A genome-wide integrated analysis of lncRNA-mRNA in melanocytes from white and brown skin hair boer goats (Capra aegagrus hircus)
Long noncoding RNAs (lncRNAs) are involved in many biological processes and have been extensively researched. Nonetheless, literature focusing on the roles of lncRNA in melanocytes is limited. Melanocytes are located in the basal layer of the epidermis and determine the color of an animal's ski...
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2022.1009174/full |
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| author | Ji Kai-yuan Ji Kai-yuan Zhao Yi-Wei Wen Ru-jun Ibrar Muhammad Khan Ibrar Muhammad Khan Zhang Yun-hai Zhang Yun-hai |
| author_facet | Ji Kai-yuan Ji Kai-yuan Zhao Yi-Wei Wen Ru-jun Ibrar Muhammad Khan Ibrar Muhammad Khan Zhang Yun-hai Zhang Yun-hai |
| author_sort | Ji Kai-yuan |
| collection | DOAJ |
| description | Long noncoding RNAs (lncRNAs) are involved in many biological processes and have been extensively researched. Nonetheless, literature focusing on the roles of lncRNA in melanocytes is limited. Melanocytes are located in the basal layer of the epidermis and determine the color of an animal's skin and hair by producing melanin. The mechanisms of melanogenesis remain unclear. Here, melanocytes from Boer goat skins were successfully isolated and verified using morphological observation, dopamine staining, silver ammonia staining, and immunohistochemical staining in vitro. Phenotypic testing revealed that melanocytes isolated from goat skins with white and brown hairs showed significant differences in proliferation, migration, and melanogenesis (**P < 0.01). RNA sequencing was performed with the isolated melanocytes, and through bioinformatic analysis, several candidate lncRNAs and mRNAs involved in stage-specific melanogenesis were identified. Functional enrichment analysis indicated that miRNA precursors and cis-regulatory effects of lncRNAs were deeply dissected using the function prediction software. Multiple lncRNA–mRNA networks were presumed to be involved in melanocyte migration, proliferation, and melanogenesis based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation. This research provided novel bioinformatic insights into the roles of lncRNAs in mammalian pigmentation. |
| first_indexed | 2024-04-12T14:15:24Z |
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| id | doaj.art-87fe9c5ae4c14c19a211e480f9fcc3f0 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-04-12T14:15:24Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Veterinary Science |
| spelling | doaj.art-87fe9c5ae4c14c19a211e480f9fcc3f02022-12-22T03:29:44ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692022-11-01910.3389/fvets.2022.10091741009174A genome-wide integrated analysis of lncRNA-mRNA in melanocytes from white and brown skin hair boer goats (Capra aegagrus hircus)Ji Kai-yuan0Ji Kai-yuan1Zhao Yi-Wei2Wen Ru-jun3Ibrar Muhammad Khan4Ibrar Muhammad Khan5Zhang Yun-hai6Zhang Yun-hai7Anhui Key Laboratory of Genetic Resources Protection and Biological Breeding for Livestock and Poultry, College of Animal Science and Technology, Anhui Agricultural University, Hefei, ChinaAnhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, College of Animal Science and Technology, Anhui Agricultural University, Hefei, ChinaAnhui Key Laboratory of Genetic Resources Protection and Biological Breeding for Livestock and Poultry, College of Animal Science and Technology, Anhui Agricultural University, Hefei, ChinaAnhui Key Laboratory of Genetic Resources Protection and Biological Breeding for Livestock and Poultry, College of Animal Science and Technology, Anhui Agricultural University, Hefei, ChinaAnhui Key Laboratory of Genetic Resources Protection and Biological Breeding for Livestock and Poultry, College of Animal Science and Technology, Anhui Agricultural University, Hefei, ChinaAnhui Province Key Laboratory of Embryo Development and Reproduction Regulation, Anhui Province Key Laboratory of Environmental Hormone and Reproduction, School of Biological and Food Engineering, Fuyang Normal University, Fuyang, ChinaAnhui Key Laboratory of Genetic Resources Protection and Biological Breeding for Livestock and Poultry, College of Animal Science and Technology, Anhui Agricultural University, Hefei, ChinaLinquan Comprehensive Experimental Station of Anhui Agricultural University, Anhui Agricultural University, Linquan, ChinaLong noncoding RNAs (lncRNAs) are involved in many biological processes and have been extensively researched. Nonetheless, literature focusing on the roles of lncRNA in melanocytes is limited. Melanocytes are located in the basal layer of the epidermis and determine the color of an animal's skin and hair by producing melanin. The mechanisms of melanogenesis remain unclear. Here, melanocytes from Boer goat skins were successfully isolated and verified using morphological observation, dopamine staining, silver ammonia staining, and immunohistochemical staining in vitro. Phenotypic testing revealed that melanocytes isolated from goat skins with white and brown hairs showed significant differences in proliferation, migration, and melanogenesis (**P < 0.01). RNA sequencing was performed with the isolated melanocytes, and through bioinformatic analysis, several candidate lncRNAs and mRNAs involved in stage-specific melanogenesis were identified. Functional enrichment analysis indicated that miRNA precursors and cis-regulatory effects of lncRNAs were deeply dissected using the function prediction software. Multiple lncRNA–mRNA networks were presumed to be involved in melanocyte migration, proliferation, and melanogenesis based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation. This research provided novel bioinformatic insights into the roles of lncRNAs in mammalian pigmentation.https://www.frontiersin.org/articles/10.3389/fvets.2022.1009174/fulllncRNAmelanocyteCapra hircusgenome-wide analysismRNA |
| spellingShingle | Ji Kai-yuan Ji Kai-yuan Zhao Yi-Wei Wen Ru-jun Ibrar Muhammad Khan Ibrar Muhammad Khan Zhang Yun-hai Zhang Yun-hai A genome-wide integrated analysis of lncRNA-mRNA in melanocytes from white and brown skin hair boer goats (Capra aegagrus hircus) Frontiers in Veterinary Science lncRNA melanocyte Capra hircus genome-wide analysis mRNA |
| title | A genome-wide integrated analysis of lncRNA-mRNA in melanocytes from white and brown skin hair boer goats (Capra aegagrus hircus) |
| title_full | A genome-wide integrated analysis of lncRNA-mRNA in melanocytes from white and brown skin hair boer goats (Capra aegagrus hircus) |
| title_fullStr | A genome-wide integrated analysis of lncRNA-mRNA in melanocytes from white and brown skin hair boer goats (Capra aegagrus hircus) |
| title_full_unstemmed | A genome-wide integrated analysis of lncRNA-mRNA in melanocytes from white and brown skin hair boer goats (Capra aegagrus hircus) |
| title_short | A genome-wide integrated analysis of lncRNA-mRNA in melanocytes from white and brown skin hair boer goats (Capra aegagrus hircus) |
| title_sort | genome wide integrated analysis of lncrna mrna in melanocytes from white and brown skin hair boer goats capra aegagrus hircus |
| topic | lncRNA melanocyte Capra hircus genome-wide analysis mRNA |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2022.1009174/full |
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