Annexin A5 regulates Leydig cell testosterone production via ERK1/2 pathway
This study was to investigate the effect of annexin A5 on testosterone secretion from primary rat Leydig cells and the underlying mechanisms. Isolated rat Leydig cells were treated with annexin A5. Testosterone production was detected by chemiluminescence assay. The protein and mRNA of Steroidogenic...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2016-01-01
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| Series: | Asian Journal of Andrology |
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| Online Access: | http://www.ajandrology.com/article.asp?issn=1008-682X;year=2016;volume=18;issue=3;spage=456;epage=461;aulast=He |
| _version_ | 1819046848512393216 |
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| author | Ze He Qin Sun Yuan-Jiao Liang Li Chen Yi-Feng Ge Shi-Feng Yun Bing Yao |
| author_facet | Ze He Qin Sun Yuan-Jiao Liang Li Chen Yi-Feng Ge Shi-Feng Yun Bing Yao |
| author_sort | Ze He |
| collection | DOAJ |
| description | This study was to investigate the effect of annexin A5 on testosterone secretion from primary rat Leydig cells and the underlying mechanisms. Isolated rat Leydig cells were treated with annexin A5. Testosterone production was detected by chemiluminescence assay. The protein and mRNA of Steroidogenic acute regulatory (StAR), P450scc, 3β-hydroxysteroid dehydrogenase (3β-HSD), 17β-hydroxysteroid dehydrogenase (17β-HSD), and 17α-hydroxylase were examined by Western blotting and semi-quantitative RT-PCR, respectively. Annexin A5 significantly stimulated testosterone secretion from rat Leydig cells in dose- and time-dependent manners and increased mRNA and protein expression of StAR, P450scc, 3β-HSD, and 17β-HSD but not 17α-hydroxylase. Annexin A5 knockdown by siRNA significantly decreased the level of testosterone and protein expression of P450scc, 3β-HSD, and 17β-HSD. The significant activation of ERK1/2 signaling was observed at 5, 10, and 30 min after annexin A5 treatment. After the pretreatment of Leydig cells with ERK inhibitor PD98059 (50 μmol l−1 ) for 20 min, the effects of annexin A5 on promoting testosterone secretion and increasing the expression of P450scc, 3β-HSD, and 17β-HSD were completely abrogated (P < 0.05). Thus, ERK1/2 signaling is involved in the roles of annexin A5 in mediating testosterone production and the expression of P450scc, 3β-HSD, and 17β-HSD in Leydig cells. |
| first_indexed | 2024-12-21T10:50:59Z |
| format | Article |
| id | doaj.art-880b498de50b4f63bfcaf098b20e9c8e |
| institution | Directory Open Access Journal |
| issn | 1008-682X 1745-7262 |
| language | English |
| last_indexed | 2024-12-21T10:50:59Z |
| publishDate | 2016-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Journal of Andrology |
| spelling | doaj.art-880b498de50b4f63bfcaf098b20e9c8e2022-12-21T19:06:39ZengWolters Kluwer Medknow PublicationsAsian Journal of Andrology1008-682X1745-72622016-01-0118345646110.4103/1008-682X.160260Annexin A5 regulates Leydig cell testosterone production via ERK1/2 pathwayZe HeQin SunYuan-Jiao LiangLi ChenYi-Feng GeShi-Feng YunBing YaoThis study was to investigate the effect of annexin A5 on testosterone secretion from primary rat Leydig cells and the underlying mechanisms. Isolated rat Leydig cells were treated with annexin A5. Testosterone production was detected by chemiluminescence assay. The protein and mRNA of Steroidogenic acute regulatory (StAR), P450scc, 3β-hydroxysteroid dehydrogenase (3β-HSD), 17β-hydroxysteroid dehydrogenase (17β-HSD), and 17α-hydroxylase were examined by Western blotting and semi-quantitative RT-PCR, respectively. Annexin A5 significantly stimulated testosterone secretion from rat Leydig cells in dose- and time-dependent manners and increased mRNA and protein expression of StAR, P450scc, 3β-HSD, and 17β-HSD but not 17α-hydroxylase. Annexin A5 knockdown by siRNA significantly decreased the level of testosterone and protein expression of P450scc, 3β-HSD, and 17β-HSD. The significant activation of ERK1/2 signaling was observed at 5, 10, and 30 min after annexin A5 treatment. After the pretreatment of Leydig cells with ERK inhibitor PD98059 (50 μmol l−1 ) for 20 min, the effects of annexin A5 on promoting testosterone secretion and increasing the expression of P450scc, 3β-HSD, and 17β-HSD were completely abrogated (P < 0.05). Thus, ERK1/2 signaling is involved in the roles of annexin A5 in mediating testosterone production and the expression of P450scc, 3β-HSD, and 17β-HSD in Leydig cells.http://www.ajandrology.com/article.asp?issn=1008-682X;year=2016;volume=18;issue=3;spage=456;epage=461;aulast=He17β-HSD; 3β-HSD; annexin A5; P450scc; StAR; testosterone |
| spellingShingle | Ze He Qin Sun Yuan-Jiao Liang Li Chen Yi-Feng Ge Shi-Feng Yun Bing Yao Annexin A5 regulates Leydig cell testosterone production via ERK1/2 pathway Asian Journal of Andrology 17β-HSD; 3β-HSD; annexin A5; P450scc; StAR; testosterone |
| title | Annexin A5 regulates Leydig cell testosterone production via ERK1/2 pathway |
| title_full | Annexin A5 regulates Leydig cell testosterone production via ERK1/2 pathway |
| title_fullStr | Annexin A5 regulates Leydig cell testosterone production via ERK1/2 pathway |
| title_full_unstemmed | Annexin A5 regulates Leydig cell testosterone production via ERK1/2 pathway |
| title_short | Annexin A5 regulates Leydig cell testosterone production via ERK1/2 pathway |
| title_sort | annexin a5 regulates leydig cell testosterone production via erk1 2 pathway |
| topic | 17β-HSD; 3β-HSD; annexin A5; P450scc; StAR; testosterone |
| url | http://www.ajandrology.com/article.asp?issn=1008-682X;year=2016;volume=18;issue=3;spage=456;epage=461;aulast=He |
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