Genome-wide DNA methylation profile in the peripheral blood of cocaine and crack dependents
Objective: Cocaine use disorders (CUDs) represent a major public health problem in many countries. To better understand the interaction between the environmental modulations and phenotype, the aim of the present study was to investigate the DNA methylation pattern of CUD patients, who had concomitan...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Psiquiatria (ABP)
2019-05-01
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| Series: | Brazilian Journal of Psychiatry |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-44462019005008101&lng=en&tlng=en |
| _version_ | 1828766521949683712 |
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| author | Caroline Camilo Mariana Maschietto Henrique C. Vieira Ana C. Tahira Gisele R. Gouveia Ana C. Feio dos Santos André B. Negrão Marcelo Ribeiro Ronaldo Laranjeira Homero Vallada Helena Brentani |
| author_facet | Caroline Camilo Mariana Maschietto Henrique C. Vieira Ana C. Tahira Gisele R. Gouveia Ana C. Feio dos Santos André B. Negrão Marcelo Ribeiro Ronaldo Laranjeira Homero Vallada Helena Brentani |
| author_sort | Caroline Camilo |
| collection | DOAJ |
| description | Objective: Cocaine use disorders (CUDs) represent a major public health problem in many countries. To better understand the interaction between the environmental modulations and phenotype, the aim of the present study was to investigate the DNA methylation pattern of CUD patients, who had concomitant cocaine and crack dependence, and healthy controls. Methods: We studied DNA methylation profiles in the peripheral blood of 23 CUD patients and 24 healthy control subjects using the Illumina Infinium HumanMethylation450 BeadChip arrays. Results: Comparison between CUD patients and controls revealed 186 differentially methylated positions (DMPs; adjusted p-value [adjP] < 10-5) related to 152 genes, with a subset of CpGs confirmed by pyrosequencing. DNA methylation patterns discriminated CUD patients and control groups. A gene network approach showed that the EHMT1, EHMT2, MAPK1, MAPK3, MAP2K1, and HDAC5 genes, which are involved in transcription and chromatin regulation cellular signaling pathways, were also associated with cocaine dependence. Conclusion: The investigation of DNA methylation patterns may contribute to a better understanding of the biological mechanisms involved in CUD. |
| first_indexed | 2024-12-11T07:07:50Z |
| format | Article |
| id | doaj.art-87ef52be35434fdf91bd8251db71d98c |
| institution | Directory Open Access Journal |
| issn | 1809-452X |
| language | English |
| last_indexed | 2024-12-11T07:07:50Z |
| publishDate | 2019-05-01 |
| publisher | Associação Brasileira de Psiquiatria (ABP) |
| record_format | Article |
| series | Brazilian Journal of Psychiatry |
| spelling | doaj.art-87ef52be35434fdf91bd8251db71d98c2022-12-22T01:16:26ZengAssociação Brasileira de Psiquiatria (ABP)Brazilian Journal of Psychiatry1809-452X2019-05-01010.1590/1516-4446-2018-0092S1516-44462019005008101Genome-wide DNA methylation profile in the peripheral blood of cocaine and crack dependentsCaroline CamiloMariana MaschiettoHenrique C. VieiraAna C. TahiraGisele R. GouveiaAna C. Feio dos SantosAndré B. NegrãoMarcelo RibeiroRonaldo LaranjeiraHomero ValladaHelena BrentaniObjective: Cocaine use disorders (CUDs) represent a major public health problem in many countries. To better understand the interaction between the environmental modulations and phenotype, the aim of the present study was to investigate the DNA methylation pattern of CUD patients, who had concomitant cocaine and crack dependence, and healthy controls. Methods: We studied DNA methylation profiles in the peripheral blood of 23 CUD patients and 24 healthy control subjects using the Illumina Infinium HumanMethylation450 BeadChip arrays. Results: Comparison between CUD patients and controls revealed 186 differentially methylated positions (DMPs; adjusted p-value [adjP] < 10-5) related to 152 genes, with a subset of CpGs confirmed by pyrosequencing. DNA methylation patterns discriminated CUD patients and control groups. A gene network approach showed that the EHMT1, EHMT2, MAPK1, MAPK3, MAP2K1, and HDAC5 genes, which are involved in transcription and chromatin regulation cellular signaling pathways, were also associated with cocaine dependence. Conclusion: The investigation of DNA methylation patterns may contribute to a better understanding of the biological mechanisms involved in CUD.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-44462019005008101&lng=en&tlng=enCocainecrack cocaineDNA methylationdrug dependenceepigenetics |
| spellingShingle | Caroline Camilo Mariana Maschietto Henrique C. Vieira Ana C. Tahira Gisele R. Gouveia Ana C. Feio dos Santos André B. Negrão Marcelo Ribeiro Ronaldo Laranjeira Homero Vallada Helena Brentani Genome-wide DNA methylation profile in the peripheral blood of cocaine and crack dependents Brazilian Journal of Psychiatry Cocaine crack cocaine DNA methylation drug dependence epigenetics |
| title | Genome-wide DNA methylation profile in the peripheral blood of cocaine and crack dependents |
| title_full | Genome-wide DNA methylation profile in the peripheral blood of cocaine and crack dependents |
| title_fullStr | Genome-wide DNA methylation profile in the peripheral blood of cocaine and crack dependents |
| title_full_unstemmed | Genome-wide DNA methylation profile in the peripheral blood of cocaine and crack dependents |
| title_short | Genome-wide DNA methylation profile in the peripheral blood of cocaine and crack dependents |
| title_sort | genome wide dna methylation profile in the peripheral blood of cocaine and crack dependents |
| topic | Cocaine crack cocaine DNA methylation drug dependence epigenetics |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-44462019005008101&lng=en&tlng=en |
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