Effects of Bilberry Extract on Hepatic Cholesterol Metabolism in HepG2 Cells
Bilberry (<i>Vaccinium myrtillus</i> L.), rich in polyphenols, has been claimed to have lipid-lowering effects, but its underlying mechanisms remain unclear. The effects of bilberry extract (BE) with antioxidant properties on hepatic lipid metabolism were investigated by measuring the ge...
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| Format: | Article |
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MDPI AG
2022-12-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/13/1/516 |
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| author | Jimin Hong Minkyung Bae Bohkyung Kim |
| author_facet | Jimin Hong Minkyung Bae Bohkyung Kim |
| author_sort | Jimin Hong |
| collection | DOAJ |
| description | Bilberry (<i>Vaccinium myrtillus</i> L.), rich in polyphenols, has been claimed to have lipid-lowering effects, but its underlying mechanisms remain unclear. The effects of bilberry extract (BE) with antioxidant properties on hepatic lipid metabolism were investigated by measuring the genes for cholesterol biosynthesis and flux in HepG2 cells. The mRNA and protein levels of genes involved in cholesterol biosynthesis such as sterol regulatory element-binding protein 2 and 3-hydroxy-3-methylglutaryl coenzyme A reductase were decreased in BE-treated cells. BE posttranscriptionally upregulated low-density lipoprotein receptor in HepG2 cells. There was a marked reduction in genes for very low-density lipoprotein assembly by BE treatment. Furthermore, the expression of canalicular transporter for cholesterol and bile acids, such as ABCG8 and ABCB11, was significantly elevated by BE treatment. Downregulation of lipogenic genes and upregulation of fatty acid oxidation-related genes were observed in BE-treated HepG2 cells. The expressions of sirtuins were altered by BE treatment. These results support that the effects of BE on hepatic cholesterol metabolism may be attributed to the regulation of genes for hepatic cholesterol biosynthesis, transport and efflux. |
| first_indexed | 2024-03-11T10:07:57Z |
| format | Article |
| id | doaj.art-8811febc78504bcdaede8f86288ac679 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-11T10:07:57Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-8811febc78504bcdaede8f86288ac6792023-11-16T14:58:03ZengMDPI AGApplied Sciences2076-34172022-12-0113151610.3390/app13010516Effects of Bilberry Extract on Hepatic Cholesterol Metabolism in HepG2 CellsJimin Hong0Minkyung Bae1Bohkyung Kim2Department of Food Science and Nutrition, Pusan National University, Busan 46241, Republic of KoreaDepartment of Food and Nutrition, Changwon National University, Changwon 51140, Republic of KoreaDepartment of Food Science and Nutrition, Pusan National University, Busan 46241, Republic of KoreaBilberry (<i>Vaccinium myrtillus</i> L.), rich in polyphenols, has been claimed to have lipid-lowering effects, but its underlying mechanisms remain unclear. The effects of bilberry extract (BE) with antioxidant properties on hepatic lipid metabolism were investigated by measuring the genes for cholesterol biosynthesis and flux in HepG2 cells. The mRNA and protein levels of genes involved in cholesterol biosynthesis such as sterol regulatory element-binding protein 2 and 3-hydroxy-3-methylglutaryl coenzyme A reductase were decreased in BE-treated cells. BE posttranscriptionally upregulated low-density lipoprotein receptor in HepG2 cells. There was a marked reduction in genes for very low-density lipoprotein assembly by BE treatment. Furthermore, the expression of canalicular transporter for cholesterol and bile acids, such as ABCG8 and ABCB11, was significantly elevated by BE treatment. Downregulation of lipogenic genes and upregulation of fatty acid oxidation-related genes were observed in BE-treated HepG2 cells. The expressions of sirtuins were altered by BE treatment. These results support that the effects of BE on hepatic cholesterol metabolism may be attributed to the regulation of genes for hepatic cholesterol biosynthesis, transport and efflux.https://www.mdpi.com/2076-3417/13/1/516bilberry extractcholesterolhypercholesterolemiacardiovascular disease |
| spellingShingle | Jimin Hong Minkyung Bae Bohkyung Kim Effects of Bilberry Extract on Hepatic Cholesterol Metabolism in HepG2 Cells Applied Sciences bilberry extract cholesterol hypercholesterolemia cardiovascular disease |
| title | Effects of Bilberry Extract on Hepatic Cholesterol Metabolism in HepG2 Cells |
| title_full | Effects of Bilberry Extract on Hepatic Cholesterol Metabolism in HepG2 Cells |
| title_fullStr | Effects of Bilberry Extract on Hepatic Cholesterol Metabolism in HepG2 Cells |
| title_full_unstemmed | Effects of Bilberry Extract on Hepatic Cholesterol Metabolism in HepG2 Cells |
| title_short | Effects of Bilberry Extract on Hepatic Cholesterol Metabolism in HepG2 Cells |
| title_sort | effects of bilberry extract on hepatic cholesterol metabolism in hepg2 cells |
| topic | bilberry extract cholesterol hypercholesterolemia cardiovascular disease |
| url | https://www.mdpi.com/2076-3417/13/1/516 |
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