First detection of white spot syndrome virus (WSSV) in wild mud crab Scylla spp. (de Haan, 1883) from Setiu Wetlands, Malaysia
In this study, tissue samples from 90 wild mud crabs (Scylla spp. including S. olivacea, S. Tranquebarica, and S. paramamosain) were collected during pre-monsoon, monsoon, and post-monsoon in the Setiu Wetlands, Terengganu, Malaysia. The tissue samples were screened for the presence of white spot...
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| Format: | Article |
| Language: | English |
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Prince of Songkla University
2019-02-01
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| Series: | Songklanakarin Journal of Science and Technology (SJST) |
| Subjects: | |
| Online Access: | https://rdo.psu.ac.th/sjstweb/journal/41-1/6.pdf |
| _version_ | 1828532282207502336 |
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| author | Nurshafiqah Norizan Faizah Shaharom-Harrison Najiah Musa Nadirah Musa Muhd Effendy Abd Wahid Sandra Catherine Zainathan |
| author_facet | Nurshafiqah Norizan Faizah Shaharom-Harrison Najiah Musa Nadirah Musa Muhd Effendy Abd Wahid Sandra Catherine Zainathan |
| author_sort | Nurshafiqah Norizan |
| collection | DOAJ |
| description | In this study, tissue samples from 90 wild mud crabs (Scylla spp. including S. olivacea, S. Tranquebarica, and S.
paramamosain) were collected during pre-monsoon, monsoon, and post-monsoon in the Setiu Wetlands, Terengganu, Malaysia.
The tissue samples were screened for the presence of white spot syndrome virus (WSSV) by PCR. This study was conducted to
detect the presence or absence of WSSV in wild Scylla spp. from the Setiu Wetlands at different times of sampling. WSSV DNA
was detected in 36% of the mud crabs. The DNA sequence of a 941 bp genome region amplified from a crab by PCR was
identified to be most similar (99% nucleotide sequence, 98% amino acid sequence) to a WSSV strain detected in Mexico
(KU216744.1) and Taiwan WSSV 419 strain (AY850066.1). The data indicated that mud crabs in the Setiu Wetlands might act
as a WSSV reservoir of risk to shrimp aquaculture. Our findings are the first detection of WSSV from wild mud crabs, Scylla
spp. in the Setiu Wetlands, Terengganu, Malaysia. |
| first_indexed | 2024-12-11T22:51:04Z |
| format | Article |
| id | doaj.art-87f6a7b2b27b4f4fbe1fae718804c1d0 |
| institution | Directory Open Access Journal |
| issn | 0125-3395 |
| language | English |
| last_indexed | 2024-12-11T22:51:04Z |
| publishDate | 2019-02-01 |
| publisher | Prince of Songkla University |
| record_format | Article |
| series | Songklanakarin Journal of Science and Technology (SJST) |
| spelling | doaj.art-87f6a7b2b27b4f4fbe1fae718804c1d02022-12-22T00:47:27ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952019-02-01411455210.14456/sjst-psu.2019.6First detection of white spot syndrome virus (WSSV) in wild mud crab Scylla spp. (de Haan, 1883) from Setiu Wetlands, MalaysiaNurshafiqah Norizan0Faizah Shaharom-Harrison1Najiah Musa2Nadirah Musa3Muhd Effendy Abd Wahid4Sandra Catherine Zainathan5School of Fisheries and Aquaculture Sciences, Universiti Malaysia Terengganu, Terengganu, 21030 MalaysiaSchool of Fisheries and Aquaculture Sciences, Universiti Malaysia Terengganu, Terengganu, 21030 MalaysiaSchool of Fisheries and Aquaculture Sciences, Universiti Malaysia Terengganu, Terengganu, 21030 MalaysiaSchool of Fisheries and Aquaculture Sciences, Universiti Malaysia Terengganu, Terengganu, 21030 MalaysiaSchool of Fisheries and Aquaculture Sciences, Universiti Malaysia Terengganu, Terengganu, 21030 MalaysiaSchool of Fisheries and Aquaculture Sciences, Universiti Malaysia Terengganu, Terengganu, 21030 MalaysiaIn this study, tissue samples from 90 wild mud crabs (Scylla spp. including S. olivacea, S. Tranquebarica, and S. paramamosain) were collected during pre-monsoon, monsoon, and post-monsoon in the Setiu Wetlands, Terengganu, Malaysia. The tissue samples were screened for the presence of white spot syndrome virus (WSSV) by PCR. This study was conducted to detect the presence or absence of WSSV in wild Scylla spp. from the Setiu Wetlands at different times of sampling. WSSV DNA was detected in 36% of the mud crabs. The DNA sequence of a 941 bp genome region amplified from a crab by PCR was identified to be most similar (99% nucleotide sequence, 98% amino acid sequence) to a WSSV strain detected in Mexico (KU216744.1) and Taiwan WSSV 419 strain (AY850066.1). The data indicated that mud crabs in the Setiu Wetlands might act as a WSSV reservoir of risk to shrimp aquaculture. Our findings are the first detection of WSSV from wild mud crabs, Scylla spp. in the Setiu Wetlands, Terengganu, Malaysia.https://rdo.psu.ac.th/sjstweb/journal/41-1/6.pdfmud crabScylla spp.white spot syndrome virusPCRSetiu Wetlands |
| spellingShingle | Nurshafiqah Norizan Faizah Shaharom-Harrison Najiah Musa Nadirah Musa Muhd Effendy Abd Wahid Sandra Catherine Zainathan First detection of white spot syndrome virus (WSSV) in wild mud crab Scylla spp. (de Haan, 1883) from Setiu Wetlands, Malaysia Songklanakarin Journal of Science and Technology (SJST) mud crab Scylla spp. white spot syndrome virus PCR Setiu Wetlands |
| title | First detection of white spot syndrome virus (WSSV) in wild mud crab Scylla spp. (de Haan, 1883) from Setiu Wetlands, Malaysia |
| title_full | First detection of white spot syndrome virus (WSSV) in wild mud crab Scylla spp. (de Haan, 1883) from Setiu Wetlands, Malaysia |
| title_fullStr | First detection of white spot syndrome virus (WSSV) in wild mud crab Scylla spp. (de Haan, 1883) from Setiu Wetlands, Malaysia |
| title_full_unstemmed | First detection of white spot syndrome virus (WSSV) in wild mud crab Scylla spp. (de Haan, 1883) from Setiu Wetlands, Malaysia |
| title_short | First detection of white spot syndrome virus (WSSV) in wild mud crab Scylla spp. (de Haan, 1883) from Setiu Wetlands, Malaysia |
| title_sort | first detection of white spot syndrome virus wssv in wild mud crab scylla spp de haan 1883 from setiu wetlands malaysia |
| topic | mud crab Scylla spp. white spot syndrome virus PCR Setiu Wetlands |
| url | https://rdo.psu.ac.th/sjstweb/journal/41-1/6.pdf |
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