Genome-Wide Identification and Expression Analysis of Fatty Acid Desaturase (<i>FAD</i>) Genes in <i>Camelina sativa</i> (L.) Crantz
<i>Camelina sativa</i> (L.) Crantz is an indispensable oilseed crop, and its seeds contain many unsaturated fatty acids. FAD (fatty acid desaturase) regulates the synthesis of unsaturated fatty acids. In this research, we performed <i>CsFAD</i> gene family analysis and identi...
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MDPI AG
2022-11-01
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| Online Access: | https://www.mdpi.com/1422-0067/23/23/14550 |
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| author | Daqian Sun Weizhu Quan Di Wang Jingyan Cui Tianyi Wang Mei Lin Yijin Wang Nan Wang Yuanyuan Dong Xiaowei Li Weican Liu Fawei Wang |
| author_facet | Daqian Sun Weizhu Quan Di Wang Jingyan Cui Tianyi Wang Mei Lin Yijin Wang Nan Wang Yuanyuan Dong Xiaowei Li Weican Liu Fawei Wang |
| author_sort | Daqian Sun |
| collection | DOAJ |
| description | <i>Camelina sativa</i> (L.) Crantz is an indispensable oilseed crop, and its seeds contain many unsaturated fatty acids. FAD (fatty acid desaturase) regulates the synthesis of unsaturated fatty acids. In this research, we performed <i>CsFAD</i> gene family analysis and identified 24 <i>CsFAD</i> genes in Camelina, which were unevenly distributed on 14 of the 19 total chromosomes. Phylogenetic analysis showed that <i>CsFAD</i> includes four subfamilies, supported by the conserved structures and motifs of <i>CsFAD</i> genes. In addition, we investigated the expression patterns of the <i>FAD</i> family in the different tissues of Camelina. We found that <i>CsFAD</i> family genes were all expressed in the stem, and <i>CsFAD2-2</i> was highly expressed in the early stage of seed development. Moreover, during low temperature (4 °C) stress, we identified that the expression level of <i>CsFAD2-2</i> significantly changed. By observing the transient expression of CsFAD2-2 in Arabidopsis protoplasts, we found that CsFAD2-2 was located on the nucleus. Through the detection and analysis of fatty acids, we prove that CsFAD2-2 is involved in the synthesis of linolenic acid (C18:3). In conclusion, we identified <i>CsFAD2-2</i> through the phylogenetic analysis of the <i>CsFAD</i> gene family and further determined the fatty acid content to find that <i>CsFAD2-2</i> is involved in fatty acid synthesis in Camelina. |
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| last_indexed | 2024-03-09T17:47:35Z |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-88109d7090d542cc9ff3740d1c9b13e82023-11-24T11:03:51ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-11-0123231455010.3390/ijms232314550Genome-Wide Identification and Expression Analysis of Fatty Acid Desaturase (<i>FAD</i>) Genes in <i>Camelina sativa</i> (L.) CrantzDaqian Sun0Weizhu Quan1Di Wang2Jingyan Cui3Tianyi Wang4Mei Lin5Yijin Wang6Nan Wang7Yuanyuan Dong8Xiaowei Li9Weican Liu10Fawei Wang11College of Life Sciences, Jilin Agricultural University, Changchun 130118, ChinaCollege of Life Sciences, Jilin Agricultural University, Changchun 130118, ChinaCollege of Life Sciences, Jilin Agricultural University, Changchun 130118, ChinaCollege of Life Sciences, Jilin Agricultural University, Changchun 130118, ChinaCollege of Life Sciences, Jilin Agricultural University, Changchun 130118, ChinaCollege of Life Sciences, Jilin Agricultural University, Changchun 130118, ChinaCollege of Life Sciences, Jilin Agricultural University, Changchun 130118, ChinaCollege of Life Sciences, Jilin Agricultural University, Changchun 130118, ChinaCollege of Life Sciences, Jilin Agricultural University, Changchun 130118, ChinaCollege of Life Sciences, Jilin Agricultural University, Changchun 130118, ChinaCollege of Life Sciences, Jilin Agricultural University, Changchun 130118, ChinaCollege of Life Sciences, Jilin Agricultural University, Changchun 130118, China<i>Camelina sativa</i> (L.) Crantz is an indispensable oilseed crop, and its seeds contain many unsaturated fatty acids. FAD (fatty acid desaturase) regulates the synthesis of unsaturated fatty acids. In this research, we performed <i>CsFAD</i> gene family analysis and identified 24 <i>CsFAD</i> genes in Camelina, which were unevenly distributed on 14 of the 19 total chromosomes. Phylogenetic analysis showed that <i>CsFAD</i> includes four subfamilies, supported by the conserved structures and motifs of <i>CsFAD</i> genes. In addition, we investigated the expression patterns of the <i>FAD</i> family in the different tissues of Camelina. We found that <i>CsFAD</i> family genes were all expressed in the stem, and <i>CsFAD2-2</i> was highly expressed in the early stage of seed development. Moreover, during low temperature (4 °C) stress, we identified that the expression level of <i>CsFAD2-2</i> significantly changed. By observing the transient expression of CsFAD2-2 in Arabidopsis protoplasts, we found that CsFAD2-2 was located on the nucleus. Through the detection and analysis of fatty acids, we prove that CsFAD2-2 is involved in the synthesis of linolenic acid (C18:3). In conclusion, we identified <i>CsFAD2-2</i> through the phylogenetic analysis of the <i>CsFAD</i> gene family and further determined the fatty acid content to find that <i>CsFAD2-2</i> is involved in fatty acid synthesis in Camelina.https://www.mdpi.com/1422-0067/23/23/14550<i>Camelina sativa</i> (L.) Crantzfatty acid desaturase (FAD2-2)gene familylow temperature (4 °C)linolenic acid (C18:3) |
| spellingShingle | Daqian Sun Weizhu Quan Di Wang Jingyan Cui Tianyi Wang Mei Lin Yijin Wang Nan Wang Yuanyuan Dong Xiaowei Li Weican Liu Fawei Wang Genome-Wide Identification and Expression Analysis of Fatty Acid Desaturase (<i>FAD</i>) Genes in <i>Camelina sativa</i> (L.) Crantz International Journal of Molecular Sciences <i>Camelina sativa</i> (L.) Crantz fatty acid desaturase (FAD2-2) gene family low temperature (4 °C) linolenic acid (C18:3) |
| title | Genome-Wide Identification and Expression Analysis of Fatty Acid Desaturase (<i>FAD</i>) Genes in <i>Camelina sativa</i> (L.) Crantz |
| title_full | Genome-Wide Identification and Expression Analysis of Fatty Acid Desaturase (<i>FAD</i>) Genes in <i>Camelina sativa</i> (L.) Crantz |
| title_fullStr | Genome-Wide Identification and Expression Analysis of Fatty Acid Desaturase (<i>FAD</i>) Genes in <i>Camelina sativa</i> (L.) Crantz |
| title_full_unstemmed | Genome-Wide Identification and Expression Analysis of Fatty Acid Desaturase (<i>FAD</i>) Genes in <i>Camelina sativa</i> (L.) Crantz |
| title_short | Genome-Wide Identification and Expression Analysis of Fatty Acid Desaturase (<i>FAD</i>) Genes in <i>Camelina sativa</i> (L.) Crantz |
| title_sort | genome wide identification and expression analysis of fatty acid desaturase i fad i genes in i camelina sativa i l crantz |
| topic | <i>Camelina sativa</i> (L.) Crantz fatty acid desaturase (FAD2-2) gene family low temperature (4 °C) linolenic acid (C18:3) |
| url | https://www.mdpi.com/1422-0067/23/23/14550 |
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