Antibody Microarray Expression Profiling Of Maize Roots Treated With Cadmium And Nickel
Here, we report the effectiveness of antibody microarray expression profiling (AbMEP) procedure to determine similarities and differences between two maize cultivars (Premia and Blitz) exposed to various concentration of cadmium (Cd), nickel (Ni), as well as simultaneous exposure to both metals (Cd...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Sciendo
2015-06-01
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| Series: | Agriculture |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/agri-2015-0009 |
| _version_ | 1819350203709259776 |
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| author | Repka Vladimír Fiala Roderik Čiamporová Milada Martinka Michal Pavlovkin Ján |
| author_facet | Repka Vladimír Fiala Roderik Čiamporová Milada Martinka Michal Pavlovkin Ján |
| author_sort | Repka Vladimír |
| collection | DOAJ |
| description | Here, we report the effectiveness of antibody microarray expression profiling (AbMEP) procedure to determine similarities and differences between two maize cultivars (Premia and Blitz) exposed to various concentration of cadmium (Cd), nickel (Ni), as well as simultaneous exposure to both metals (Cd + Ni) for 0, 12 and 24 h. After protein extraction from control (untreated) and heavy metals (HM)-treated root pairs and their fluorescent labelling, the protein extracts were used for the AbMEP procedure and western blot analysis. The results from the microarray were analysed using an internally normalised ratio. Using this highly parallel AbMEP-approach, the timing, dynamics and regulation of the expression of 101 specific genes in untreated and HM-treated roots of maize cultivars were determined. The microarray results revealed 23, 15 and 10 up-regulated/down-regulated proteins in Cd, Ni and Cd + Ni treated cv. Premia roots, respectively. In contrast, the microarray results revealed that 15, 11 and 7 proteins were up-regulated/down-regulated in Cd, Ni and Cd + Ni treated cv. Blitz roots, respectively. The data obtained from the AbMEP array experiment were validated by western blot analyses with more than 89% (the Pearson’s correlation coeficient Rr = 0.78) correlation between the two methods. |
| first_indexed | 2024-12-24T19:12:41Z |
| format | Article |
| id | doaj.art-8811bfd2c6854cd0b788deb7ab266009 |
| institution | Directory Open Access Journal |
| issn | 1338-4376 |
| language | English |
| last_indexed | 2024-12-24T19:12:41Z |
| publishDate | 2015-06-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Agriculture |
| spelling | doaj.art-8811bfd2c6854cd0b788deb7ab2660092022-12-21T16:42:58ZengSciendoAgriculture1338-43762015-06-01612414910.1515/agri-2015-0009agri-2015-0009Antibody Microarray Expression Profiling Of Maize Roots Treated With Cadmium And NickelRepka Vladimír0Fiala Roderik1Čiamporová Milada2Martinka Michal3Pavlovkin Ján4Institute of Botany, Slovak Academy of Sciences, BratislavaInstitute of Botany, Slovak Academy of Sciences, BratislavaInstitute of Botany, Slovak Academy of Sciences, BratislavaInstitute of Botany, Slovak Academy of Sciences, BratislavaInstitute of Botany, Slovak Academy of Sciences, BratislavaHere, we report the effectiveness of antibody microarray expression profiling (AbMEP) procedure to determine similarities and differences between two maize cultivars (Premia and Blitz) exposed to various concentration of cadmium (Cd), nickel (Ni), as well as simultaneous exposure to both metals (Cd + Ni) for 0, 12 and 24 h. After protein extraction from control (untreated) and heavy metals (HM)-treated root pairs and their fluorescent labelling, the protein extracts were used for the AbMEP procedure and western blot analysis. The results from the microarray were analysed using an internally normalised ratio. Using this highly parallel AbMEP-approach, the timing, dynamics and regulation of the expression of 101 specific genes in untreated and HM-treated roots of maize cultivars were determined. The microarray results revealed 23, 15 and 10 up-regulated/down-regulated proteins in Cd, Ni and Cd + Ni treated cv. Premia roots, respectively. In contrast, the microarray results revealed that 15, 11 and 7 proteins were up-regulated/down-regulated in Cd, Ni and Cd + Ni treated cv. Blitz roots, respectively. The data obtained from the AbMEP array experiment were validated by western blot analyses with more than 89% (the Pearson’s correlation coeficient Rr = 0.78) correlation between the two methods.https://doi.org/10.1515/agri-2015-0009maize rootsheavy metalsproteomicsprofilingmicroarray |
| spellingShingle | Repka Vladimír Fiala Roderik Čiamporová Milada Martinka Michal Pavlovkin Ján Antibody Microarray Expression Profiling Of Maize Roots Treated With Cadmium And Nickel Agriculture maize roots heavy metals proteomics profiling microarray |
| title | Antibody Microarray Expression Profiling Of Maize Roots Treated With Cadmium And Nickel |
| title_full | Antibody Microarray Expression Profiling Of Maize Roots Treated With Cadmium And Nickel |
| title_fullStr | Antibody Microarray Expression Profiling Of Maize Roots Treated With Cadmium And Nickel |
| title_full_unstemmed | Antibody Microarray Expression Profiling Of Maize Roots Treated With Cadmium And Nickel |
| title_short | Antibody Microarray Expression Profiling Of Maize Roots Treated With Cadmium And Nickel |
| title_sort | antibody microarray expression profiling of maize roots treated with cadmium and nickel |
| topic | maize roots heavy metals proteomics profiling microarray |
| url | https://doi.org/10.1515/agri-2015-0009 |
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