom acupuncture (BVA) has been reported to have potent analgesic effects, which are known to be mediated by activation of spinal α-adrenergic receptor. Here, we investigated the effect of BVA on mechanical...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2017-10-01
|
| Series: | Toxins |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6651/9/11/351 |
| _version_ | 1817995133933584384 |
|---|---|
| author | Jiho Choi Changhoon Jeon Ji Hwan Lee Jo Ung Jang Fu Shi Quan Kyungjin Lee Woojin Kim Sun Kwang Kim |
| author_facet | Jiho Choi Changhoon Jeon Ji Hwan Lee Jo Ung Jang Fu Shi Quan Kyungjin Lee Woojin Kim Sun Kwang Kim |
| author_sort | Jiho Choi |
| collection | DOAJ |
| description | Paclitaxel, a chemotherapy drug for solid tumors, induces peripheral painful neuropathy. Bee venom acupuncture (BVA) has been reported to have potent analgesic effects, which are known to be mediated by activation of spinal α-adrenergic receptor. Here, we investigated the effect of BVA on mechanical hyperalgesia and spinal neuronal hyperexcitation induced by paclitaxel. The role of spinal α-adrenergic receptor subtypes in the analgesic effect of BVA was also observed. Administration of paclitaxel (total 8 mg/kg, intraperitoneal) on four alternate days (days 0, 2, 4, and 6) induced significant mechanical hyperalgesic signs, measured using a von Frey filament. BVA (1 mg/kg, ST36) relieved this mechanical hyperalgesia for at least two hours, and suppressed the hyperexcitation in spinal wide dynamic range neurons evoked by press or pinch stimulation. Both melittin (0.5 mg/kg, ST36) and phospholipase A2 (0.12 mg/kg, ST36) were shown to play an important part in this analgesic effect of the BVA, as they significantly attenuated the pain. Intrathecal pretreatment with the α2-adrenergic receptor antagonist (idazoxan, 50 µg), but not α1-adrenergic receptor antagonist (prazosin, 30 µg), blocked the analgesic effect of BVA. These results suggest that BVA has potent suppressive effects against paclitaxel-induced neuropathic pain, which were mediated by spinal α2-adrenergic receptor. |
| first_indexed | 2024-04-14T02:02:33Z |
| format | Article |
| id | doaj.art-87edff25ff7747e2b1320e727f816dcb |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-14T02:02:33Z |
| publishDate | 2017-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-87edff25ff7747e2b1320e727f816dcb2022-12-22T02:18:48ZengMDPI AGToxins2072-66512017-10-0191135110.3390/toxins9110351toxins9110351Suppressive Effects of Bee Venom Acupuncture on Paclitaxel-Induced Neuropathic Pain in Rats: Mediation by Spinal α2-Adrenergic ReceptorJiho Choi0Changhoon Jeon1Ji Hwan Lee2Jo Ung Jang3Fu Shi Quan4Kyungjin Lee5Woojin Kim6Sun Kwang Kim7Department of Physiology, College of Korean Medicine, Kyung Hee University, 26 Kyungheedae-ro, Dongdamoon-gu, Seoul 02447, KoreaDepartment of Physiology, College of Korean Medicine, Kyung Hee University, 26 Kyungheedae-ro, Dongdamoon-gu, Seoul 02447, KoreaDepartment of Science in Korean Medicine, Graduate School, Kyung Hee University, 26 Kyungheedae-ro, Dongdamoon-gu, Seoul 02447, KoreaDepartment of East-West Medicine, Graduate School, Kyung Hee University, 26 Kyungheedae-ro, Dongdamoon-gu, Seoul 02447, KoreaDepartment of Medical Zoology, School of Medicine, Kyung Hee University, 26 Kyungheedae-ro, Dongdamoon-gu, Seoul 02447, KoreaDepartment of Herbology, College of Korean Medicine, Kyung Hee University, 26 Kyungheedae-ro, Dongdamoon-gu, Seoul 02447, KoreaDepartment of Physiology, College of Korean Medicine, Kyung Hee University, 26 Kyungheedae-ro, Dongdamoon-gu, Seoul 02447, KoreaDepartment of Physiology, College of Korean Medicine, Kyung Hee University, 26 Kyungheedae-ro, Dongdamoon-gu, Seoul 02447, KoreaPaclitaxel, a chemotherapy drug for solid tumors, induces peripheral painful neuropathy. Bee venom acupuncture (BVA) has been reported to have potent analgesic effects, which are known to be mediated by activation of spinal α-adrenergic receptor. Here, we investigated the effect of BVA on mechanical hyperalgesia and spinal neuronal hyperexcitation induced by paclitaxel. The role of spinal α-adrenergic receptor subtypes in the analgesic effect of BVA was also observed. Administration of paclitaxel (total 8 mg/kg, intraperitoneal) on four alternate days (days 0, 2, 4, and 6) induced significant mechanical hyperalgesic signs, measured using a von Frey filament. BVA (1 mg/kg, ST36) relieved this mechanical hyperalgesia for at least two hours, and suppressed the hyperexcitation in spinal wide dynamic range neurons evoked by press or pinch stimulation. Both melittin (0.5 mg/kg, ST36) and phospholipase A2 (0.12 mg/kg, ST36) were shown to play an important part in this analgesic effect of the BVA, as they significantly attenuated the pain. Intrathecal pretreatment with the α2-adrenergic receptor antagonist (idazoxan, 50 µg), but not α1-adrenergic receptor antagonist (prazosin, 30 µg), blocked the analgesic effect of BVA. These results suggest that BVA has potent suppressive effects against paclitaxel-induced neuropathic pain, which were mediated by spinal α2-adrenergic receptor.https://www.mdpi.com/2072-6651/9/11/351bee venom acupuncturechemotherapy-induced neuropathic painpaclitaxel |
| spellingShingle | Jiho Choi Changhoon Jeon Ji Hwan Lee Jo Ung Jang Fu Shi Quan Kyungjin Lee Woojin Kim Sun Kwang Kim Suppressive Effects of Bee Venom Acupuncture on Paclitaxel-Induced Neuropathic Pain in Rats: Mediation by Spinal α2-Adrenergic Receptor Toxins bee venom acupuncture chemotherapy-induced neuropathic pain paclitaxel |
| title | Suppressive Effects of Bee Venom Acupuncture on Paclitaxel-Induced Neuropathic Pain in Rats: Mediation by Spinal α2-Adrenergic Receptor |
| title_full | Suppressive Effects of Bee Venom Acupuncture on Paclitaxel-Induced Neuropathic Pain in Rats: Mediation by Spinal α2-Adrenergic Receptor |
| title_fullStr | Suppressive Effects of Bee Venom Acupuncture on Paclitaxel-Induced Neuropathic Pain in Rats: Mediation by Spinal α2-Adrenergic Receptor |
| title_full_unstemmed | Suppressive Effects of Bee Venom Acupuncture on Paclitaxel-Induced Neuropathic Pain in Rats: Mediation by Spinal α2-Adrenergic Receptor |
| title_short | Suppressive Effects of Bee Venom Acupuncture on Paclitaxel-Induced Neuropathic Pain in Rats: Mediation by Spinal α2-Adrenergic Receptor |
| title_sort | suppressive effects of bee venom acupuncture on paclitaxel induced neuropathic pain in rats mediation by spinal α2 adrenergic receptor |
| topic | bee venom acupuncture chemotherapy-induced neuropathic pain paclitaxel |
| url | https://www.mdpi.com/2072-6651/9/11/351 |
| work_keys_str_mv | AT jihochoi suppressiveeffectsofbeevenomacupunctureonpaclitaxelinducedneuropathicpaininratsmediationbyspinala2adrenergicreceptor AT changhoonjeon suppressiveeffectsofbeevenomacupunctureonpaclitaxelinducedneuropathicpaininratsmediationbyspinala2adrenergicreceptor AT jihwanlee suppressiveeffectsofbeevenomacupunctureonpaclitaxelinducedneuropathicpaininratsmediationbyspinala2adrenergicreceptor AT joungjang suppressiveeffectsofbeevenomacupunctureonpaclitaxelinducedneuropathicpaininratsmediationbyspinala2adrenergicreceptor AT fushiquan suppressiveeffectsofbeevenomacupunctureonpaclitaxelinducedneuropathicpaininratsmediationbyspinala2adrenergicreceptor AT kyungjinlee suppressiveeffectsofbeevenomacupunctureonpaclitaxelinducedneuropathicpaininratsmediationbyspinala2adrenergicreceptor AT woojinkim suppressiveeffectsofbeevenomacupunctureonpaclitaxelinducedneuropathicpaininratsmediationbyspinala2adrenergicreceptor AT sunkwangkim suppressiveeffectsofbeevenomacupunctureonpaclitaxelinducedneuropathicpaininratsmediationbyspinala2adrenergicreceptor |