Gene Expression Is Not Systematically Linked to Phytoalexin Production During Alfalfa Leaf Interaction with Pathogenic Bacteria
During an incompatible interaction between alfalfa leaves and Pseudomonas syringae pv. pisi, flavonoids accumulated between 6 and 24 h, whereas they could not be detected during the first 96 h of a compatible interaction with Xanthomonas campestris pv. alfalfae. Three flavonoids accumulated which we...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
1997-03-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.1997.10.2.257 |
| _version_ | 1811290209803829248 |
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| author | Christophe Sallaud Jose Zuanazzi Joumana El-Turk Juliette Leymarie Colette Breda Dominique Buffard Isabelle de Kozak Pascal Ratet Philippe Husson Adam Kondorosi Robert Esnault |
| author_facet | Christophe Sallaud Jose Zuanazzi Joumana El-Turk Juliette Leymarie Colette Breda Dominique Buffard Isabelle de Kozak Pascal Ratet Philippe Husson Adam Kondorosi Robert Esnault |
| author_sort | Christophe Sallaud |
| collection | DOAJ |
| description | During an incompatible interaction between alfalfa leaves and Pseudomonas syringae pv. pisi, flavonoids accumulated between 6 and 24 h, whereas they could not be detected during the first 96 h of a compatible interaction with Xanthomonas campestris pv. alfalfae. Three flavonoids accumulated which were identified as 4′,7-dihydroxyflavanone and 4′,7-dihydroxyflavone and 2′,4,4′-trihydroxychalcone. Surprisingly, the phytoalexin medicarpin was found only at a very low level. Analysis of both the infected and noninfected zones revealed that these flavonoids were detectable only in the infiltrated tissue. Northern hybridizations showed that transcripts encoding for chalcone synthase (CHS), chalcone reductase, chalcone isomerase, and isoflavone reductase (IFR) accumulated in both infiltrated and noninfiltrated zones. Measurements of the CHS and IFR activities in the infiltrated and noninfiltrated zones indicated that the levels of CHS activity were highly increased only in the infiltrated zones, whereas the levels of IFR were very slightly stimulated. These results suggested that an apparently coordinated expression of genes, involved in both the early and late steps of isoflavonoid biosynthesis, is not a sufficient condition for phytoalexin accumulation and that the fundamental regulatory steps might act at the post-transcriptional level. |
| first_indexed | 2024-04-13T04:08:57Z |
| format | Article |
| id | doaj.art-87f833cfc16c45eaacf06caa3829be4e |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T04:08:57Z |
| publishDate | 1997-03-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-87f833cfc16c45eaacf06caa3829be4e2022-12-22T03:03:10ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77061997-03-0110225726710.1094/MPMI.1997.10.2.257Gene Expression Is Not Systematically Linked to Phytoalexin Production During Alfalfa Leaf Interaction with Pathogenic BacteriaChristophe SallaudJose ZuanazziJoumana El-TurkJuliette LeymarieColette BredaDominique BuffardIsabelle de KozakPascal RatetPhilippe HussonAdam KondorosiRobert EsnaultDuring an incompatible interaction between alfalfa leaves and Pseudomonas syringae pv. pisi, flavonoids accumulated between 6 and 24 h, whereas they could not be detected during the first 96 h of a compatible interaction with Xanthomonas campestris pv. alfalfae. Three flavonoids accumulated which were identified as 4′,7-dihydroxyflavanone and 4′,7-dihydroxyflavone and 2′,4,4′-trihydroxychalcone. Surprisingly, the phytoalexin medicarpin was found only at a very low level. Analysis of both the infected and noninfected zones revealed that these flavonoids were detectable only in the infiltrated tissue. Northern hybridizations showed that transcripts encoding for chalcone synthase (CHS), chalcone reductase, chalcone isomerase, and isoflavone reductase (IFR) accumulated in both infiltrated and noninfiltrated zones. Measurements of the CHS and IFR activities in the infiltrated and noninfiltrated zones indicated that the levels of CHS activity were highly increased only in the infiltrated zones, whereas the levels of IFR were very slightly stimulated. These results suggested that an apparently coordinated expression of genes, involved in both the early and late steps of isoflavonoid biosynthesis, is not a sufficient condition for phytoalexin accumulation and that the fundamental regulatory steps might act at the post-transcriptional level.https://apsjournals.apsnet.org/doi/10.1094/MPMI.1997.10.2.257CHS and IFR activityflavonoid biosynthesisincompatible/compatible interactioninfiltrated/noninfiltrated tissues |
| spellingShingle | Christophe Sallaud Jose Zuanazzi Joumana El-Turk Juliette Leymarie Colette Breda Dominique Buffard Isabelle de Kozak Pascal Ratet Philippe Husson Adam Kondorosi Robert Esnault Gene Expression Is Not Systematically Linked to Phytoalexin Production During Alfalfa Leaf Interaction with Pathogenic Bacteria Molecular Plant-Microbe Interactions CHS and IFR activity flavonoid biosynthesis incompatible/compatible interaction infiltrated/noninfiltrated tissues |
| title | Gene Expression Is Not Systematically Linked to Phytoalexin Production During Alfalfa Leaf Interaction with Pathogenic Bacteria |
| title_full | Gene Expression Is Not Systematically Linked to Phytoalexin Production During Alfalfa Leaf Interaction with Pathogenic Bacteria |
| title_fullStr | Gene Expression Is Not Systematically Linked to Phytoalexin Production During Alfalfa Leaf Interaction with Pathogenic Bacteria |
| title_full_unstemmed | Gene Expression Is Not Systematically Linked to Phytoalexin Production During Alfalfa Leaf Interaction with Pathogenic Bacteria |
| title_short | Gene Expression Is Not Systematically Linked to Phytoalexin Production During Alfalfa Leaf Interaction with Pathogenic Bacteria |
| title_sort | gene expression is not systematically linked to phytoalexin production during alfalfa leaf interaction with pathogenic bacteria |
| topic | CHS and IFR activity flavonoid biosynthesis incompatible/compatible interaction infiltrated/noninfiltrated tissues |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.1997.10.2.257 |
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