Downscaling of a Three-Dimensional Alveolar In Vitro Model for High-Throughput Screening of Inhalable Respiratory Sensitizers in the Food Industry
Allergic diseases represent an increasing global health problem, among which food and respiratory allergies play an important part. Inhalation of sensitizers from food sources primes the immune system for an exacerbated response upon subsequent exposure via the ingestion route. With the increasing e...
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| Format: | Article |
| Language: | English |
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AcademicPres
2023-06-01
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| Series: | Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Food Science and Technology |
| Subjects: | |
| Online Access: | https://journals.usamvcluj.ro/index.php/fst/article/view/14593 |
| _version_ | 1797299899032666112 |
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| author | Sabina BURLA Aline CHARY Elisa MOSCHINI Charlotte B. A. STOFFELS Tommaso SERCHI Carmen SOCACIU Mihai A. SOCACIU Arno C. GUTLEB |
| author_facet | Sabina BURLA Aline CHARY Elisa MOSCHINI Charlotte B. A. STOFFELS Tommaso SERCHI Carmen SOCACIU Mihai A. SOCACIU Arno C. GUTLEB |
| author_sort | Sabina BURLA |
| collection | DOAJ |
| description | Allergic diseases represent an increasing global health problem, among which food and respiratory allergies play an important part. Inhalation of sensitizers from food sources primes the immune system for an exacerbated response upon subsequent exposure via the ingestion route. With the increasing emergence of novel food additives and proteins and growing morbidity in both food and respiratory allergies, the hazard assessment of the allergenicity of food components is of paramount importance. A three-dimensional (3D) alveolar in vitro model for respiratory sensitization prediction was downscaled from the 6- to the 24-well plate insert format, to meet the industry’s high-throughput (HTS) screening requirements. The resemblance of the cellular architecture of the downscaled model to the alveolar region of the lung was evaluated through microscopy techniques and the functionality of the in vitro model was assessed following air-liquid interface (ALI) exposure to ammonium persulfate (APS). The model represents a promising in vitro tool to screen for the respiratory sensitization potential of food components in a fast and reliable approach. |
| first_indexed | 2024-03-07T22:57:20Z |
| format | Article |
| id | doaj.art-880ce29f81d745abb3f1817de3eea359 |
| institution | Directory Open Access Journal |
| issn | 2344-2344 2344-5300 |
| language | English |
| last_indexed | 2024-03-07T22:57:20Z |
| publishDate | 2023-06-01 |
| publisher | AcademicPres |
| record_format | Article |
| series | Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Food Science and Technology |
| spelling | doaj.art-880ce29f81d745abb3f1817de3eea3592024-02-22T22:15:15ZengAcademicPresBulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Food Science and Technology2344-23442344-53002023-06-0180172310.15835/buasvmcn-fst:2023.000314593Downscaling of a Three-Dimensional Alveolar In Vitro Model for High-Throughput Screening of Inhalable Respiratory Sensitizers in the Food IndustrySabina BURLA0Aline CHARY1Elisa MOSCHINI2Charlotte B. A. STOFFELS3Tommaso SERCHI4Carmen SOCACIU5Mihai A. SOCACIU6Arno C. GUTLEB7Environmental Research and Innovation (ERIN) Department, Luxembourg Institute of Science and Technology (LIST), 41 Rue du Brill, Belvaux, L-4422Environmental Research and Innovation (ERIN) Department, Luxembourg Institute of Science and Technology (LIST), 41 Rue du Brill, Belvaux, L-4422Environmental Research and Innovation (ERIN) Department, Luxembourg Institute of Science and Technology (LIST), 41 Rue du Brill, Belvaux, L-4422Environmental Research and Innovation (ERIN) Department, Luxembourg Institute of Science and Technology (LIST), 41 Rue du Brill, Belvaux, L-4422Environmental Research and Innovation (ERIN) Department, Luxembourg Institute of Science and Technology (LIST), 41 Rue du Brill, Belvaux, L-4422Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, 3-5 Mănăştur Street, Cluj-Napoca, 400372Faculty of Medicine, University of Medicine and Pharmacy „Iuliu Hatieganu”, 19-21 Croitorilor Street, Cluj-Napoca, 400162Environmental Research and Innovation (ERIN) Department, Luxembourg Institute of Science and Technology (LIST), 41 Rue du Brill, Belvaux, L-4422Allergic diseases represent an increasing global health problem, among which food and respiratory allergies play an important part. Inhalation of sensitizers from food sources primes the immune system for an exacerbated response upon subsequent exposure via the ingestion route. With the increasing emergence of novel food additives and proteins and growing morbidity in both food and respiratory allergies, the hazard assessment of the allergenicity of food components is of paramount importance. A three-dimensional (3D) alveolar in vitro model for respiratory sensitization prediction was downscaled from the 6- to the 24-well plate insert format, to meet the industry’s high-throughput (HTS) screening requirements. The resemblance of the cellular architecture of the downscaled model to the alveolar region of the lung was evaluated through microscopy techniques and the functionality of the in vitro model was assessed following air-liquid interface (ALI) exposure to ammonium persulfate (APS). The model represents a promising in vitro tool to screen for the respiratory sensitization potential of food components in a fast and reliable approach.https://journals.usamvcluj.ro/index.php/fst/article/view/14593alveolarair-liquid interfacecell surface markerscytokinesin vitro |
| spellingShingle | Sabina BURLA Aline CHARY Elisa MOSCHINI Charlotte B. A. STOFFELS Tommaso SERCHI Carmen SOCACIU Mihai A. SOCACIU Arno C. GUTLEB Downscaling of a Three-Dimensional Alveolar In Vitro Model for High-Throughput Screening of Inhalable Respiratory Sensitizers in the Food Industry Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Food Science and Technology alveolar air-liquid interface cell surface markers cytokines in vitro |
| title | Downscaling of a Three-Dimensional Alveolar In Vitro Model for High-Throughput Screening of Inhalable Respiratory Sensitizers in the Food Industry |
| title_full | Downscaling of a Three-Dimensional Alveolar In Vitro Model for High-Throughput Screening of Inhalable Respiratory Sensitizers in the Food Industry |
| title_fullStr | Downscaling of a Three-Dimensional Alveolar In Vitro Model for High-Throughput Screening of Inhalable Respiratory Sensitizers in the Food Industry |
| title_full_unstemmed | Downscaling of a Three-Dimensional Alveolar In Vitro Model for High-Throughput Screening of Inhalable Respiratory Sensitizers in the Food Industry |
| title_short | Downscaling of a Three-Dimensional Alveolar In Vitro Model for High-Throughput Screening of Inhalable Respiratory Sensitizers in the Food Industry |
| title_sort | downscaling of a three dimensional alveolar in vitro model for high throughput screening of inhalable respiratory sensitizers in the food industry |
| topic | alveolar air-liquid interface cell surface markers cytokines in vitro |
| url | https://journals.usamvcluj.ro/index.php/fst/article/view/14593 |
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