Analysis of salivary IgA, amylase, lactoferrin, and lysozyme before and after comprehensive dental treatment in children: A prospective study
Objective: This study aimed to evaluate the levels of salivary IgA, amylase, lactoferrin, and lysozyme before and after comprehensive dental treatment in children with early childhood caries. Design: Thirty children aged 36–60 months, with a deft score ≥5, were selected for the study. Before dental...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2017-01-01
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| Series: | Contemporary Clinical Dentistry |
| Subjects: | |
| Online Access: | http://www.contempclindent.org/article.asp?issn=0976-237X;year=2017;volume=8;issue=4;spage=526;epage=530;aulast=Sharma |
| _version_ | 1818452071766032384 |
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| author | Akhilesh Sharma Priya Subramaniam Shebin Moiden |
| author_facet | Akhilesh Sharma Priya Subramaniam Shebin Moiden |
| author_sort | Akhilesh Sharma |
| collection | DOAJ |
| description | Objective: This study aimed to evaluate the levels of salivary IgA, amylase, lactoferrin, and lysozyme before and after comprehensive dental treatment in children with early childhood caries. Design: Thirty children aged 36–60 months, with a deft score ≥5, were selected for the study. Before dental treatment, paraffin-stimulated whole saliva was collected in a sterile graduated cup for a period of 5 min. The saliva samples were quantitatively analyzed for levels of IgA, amylase, lactoferrin, and lysozyme using enzyme-linked immunosorbent assay. Comprehensive dental treatment was carried out in all the children including caries preventive procedures. A second sample of saliva was collected at 3 months following completion of dental treatment. Data obtained was subjected to statistical analysis using Student's t-test. Results: The mean levels of salivary IgA was significantly reduced from 59.60 μg/ml to 56.42 μg/ml after dental treatment (P < 0.05). There was a significant reduction in the levels of salivary amylase from 115.78 μg/ml to 113.33 μg/ml (P < 0.001). Following dental treatment, salivary lactoferrin and lysozyme levels were significantly reduced from 3.76 μg/ml and 10.62 μg/ml to 3.44 μg/ml and 10.27 μg/ml, respectively (P < 0.001). Conclusions: Levels of salivary IgA, amylase, lactoferrin, and lysozyme were reduced significantly at 3 months following comprehensive dental treatment. |
| first_indexed | 2024-12-14T21:17:16Z |
| format | Article |
| id | doaj.art-8808145c5ffb4458b430e5eac4705b60 |
| institution | Directory Open Access Journal |
| issn | 0976-237X 0976-2361 |
| language | English |
| last_indexed | 2024-12-14T21:17:16Z |
| publishDate | 2017-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Contemporary Clinical Dentistry |
| spelling | doaj.art-8808145c5ffb4458b430e5eac4705b602022-12-21T22:47:01ZengWolters Kluwer Medknow PublicationsContemporary Clinical Dentistry0976-237X0976-23612017-01-018452653010.4103/ccd.ccd_103_17Analysis of salivary IgA, amylase, lactoferrin, and lysozyme before and after comprehensive dental treatment in children: A prospective studyAkhilesh SharmaPriya SubramaniamShebin MoidenObjective: This study aimed to evaluate the levels of salivary IgA, amylase, lactoferrin, and lysozyme before and after comprehensive dental treatment in children with early childhood caries. Design: Thirty children aged 36–60 months, with a deft score ≥5, were selected for the study. Before dental treatment, paraffin-stimulated whole saliva was collected in a sterile graduated cup for a period of 5 min. The saliva samples were quantitatively analyzed for levels of IgA, amylase, lactoferrin, and lysozyme using enzyme-linked immunosorbent assay. Comprehensive dental treatment was carried out in all the children including caries preventive procedures. A second sample of saliva was collected at 3 months following completion of dental treatment. Data obtained was subjected to statistical analysis using Student's t-test. Results: The mean levels of salivary IgA was significantly reduced from 59.60 μg/ml to 56.42 μg/ml after dental treatment (P < 0.05). There was a significant reduction in the levels of salivary amylase from 115.78 μg/ml to 113.33 μg/ml (P < 0.001). Following dental treatment, salivary lactoferrin and lysozyme levels were significantly reduced from 3.76 μg/ml and 10.62 μg/ml to 3.44 μg/ml and 10.27 μg/ml, respectively (P < 0.001). Conclusions: Levels of salivary IgA, amylase, lactoferrin, and lysozyme were reduced significantly at 3 months following comprehensive dental treatment.http://www.contempclindent.org/article.asp?issn=0976-237X;year=2017;volume=8;issue=4;spage=526;epage=530;aulast=SharmaAmylasedental carieslactoferrinlysozymesalivary IgA |
| spellingShingle | Akhilesh Sharma Priya Subramaniam Shebin Moiden Analysis of salivary IgA, amylase, lactoferrin, and lysozyme before and after comprehensive dental treatment in children: A prospective study Contemporary Clinical Dentistry Amylase dental caries lactoferrin lysozyme salivary IgA |
| title | Analysis of salivary IgA, amylase, lactoferrin, and lysozyme before and after comprehensive dental treatment in children: A prospective study |
| title_full | Analysis of salivary IgA, amylase, lactoferrin, and lysozyme before and after comprehensive dental treatment in children: A prospective study |
| title_fullStr | Analysis of salivary IgA, amylase, lactoferrin, and lysozyme before and after comprehensive dental treatment in children: A prospective study |
| title_full_unstemmed | Analysis of salivary IgA, amylase, lactoferrin, and lysozyme before and after comprehensive dental treatment in children: A prospective study |
| title_short | Analysis of salivary IgA, amylase, lactoferrin, and lysozyme before and after comprehensive dental treatment in children: A prospective study |
| title_sort | analysis of salivary iga amylase lactoferrin and lysozyme before and after comprehensive dental treatment in children a prospective study |
| topic | Amylase dental caries lactoferrin lysozyme salivary IgA |
| url | http://www.contempclindent.org/article.asp?issn=0976-237X;year=2017;volume=8;issue=4;spage=526;epage=530;aulast=Sharma |
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