Kinase Inhibitor VvBKI1 Interacts with Ascorbate Peroxidase VvAPX1 Promoting Plant Resistance to Oomycetes
Downy mildew caused by oomycete pathogen <i>Plasmopara viticola</i> is a devastating disease of grapevine. <i>P. viticola</i> secretes an array of RXLR effectors to enhance virulence. One of these effectors, PvRXLR131, has been reported to interact with grape (<i>Vitis...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-03-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/6/5106 |
| Summary: | Downy mildew caused by oomycete pathogen <i>Plasmopara viticola</i> is a devastating disease of grapevine. <i>P. viticola</i> secretes an array of RXLR effectors to enhance virulence. One of these effectors, PvRXLR131, has been reported to interact with grape (<i>Vitis vinifera</i>) BRI1 kinase inhibitor (VvBKI1). BKI1 is conserved in <i>Nicotiana benthamiana</i> and <i>Arabidopsis thaliana</i>. However, the role of VvBKI1 in plant immunity is unknown. Here, we found transient expression of <i>VvBKI1</i> in grapevine and <i>N. benthamiana</i> increased its resistance to <i>P. viticola</i> and <i>Phytophthora capsici</i>, respectively. Furthermore, ectopic expression of VvBKI1 in Arabidopsis can increase its resistance to downy mildew caused by <i>Hyaloperonospora arabidopsidis</i>. Further experiments revealed that VvBKI1 interacts with a cytoplasmic ascorbate peroxidase, VvAPX1, an ROS-scavenging protein. Transient expression of <i>VvAPX1</i> in grape and <i>N. benthamiana</i> promoted its resistance against <i>P. viticola</i>, and <i>P. capsici</i>. Moreover, <i>VvAPX1</i> transgenic Arabidopsis is more resistant to <i>H. arabidopsidis</i>. Furthermore, both VvBKI1 and VvAPX1 transgenic Arabidopsis showed an elevated ascorbate peroxidase activity and enhanced disease resistance. In summary, our findings suggest a positive correlation between APX activity and resistance to oomycetes and that this regulatory network is conserved in <i>V. vinifera</i>, <i>N. benthamiana</i>, and <i>A. thaliana</i>. |
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| ISSN: | 1661-6596 1422-0067 |