Transcriptome analysis of the transition from primary to secondary growth of vertical stem in Eucalyptus grandis
Abstract Eucalyptus was one of the most cultivated hardwood species worldwide, with rapid growth, good wood properties and a wide range of adaptability. Eucalyptus stem undergoes primary growth (longitudinal growth) followed by secondary growth (radial growth), which produces biomass that is an impo...
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| Materiálatiipa: | Artihkal |
| Giella: | English |
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BMC
2024-02-01
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| Ráidu: | BMC Plant Biology |
| Fáttát: | |
| Liŋkkat: | https://doi.org/10.1186/s12870-024-04731-3 |
| _version_ | 1827328036082548736 |
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| author | Fangping Zhou Haonan Zhang Shanshan Chen Chunjie Fan |
| author_facet | Fangping Zhou Haonan Zhang Shanshan Chen Chunjie Fan |
| author_sort | Fangping Zhou |
| collection | DOAJ |
| description | Abstract Eucalyptus was one of the most cultivated hardwood species worldwide, with rapid growth, good wood properties and a wide range of adaptability. Eucalyptus stem undergoes primary growth (longitudinal growth) followed by secondary growth (radial growth), which produces biomass that is an important source of energy worldwide. In order to better understand the genetic regulation of secondary growth in Eucalyptus grandis, Transcriptome analyses in stem segments along a developmental gradient from the third internode to the eleventh internode of E. grandis that spanned primary to secondary growth were carried out. 5,149 genes that were differentially expressed during stem development were identified. Combining the trend analysis by the Mfuzz method and the module-trait correlation analysis by the Weighted Gene Co-expression Network Analysis method, a total of 70 differentially expressed genes (DEGs) selected from 868 DEGs with high connectivity were found to be closely correlated with secondary growth. Results revealed that the differential expression of these DEGs suggests that they may involve in the primary growth or secondary growth. AP1, YAB2 TFs and EXP genes are highly expressed in the IN3, whereas NAC, MYB TFs are likely to be important for secondary growth. These results will expand our understanding of the complex molecular and cellular events of secondary growth and provide a foundation for future studies on wood formation in Eucalyptus. |
| first_indexed | 2024-03-07T15:11:11Z |
| format | Article |
| id | doaj.art-8807fe8ceb984e1ba82088f4658ee677 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-03-07T15:11:11Z |
| publishDate | 2024-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-8807fe8ceb984e1ba82088f4658ee6772024-03-05T18:37:58ZengBMCBMC Plant Biology1471-22292024-02-0124111210.1186/s12870-024-04731-3Transcriptome analysis of the transition from primary to secondary growth of vertical stem in Eucalyptus grandisFangping Zhou0Haonan Zhang1Shanshan Chen2Chunjie Fan3State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of ForestryState Key Laboratory of Tree Genetics and Breeding, Chinese Academy of ForestryState Key Laboratory of Tree Genetics and Breeding, Chinese Academy of ForestryState Key Laboratory of Tree Genetics and Breeding, Chinese Academy of ForestryAbstract Eucalyptus was one of the most cultivated hardwood species worldwide, with rapid growth, good wood properties and a wide range of adaptability. Eucalyptus stem undergoes primary growth (longitudinal growth) followed by secondary growth (radial growth), which produces biomass that is an important source of energy worldwide. In order to better understand the genetic regulation of secondary growth in Eucalyptus grandis, Transcriptome analyses in stem segments along a developmental gradient from the third internode to the eleventh internode of E. grandis that spanned primary to secondary growth were carried out. 5,149 genes that were differentially expressed during stem development were identified. Combining the trend analysis by the Mfuzz method and the module-trait correlation analysis by the Weighted Gene Co-expression Network Analysis method, a total of 70 differentially expressed genes (DEGs) selected from 868 DEGs with high connectivity were found to be closely correlated with secondary growth. Results revealed that the differential expression of these DEGs suggests that they may involve in the primary growth or secondary growth. AP1, YAB2 TFs and EXP genes are highly expressed in the IN3, whereas NAC, MYB TFs are likely to be important for secondary growth. These results will expand our understanding of the complex molecular and cellular events of secondary growth and provide a foundation for future studies on wood formation in Eucalyptus.https://doi.org/10.1186/s12870-024-04731-3Eucalyptus grandisSecondary growthWood formationStem |
| spellingShingle | Fangping Zhou Haonan Zhang Shanshan Chen Chunjie Fan Transcriptome analysis of the transition from primary to secondary growth of vertical stem in Eucalyptus grandis BMC Plant Biology Eucalyptus grandis Secondary growth Wood formation Stem |
| title | Transcriptome analysis of the transition from primary to secondary growth of vertical stem in Eucalyptus grandis |
| title_full | Transcriptome analysis of the transition from primary to secondary growth of vertical stem in Eucalyptus grandis |
| title_fullStr | Transcriptome analysis of the transition from primary to secondary growth of vertical stem in Eucalyptus grandis |
| title_full_unstemmed | Transcriptome analysis of the transition from primary to secondary growth of vertical stem in Eucalyptus grandis |
| title_short | Transcriptome analysis of the transition from primary to secondary growth of vertical stem in Eucalyptus grandis |
| title_sort | transcriptome analysis of the transition from primary to secondary growth of vertical stem in eucalyptus grandis |
| topic | Eucalyptus grandis Secondary growth Wood formation Stem |
| url | https://doi.org/10.1186/s12870-024-04731-3 |
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