A versatile genetic-encoded reporter for magnetic resonance imaging
It has been a long-cherished wish in biomedicine research to have an imaging tool to visualize gene expression, with good spatiotemporal resolution, in rodent and primate animals noninvasively and longitudinally. To this purpose, we here present a novel genetic encoded magnetic resonance imaging rep...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-03-01
|
| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023012616 |
| _version_ | 1797851860209500160 |
|---|---|
| author | Haiyang Yang Zhengjiang Qian Chunhua Liu Changjun Tie Aoling Cai Jie Wang Yao Xing Jun Xia Xiang Li |
| author_facet | Haiyang Yang Zhengjiang Qian Chunhua Liu Changjun Tie Aoling Cai Jie Wang Yao Xing Jun Xia Xiang Li |
| author_sort | Haiyang Yang |
| collection | DOAJ |
| description | It has been a long-cherished wish in biomedicine research to have an imaging tool to visualize gene expression, with good spatiotemporal resolution, in rodent and primate animals noninvasively and longitudinally. To this purpose, we here present a novel genetic encoded magnetic resonance imaging reporter, i.e., GEM reporter, for noninvasive visualization of cell-specific gene expression. The GEM reporter was developed through codon modification of a bacteria-originated manganese (Mn) binding protein, allowing the sequestration of endogenous Mn in local tissues. When expressed in bacteria, plant and animals, GEM reporter can robustly produce high image contrast in T1-weighted MRI without additional substrates or contrast agents. Importantly, GEM reporter can be tracked inherently by MRI in specific cells and tissues. These findings support GEM reporter as a versatile marker for deciphering gene expression spatiotemporally in living subjects. |
| first_indexed | 2024-04-09T19:24:38Z |
| format | Article |
| id | doaj.art-880f0b026aaa47afa011b65475fb9bdf |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-09T19:24:38Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-880f0b026aaa47afa011b65475fb9bdf2023-04-05T08:20:32ZengElsevierHeliyon2405-84402023-03-0193e14054A versatile genetic-encoded reporter for magnetic resonance imagingHaiyang Yang0Zhengjiang Qian1Chunhua Liu2Changjun Tie3Aoling Cai4Jie Wang5Yao Xing6Jun Xia7Xiang Li8Guangdong Provincial Key Laboratory of Brain Connectome and Behavior, Shenzhen Key Laboratory of Viral Vectors for Biomedicine, Brain Cognition and Brain Disease Institute (BCBDI), Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China; University of Chinese Academy of Sciences, Beijing, 100049, ChinaGuangdong Provincial Key Laboratory of Brain Connectome and Behavior, Shenzhen Key Laboratory of Viral Vectors for Biomedicine, Brain Cognition and Brain Disease Institute (BCBDI), Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, ChinaGuangdong Provincial Key Laboratory of Brain Connectome and Behavior, Shenzhen Key Laboratory of Viral Vectors for Biomedicine, Brain Cognition and Brain Disease Institute (BCBDI), Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, ChinaInstitute of Computing Technology, Chinese Academy of Sciences, Beijing, China; Peng Cheng Laboratory, Shenzhen, ChinaUniversity of Chinese Academy of Sciences, Beijing, 100049, China; Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences-Wuhan National Laboratory for Optoelectronics, 430071, Wuhan, Hubei Province, ChinaKey Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences-Wuhan National Laboratory for Optoelectronics, 430071, Wuhan, Hubei Province, ChinaShanghai United Imaging Healthcare Co., Ltd., ChinaDepartment of Radiology, The First Affiliated Hospital of Shenzhen University, Health Science Center, Shenzhen Second People’s Hospital, 3002 SunGang Road West, Shenzhen 518035, ChinaGuangdong Provincial Key Laboratory of Brain Connectome and Behavior, Shenzhen Key Laboratory of Viral Vectors for Biomedicine, Brain Cognition and Brain Disease Institute (BCBDI), Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China; Corresponding author.It has been a long-cherished wish in biomedicine research to have an imaging tool to visualize gene expression, with good spatiotemporal resolution, in rodent and primate animals noninvasively and longitudinally. To this purpose, we here present a novel genetic encoded magnetic resonance imaging reporter, i.e., GEM reporter, for noninvasive visualization of cell-specific gene expression. The GEM reporter was developed through codon modification of a bacteria-originated manganese (Mn) binding protein, allowing the sequestration of endogenous Mn in local tissues. When expressed in bacteria, plant and animals, GEM reporter can robustly produce high image contrast in T1-weighted MRI without additional substrates or contrast agents. Importantly, GEM reporter can be tracked inherently by MRI in specific cells and tissues. These findings support GEM reporter as a versatile marker for deciphering gene expression spatiotemporally in living subjects.http://www.sciencedirect.com/science/article/pii/S2405844023012616MRIMolecular imagingContrast agent |
| spellingShingle | Haiyang Yang Zhengjiang Qian Chunhua Liu Changjun Tie Aoling Cai Jie Wang Yao Xing Jun Xia Xiang Li A versatile genetic-encoded reporter for magnetic resonance imaging Heliyon MRI Molecular imaging Contrast agent |
| title | A versatile genetic-encoded reporter for magnetic resonance imaging |
| title_full | A versatile genetic-encoded reporter for magnetic resonance imaging |
| title_fullStr | A versatile genetic-encoded reporter for magnetic resonance imaging |
| title_full_unstemmed | A versatile genetic-encoded reporter for magnetic resonance imaging |
| title_short | A versatile genetic-encoded reporter for magnetic resonance imaging |
| title_sort | versatile genetic encoded reporter for magnetic resonance imaging |
| topic | MRI Molecular imaging Contrast agent |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023012616 |
| work_keys_str_mv | AT haiyangyang aversatilegeneticencodedreporterformagneticresonanceimaging AT zhengjiangqian aversatilegeneticencodedreporterformagneticresonanceimaging AT chunhualiu aversatilegeneticencodedreporterformagneticresonanceimaging AT changjuntie aversatilegeneticencodedreporterformagneticresonanceimaging AT aolingcai aversatilegeneticencodedreporterformagneticresonanceimaging AT jiewang aversatilegeneticencodedreporterformagneticresonanceimaging AT yaoxing aversatilegeneticencodedreporterformagneticresonanceimaging AT junxia aversatilegeneticencodedreporterformagneticresonanceimaging AT xiangli aversatilegeneticencodedreporterformagneticresonanceimaging AT haiyangyang versatilegeneticencodedreporterformagneticresonanceimaging AT zhengjiangqian versatilegeneticencodedreporterformagneticresonanceimaging AT chunhualiu versatilegeneticencodedreporterformagneticresonanceimaging AT changjuntie versatilegeneticencodedreporterformagneticresonanceimaging AT aolingcai versatilegeneticencodedreporterformagneticresonanceimaging AT jiewang versatilegeneticencodedreporterformagneticresonanceimaging AT yaoxing versatilegeneticencodedreporterformagneticresonanceimaging AT junxia versatilegeneticencodedreporterformagneticresonanceimaging AT xiangli versatilegeneticencodedreporterformagneticresonanceimaging |