Transcriptome profiling reveals the anti-diabetic molecular mechanism of Cyclocarya paliurus polysaccharides
Cyclocarya paliurus polysaccharides have been reported to prevent diabetes, but the underlying mechanisms are unclear. This current study aimed to reveal the possible underlying anti-diabetic mechanisms of CPP based on transcriptome profiling. Our experimental results demonstrated CPP could protect...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019-04-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464618306789 |
| _version_ | 1818871358543626240 |
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| author | Jing Li Mei Luo Zhen Luo An-Yuan Guo Xiangliang Yang Minghua Hu Qiong Zhang Yanhong Zhu |
| author_facet | Jing Li Mei Luo Zhen Luo An-Yuan Guo Xiangliang Yang Minghua Hu Qiong Zhang Yanhong Zhu |
| author_sort | Jing Li |
| collection | DOAJ |
| description | Cyclocarya paliurus polysaccharides have been reported to prevent diabetes, but the underlying mechanisms are unclear. This current study aimed to reveal the possible underlying anti-diabetic mechanisms of CPP based on transcriptome profiling. Our experimental results demonstrated CPP could protect pancreas islets through decreasing oxidative stress and pro-inflammatory cytokines, and alleviate dyslipidemia, hepatic steatosis and liver injury. Pancreatic transcriptome profiling suggested CPP could down-regulate genes related to mitochondrion and fatty acid metabolism process, which decreased the production of reactive oxygen species and alleviated oxidative stress damage. Besides, liver transcriptome analysis indicated CPP down-regulated biological processes related to lipid metabolic, oxidation-reduction and apoptosis, and up-regulated protein synthesis, which contributed to preventing liver injuries. Additionally, the miR-199a-5p/miR-31a-5p and TF Jun may form regulatory modules to contribute to alleviating liver injuries. Taken together, our findings revealed the anti-diabetic effects of CPP and revealed potential molecular mechanisms at the transcriptional and post-transcriptional levels. |
| first_indexed | 2024-12-19T12:21:39Z |
| format | Article |
| id | doaj.art-88040ca164f64676af407394b958c6d6 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-19T12:21:39Z |
| publishDate | 2019-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-88040ca164f64676af407394b958c6d62022-12-21T20:21:45ZengElsevierJournal of Functional Foods1756-46462019-04-015518Transcriptome profiling reveals the anti-diabetic molecular mechanism of Cyclocarya paliurus polysaccharidesJing Li0Mei Luo1Zhen Luo2An-Yuan Guo3Xiangliang Yang4Minghua Hu5Qiong Zhang6Yanhong Zhu7National Engineering Research Center for Nano Medicine, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Bioinformatics and Systems Biology, Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, ChinaJoint Laboratory for the Research of Pharmaceutics, Huazhong University of Science and Technology and Infinitus, Wuhan, China; School of Food Science and Engineering, South China University of Technology, Guangzhou, ChinaDepartment of Bioinformatics and Systems Biology, Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, ChinaNational Engineering Research Center for Nano Medicine, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, ChinaJoint Laboratory for the Research of Pharmaceutics, Huazhong University of Science and Technology and Infinitus, Wuhan, ChinaDepartment of Bioinformatics and Systems Biology, Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China; Corresponding authors.National Engineering Research Center for Nano Medicine, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China; Corresponding authors.Cyclocarya paliurus polysaccharides have been reported to prevent diabetes, but the underlying mechanisms are unclear. This current study aimed to reveal the possible underlying anti-diabetic mechanisms of CPP based on transcriptome profiling. Our experimental results demonstrated CPP could protect pancreas islets through decreasing oxidative stress and pro-inflammatory cytokines, and alleviate dyslipidemia, hepatic steatosis and liver injury. Pancreatic transcriptome profiling suggested CPP could down-regulate genes related to mitochondrion and fatty acid metabolism process, which decreased the production of reactive oxygen species and alleviated oxidative stress damage. Besides, liver transcriptome analysis indicated CPP down-regulated biological processes related to lipid metabolic, oxidation-reduction and apoptosis, and up-regulated protein synthesis, which contributed to preventing liver injuries. Additionally, the miR-199a-5p/miR-31a-5p and TF Jun may form regulatory modules to contribute to alleviating liver injuries. Taken together, our findings revealed the anti-diabetic effects of CPP and revealed potential molecular mechanisms at the transcriptional and post-transcriptional levels.http://www.sciencedirect.com/science/article/pii/S1756464618306789DiabetesCyclocarya paliurus polysaccharidesTranscriptome profilingPancreas isletHepatic steatosis |
| spellingShingle | Jing Li Mei Luo Zhen Luo An-Yuan Guo Xiangliang Yang Minghua Hu Qiong Zhang Yanhong Zhu Transcriptome profiling reveals the anti-diabetic molecular mechanism of Cyclocarya paliurus polysaccharides Journal of Functional Foods Diabetes Cyclocarya paliurus polysaccharides Transcriptome profiling Pancreas islet Hepatic steatosis |
| title | Transcriptome profiling reveals the anti-diabetic molecular mechanism of Cyclocarya paliurus polysaccharides |
| title_full | Transcriptome profiling reveals the anti-diabetic molecular mechanism of Cyclocarya paliurus polysaccharides |
| title_fullStr | Transcriptome profiling reveals the anti-diabetic molecular mechanism of Cyclocarya paliurus polysaccharides |
| title_full_unstemmed | Transcriptome profiling reveals the anti-diabetic molecular mechanism of Cyclocarya paliurus polysaccharides |
| title_short | Transcriptome profiling reveals the anti-diabetic molecular mechanism of Cyclocarya paliurus polysaccharides |
| title_sort | transcriptome profiling reveals the anti diabetic molecular mechanism of cyclocarya paliurus polysaccharides |
| topic | Diabetes Cyclocarya paliurus polysaccharides Transcriptome profiling Pancreas islet Hepatic steatosis |
| url | http://www.sciencedirect.com/science/article/pii/S1756464618306789 |
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