Regulation of gametogenesis and zoosporogenesis in Ulva linza (Chlorophyta): comparison with Ulva mutabilis and potential for laboratory culture
Green Ulvophyte macroalgae represent attractive model systems for understanding growth, development and evolution. They are untapped resources for food, fuel and high-value compounds, but can also form nuisance blooms. To fully analyse green seaweed morphogenesis, controlled laboratory-based culture...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-01-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00015/full |
| _version_ | 1811277204793851904 |
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| author | Eleanor Fay Vesty Ralf W Kessler Thomas eWichard Juliet Clare Coates |
| author_facet | Eleanor Fay Vesty Ralf W Kessler Thomas eWichard Juliet Clare Coates |
| author_sort | Eleanor Fay Vesty |
| collection | DOAJ |
| description | Green Ulvophyte macroalgae represent attractive model systems for understanding growth, development and evolution. They are untapped resources for food, fuel and high-value compounds, but can also form nuisance blooms. To fully analyse green seaweed morphogenesis, controlled laboratory-based culture of these organisms is required. To date, only a single Ulvophyte species, Ulva mutabilis Føyn, has been manipulated to complete its whole life cycle in laboratory culture and to grow continuously under axenic conditions. Such cultures are essential to address multiple key questions in Ulva development and in algal-bacterial interactions. Here we show that another Ulva species, U. linza, with a broad geographical distribution, has the potential to be grown in axenic culture similarly to U. mutabilis. Ulva linza can be reliably induced to sporulate (form gametes and zoospores) in the laboratory, by cutting the relevant thallus tissue into small pieces and removing extracellular inhibitors (sporulation and swarming inhibitors). The germ cells work as an ideal feed stock for standardized algae cultures.The requirement of U. linza for bacterial signals to induce its normal morphology (particularly of the rhizoids) appears to have a species-specific component. The axenic cultures of these two species pave the way for future comparative studies of algal-microbial interactions. |
| first_indexed | 2024-04-13T00:11:28Z |
| format | Article |
| id | doaj.art-8804b1f7803c4c87b9da037a9dd5ae23 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-13T00:11:28Z |
| publishDate | 2015-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-8804b1f7803c4c87b9da037a9dd5ae232022-12-22T03:11:04ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2015-01-01610.3389/fpls.2015.00015126163Regulation of gametogenesis and zoosporogenesis in Ulva linza (Chlorophyta): comparison with Ulva mutabilis and potential for laboratory cultureEleanor Fay Vesty0Ralf W Kessler1Thomas eWichard2Juliet Clare Coates3University of BirminghamFriedrich Schiller UniversityFriedrich Schiller UniversityUniversity of BirminghamGreen Ulvophyte macroalgae represent attractive model systems for understanding growth, development and evolution. They are untapped resources for food, fuel and high-value compounds, but can also form nuisance blooms. To fully analyse green seaweed morphogenesis, controlled laboratory-based culture of these organisms is required. To date, only a single Ulvophyte species, Ulva mutabilis Føyn, has been manipulated to complete its whole life cycle in laboratory culture and to grow continuously under axenic conditions. Such cultures are essential to address multiple key questions in Ulva development and in algal-bacterial interactions. Here we show that another Ulva species, U. linza, with a broad geographical distribution, has the potential to be grown in axenic culture similarly to U. mutabilis. Ulva linza can be reliably induced to sporulate (form gametes and zoospores) in the laboratory, by cutting the relevant thallus tissue into small pieces and removing extracellular inhibitors (sporulation and swarming inhibitors). The germ cells work as an ideal feed stock for standardized algae cultures.The requirement of U. linza for bacterial signals to induce its normal morphology (particularly of the rhizoids) appears to have a species-specific component. The axenic cultures of these two species pave the way for future comparative studies of algal-microbial interactions.http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00015/fullAxenic CultureCytophagaGametogenesisMorphogenesisRoseobactergreen algae |
| spellingShingle | Eleanor Fay Vesty Ralf W Kessler Thomas eWichard Juliet Clare Coates Regulation of gametogenesis and zoosporogenesis in Ulva linza (Chlorophyta): comparison with Ulva mutabilis and potential for laboratory culture Frontiers in Plant Science Axenic Culture Cytophaga Gametogenesis Morphogenesis Roseobacter green algae |
| title | Regulation of gametogenesis and zoosporogenesis in Ulva linza (Chlorophyta): comparison with Ulva mutabilis and potential for laboratory culture |
| title_full | Regulation of gametogenesis and zoosporogenesis in Ulva linza (Chlorophyta): comparison with Ulva mutabilis and potential for laboratory culture |
| title_fullStr | Regulation of gametogenesis and zoosporogenesis in Ulva linza (Chlorophyta): comparison with Ulva mutabilis and potential for laboratory culture |
| title_full_unstemmed | Regulation of gametogenesis and zoosporogenesis in Ulva linza (Chlorophyta): comparison with Ulva mutabilis and potential for laboratory culture |
| title_short | Regulation of gametogenesis and zoosporogenesis in Ulva linza (Chlorophyta): comparison with Ulva mutabilis and potential for laboratory culture |
| title_sort | regulation of gametogenesis and zoosporogenesis in ulva linza chlorophyta comparison with ulva mutabilis and potential for laboratory culture |
| topic | Axenic Culture Cytophaga Gametogenesis Morphogenesis Roseobacter green algae |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00015/full |
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