Pseudomonas Isolates as Potential Biofungicides of Green Mold (Penicillium Digitatum) on Orange Fruit
Green mold, caused by Penicillium digitatum, is a serious disease of citrus fruit during postharvest. The focus of this study was to evaluate possible biological alternatives to chemical fungicides for P. digitatum control. Four Pseudomonas isolates (Q110B, Q125B, Q112B, and Q004B) were isolated fro...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2022-12-01
|
| Series: | International Journal of Fruit Science |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/15538362.2021.2018671 |
| _version_ | 1818976024233246720 |
|---|---|
| author | Redouan Qessaoui Mariem Zanzan Abdelhadi Ajerrar Hind Lahmyed Ahmed Boumair Rachid Tahzima Mohamed Alouani El Hassan Mayad Bouchra Chebli Stuart Alan Walters Rachid Bouharroud |
| author_facet | Redouan Qessaoui Mariem Zanzan Abdelhadi Ajerrar Hind Lahmyed Ahmed Boumair Rachid Tahzima Mohamed Alouani El Hassan Mayad Bouchra Chebli Stuart Alan Walters Rachid Bouharroud |
| author_sort | Redouan Qessaoui |
| collection | DOAJ |
| description | Green mold, caused by Penicillium digitatum, is a serious disease of citrus fruit during postharvest. The focus of this study was to evaluate possible biological alternatives to chemical fungicides for P. digitatum control. Four Pseudomonas isolates (Q110B, Q125B, Q112B, and Q004B) were isolated from rhizospheric soil and evaluated for their antagonistic activity against P. digitatum. The in vitro evaluations of these isolates by dual culture revealed an important zone of inhibition after 3 days of incubation at 25 ± 2°C. The Q004B isolate provided a high zone of inhibition in vitro at 45 mm diameter. Four Pseudomonas isolates tested showed antagonistic activity against P. digitatum on orange fruit after 7 days of inoculation under postharvest conditions. Inhibition percentage of P. digitatum on orange fruit ranged from 22 to 85% for Q004B and Q112B, respectively. Regarding the mechanism of action, the four tested isolates produced volatile compounds in vitro with inhibition percentage ranging from 35% to 79%, respectively for Q004B andQ125B. The Q004B isolate caused a high spore inhibition rate of 46%, while Q110B had no effect on spore germination. The four isolates of Pseudomonas produced siderophores in CAS medium. Additionally, Q125B, Q112B, and Q004B showed a positive chitinolytic activity, whereas Q110B provided negative results to the chitinase assay. These isolates represent potential candidates for biological control of citrus green mold and may be effectively used as a sustainable alternative measure to chemical fungicide control. |
| first_indexed | 2024-12-20T16:05:16Z |
| format | Article |
| id | doaj.art-8808f4714c1f4ecb8a7cae6adb1237f6 |
| institution | Directory Open Access Journal |
| issn | 1553-8362 1553-8621 |
| language | English |
| last_indexed | 2024-12-20T16:05:16Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | International Journal of Fruit Science |
| spelling | doaj.art-8808f4714c1f4ecb8a7cae6adb1237f62022-12-21T19:34:10ZengTaylor & Francis GroupInternational Journal of Fruit Science1553-83621553-86212022-12-0122114215010.1080/15538362.2021.20186712018671Pseudomonas Isolates as Potential Biofungicides of Green Mold (Penicillium Digitatum) on Orange FruitRedouan Qessaoui0Mariem Zanzan1Abdelhadi Ajerrar2Hind Lahmyed3Ahmed Boumair4Rachid Tahzima5Mohamed Alouani6El Hassan Mayad7Bouchra Chebli8Stuart Alan Walters9Rachid Bouharroud10National Institute of Agricultural ResearchIbn Zohr UniversityNational Institute of Agricultural ResearchNational Institute of Agricultural ResearchNational Institute of Agricultural ResearchUniversity of Liège, Gembloux Agro-BioTechIbn Zohr UniversityIbn Zohr UniversityIbn Zohr UniversitySouthern Illinois UniversityNational Institute of Agricultural ResearchGreen mold, caused by Penicillium digitatum, is a serious disease of citrus fruit during postharvest. The focus of this study was to evaluate possible biological alternatives to chemical fungicides for P. digitatum control. Four Pseudomonas isolates (Q110B, Q125B, Q112B, and Q004B) were isolated from rhizospheric soil and evaluated for their antagonistic activity against P. digitatum. The in vitro evaluations of these isolates by dual culture revealed an important zone of inhibition after 3 days of incubation at 25 ± 2°C. The Q004B isolate provided a high zone of inhibition in vitro at 45 mm diameter. Four Pseudomonas isolates tested showed antagonistic activity against P. digitatum on orange fruit after 7 days of inoculation under postharvest conditions. Inhibition percentage of P. digitatum on orange fruit ranged from 22 to 85% for Q004B and Q112B, respectively. Regarding the mechanism of action, the four tested isolates produced volatile compounds in vitro with inhibition percentage ranging from 35% to 79%, respectively for Q004B andQ125B. The Q004B isolate caused a high spore inhibition rate of 46%, while Q110B had no effect on spore germination. The four isolates of Pseudomonas produced siderophores in CAS medium. Additionally, Q125B, Q112B, and Q004B showed a positive chitinolytic activity, whereas Q110B provided negative results to the chitinase assay. These isolates represent potential candidates for biological control of citrus green mold and may be effectively used as a sustainable alternative measure to chemical fungicide control.http://dx.doi.org/10.1080/15538362.2021.2018671pseudomonaspenicillium digitatumorange fruitbiocontrol |
| spellingShingle | Redouan Qessaoui Mariem Zanzan Abdelhadi Ajerrar Hind Lahmyed Ahmed Boumair Rachid Tahzima Mohamed Alouani El Hassan Mayad Bouchra Chebli Stuart Alan Walters Rachid Bouharroud Pseudomonas Isolates as Potential Biofungicides of Green Mold (Penicillium Digitatum) on Orange Fruit International Journal of Fruit Science pseudomonas penicillium digitatum orange fruit biocontrol |
| title | Pseudomonas Isolates as Potential Biofungicides of Green Mold (Penicillium Digitatum) on Orange Fruit |
| title_full | Pseudomonas Isolates as Potential Biofungicides of Green Mold (Penicillium Digitatum) on Orange Fruit |
| title_fullStr | Pseudomonas Isolates as Potential Biofungicides of Green Mold (Penicillium Digitatum) on Orange Fruit |
| title_full_unstemmed | Pseudomonas Isolates as Potential Biofungicides of Green Mold (Penicillium Digitatum) on Orange Fruit |
| title_short | Pseudomonas Isolates as Potential Biofungicides of Green Mold (Penicillium Digitatum) on Orange Fruit |
| title_sort | pseudomonas isolates as potential biofungicides of green mold penicillium digitatum on orange fruit |
| topic | pseudomonas penicillium digitatum orange fruit biocontrol |
| url | http://dx.doi.org/10.1080/15538362.2021.2018671 |
| work_keys_str_mv | AT redouanqessaoui pseudomonasisolatesaspotentialbiofungicidesofgreenmoldpenicilliumdigitatumonorangefruit AT mariemzanzan pseudomonasisolatesaspotentialbiofungicidesofgreenmoldpenicilliumdigitatumonorangefruit AT abdelhadiajerrar pseudomonasisolatesaspotentialbiofungicidesofgreenmoldpenicilliumdigitatumonorangefruit AT hindlahmyed pseudomonasisolatesaspotentialbiofungicidesofgreenmoldpenicilliumdigitatumonorangefruit AT ahmedboumair pseudomonasisolatesaspotentialbiofungicidesofgreenmoldpenicilliumdigitatumonorangefruit AT rachidtahzima pseudomonasisolatesaspotentialbiofungicidesofgreenmoldpenicilliumdigitatumonorangefruit AT mohamedalouani pseudomonasisolatesaspotentialbiofungicidesofgreenmoldpenicilliumdigitatumonorangefruit AT elhassanmayad pseudomonasisolatesaspotentialbiofungicidesofgreenmoldpenicilliumdigitatumonorangefruit AT bouchrachebli pseudomonasisolatesaspotentialbiofungicidesofgreenmoldpenicilliumdigitatumonorangefruit AT stuartalanwalters pseudomonasisolatesaspotentialbiofungicidesofgreenmoldpenicilliumdigitatumonorangefruit AT rachidbouharroud pseudomonasisolatesaspotentialbiofungicidesofgreenmoldpenicilliumdigitatumonorangefruit |