Molecular Detection of Virulence Factors of Enterococcus Faecalis Isolated From Urine Samples in Duhok City, Kurdistan Region/Iraq.
Enterococcus faecalis is one of the leading causes of many infections and mainly urinary tract infections. This pathogen developed high resistance to multiple antibiotics and it harbor many virulence factors genes. This study aimed to determine the antibiotic resistance patterns and screening for so...
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| Format: | Article |
| Language: | English |
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University of Zakho
2016-06-01
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| Series: | Science Journal of University of Zakho |
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| Online Access: | https://sjuoz.uoz.edu.krd/index.php/sjuoz/article/view/306 |
| _version_ | 1818677943192256512 |
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| author | Haval M. Khalid |
| author_facet | Haval M. Khalid |
| author_sort | Haval M. Khalid |
| collection | DOAJ |
| description | Enterococcus faecalis is one of the leading causes of many infections and mainly urinary tract infections. This pathogen developed high resistance to multiple antibiotics and it harbor many virulence factors genes. This study aimed to determine the antibiotic resistance patterns and screening for some virulence factor genes of E. faecalis isolated from urinary tract infection. Urine samples were collected from 788 outpatient’s clinic having clinical signs of UTI that visited Azadi Teaching Hospital in Duhok city. Urine samples were cultured on bacteriological media and isolated colonies identified using standard bacteriological methods. Antibiotic susceptibility was performed by Kirby Bauer test. All isolates were subjected to species-specific PCR assay for confirmatory identification followed by targeting virulence genes. Twenty five isolates of E. faecalis were detected and confirmed by species-specific PCR assay that expressed high antibiotic-resistance to many selected drugs except norfloxacin, penicillin and ampicillin. The most prevalent genes among all isolates were cpd genes followed by asa1, ace, esp, and gelE. Bearing of virulence genes combination were more frequent among multiple-antibiotic resistant strains. This study highlighted on E. faecalis as causes of UTI in Duhok city that showed multiple resistances to common antibiotics and harboring more than one virulence gene. |
| first_indexed | 2024-12-17T09:07:24Z |
| format | Article |
| id | doaj.art-88056a05bdb74c7291ac1df1de831768 |
| institution | Directory Open Access Journal |
| issn | 2663-628X 2663-6298 |
| language | English |
| last_indexed | 2024-12-17T09:07:24Z |
| publishDate | 2016-06-01 |
| publisher | University of Zakho |
| record_format | Article |
| series | Science Journal of University of Zakho |
| spelling | doaj.art-88056a05bdb74c7291ac1df1de8317682022-12-21T21:55:24ZengUniversity of ZakhoScience Journal of University of Zakho2663-628X2663-62982016-06-01416372306Molecular Detection of Virulence Factors of Enterococcus Faecalis Isolated From Urine Samples in Duhok City, Kurdistan Region/Iraq.Haval M. Khalid0University of ZakhoEnterococcus faecalis is one of the leading causes of many infections and mainly urinary tract infections. This pathogen developed high resistance to multiple antibiotics and it harbor many virulence factors genes. This study aimed to determine the antibiotic resistance patterns and screening for some virulence factor genes of E. faecalis isolated from urinary tract infection. Urine samples were collected from 788 outpatient’s clinic having clinical signs of UTI that visited Azadi Teaching Hospital in Duhok city. Urine samples were cultured on bacteriological media and isolated colonies identified using standard bacteriological methods. Antibiotic susceptibility was performed by Kirby Bauer test. All isolates were subjected to species-specific PCR assay for confirmatory identification followed by targeting virulence genes. Twenty five isolates of E. faecalis were detected and confirmed by species-specific PCR assay that expressed high antibiotic-resistance to many selected drugs except norfloxacin, penicillin and ampicillin. The most prevalent genes among all isolates were cpd genes followed by asa1, ace, esp, and gelE. Bearing of virulence genes combination were more frequent among multiple-antibiotic resistant strains. This study highlighted on E. faecalis as causes of UTI in Duhok city that showed multiple resistances to common antibiotics and harboring more than one virulence gene.https://sjuoz.uoz.edu.krd/index.php/sjuoz/article/view/306Enterococcus faecalisUrinary tract infectionPCRVirulence genes |
| spellingShingle | Haval M. Khalid Molecular Detection of Virulence Factors of Enterococcus Faecalis Isolated From Urine Samples in Duhok City, Kurdistan Region/Iraq. Science Journal of University of Zakho Enterococcus faecalis Urinary tract infection PCR Virulence genes |
| title | Molecular Detection of Virulence Factors of Enterococcus Faecalis Isolated From Urine Samples in Duhok City, Kurdistan Region/Iraq. |
| title_full | Molecular Detection of Virulence Factors of Enterococcus Faecalis Isolated From Urine Samples in Duhok City, Kurdistan Region/Iraq. |
| title_fullStr | Molecular Detection of Virulence Factors of Enterococcus Faecalis Isolated From Urine Samples in Duhok City, Kurdistan Region/Iraq. |
| title_full_unstemmed | Molecular Detection of Virulence Factors of Enterococcus Faecalis Isolated From Urine Samples in Duhok City, Kurdistan Region/Iraq. |
| title_short | Molecular Detection of Virulence Factors of Enterococcus Faecalis Isolated From Urine Samples in Duhok City, Kurdistan Region/Iraq. |
| title_sort | molecular detection of virulence factors of enterococcus faecalis isolated from urine samples in duhok city kurdistan region iraq |
| topic | Enterococcus faecalis Urinary tract infection PCR Virulence genes |
| url | https://sjuoz.uoz.edu.krd/index.php/sjuoz/article/view/306 |
| work_keys_str_mv | AT havalmkhalid moleculardetectionofvirulencefactorsofenterococcusfaecalisisolatedfromurinesamplesinduhokcitykurdistanregioniraq |