Asymmetric random walks reveal that the chemotaxis network modulates flagellar rotational bias in Helicobacter pylori
The canonical chemotaxis network modulates the bias for a particular direction of rotation in the bacterial flagellar motor to help the cell migrate toward favorable chemical environments. How the chemotaxis network in Helicobacter pylori modulates flagellar functions is unknown, which limits our un...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2021-01-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/63936 |
| _version_ | 1828167944992980992 |
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| author | Jyot D Antani Anita X Sumali Tanmay P Lele Pushkar P Lele |
| author_facet | Jyot D Antani Anita X Sumali Tanmay P Lele Pushkar P Lele |
| author_sort | Jyot D Antani |
| collection | DOAJ |
| description | The canonical chemotaxis network modulates the bias for a particular direction of rotation in the bacterial flagellar motor to help the cell migrate toward favorable chemical environments. How the chemotaxis network in Helicobacter pylori modulates flagellar functions is unknown, which limits our understanding of chemotaxis in this species. Here, we determined that H. pylori swim faster (slower) whenever their flagella rotate counterclockwise (clockwise) by analyzing their hydrodynamic interactions with bounding surfaces. This asymmetry in swimming helped quantify the rotational bias. Upon exposure to a chemo-attractant, the bias decreased and the cells tended to swim exclusively in the faster mode. In the absence of a key chemotaxis protein, CheY, the bias was zero. The relationship between the reversal frequency and the rotational bias was unimodal. Thus, H. pylori’s chemotaxis network appears to modulate the probability of clockwise rotation in otherwise counterclockwise-rotating flagella, similar to the canonical network. |
| first_indexed | 2024-04-12T02:25:46Z |
| format | Article |
| id | doaj.art-8813e60ee4424148815b0b713d7d6283 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T02:25:46Z |
| publishDate | 2021-01-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-8813e60ee4424148815b0b713d7d62832022-12-22T03:52:00ZengeLife Sciences Publications LtdeLife2050-084X2021-01-011010.7554/eLife.63936Asymmetric random walks reveal that the chemotaxis network modulates flagellar rotational bias in Helicobacter pyloriJyot D Antani0https://orcid.org/0000-0002-7402-983XAnita X Sumali1Tanmay P Lele2Pushkar P Lele3https://orcid.org/0000-0002-2894-3487Artie McFerrin Department of Chemical Engineering, Texas A&M University, College Station, TX 77843, United StatesArtie McFerrin Department of Chemical Engineering, Texas A&M University, College Station, TX 77843, United StatesDepartment of Biomedical Engineering, Texas A&M University, College Station, TX 77840, College Station, TX 77840, United States; Department of Translational Medical Sciences, Texas A&M University, Houston, TX 77030, United StatesArtie McFerrin Department of Chemical Engineering, Texas A&M University, College Station, TX 77843, United StatesThe canonical chemotaxis network modulates the bias for a particular direction of rotation in the bacterial flagellar motor to help the cell migrate toward favorable chemical environments. How the chemotaxis network in Helicobacter pylori modulates flagellar functions is unknown, which limits our understanding of chemotaxis in this species. Here, we determined that H. pylori swim faster (slower) whenever their flagella rotate counterclockwise (clockwise) by analyzing their hydrodynamic interactions with bounding surfaces. This asymmetry in swimming helped quantify the rotational bias. Upon exposure to a chemo-attractant, the bias decreased and the cells tended to swim exclusively in the faster mode. In the absence of a key chemotaxis protein, CheY, the bias was zero. The relationship between the reversal frequency and the rotational bias was unimodal. Thus, H. pylori’s chemotaxis network appears to modulate the probability of clockwise rotation in otherwise counterclockwise-rotating flagella, similar to the canonical network.https://elifesciences.org/articles/63936Helicobacter pylorichemotaxisflagellaswitchingreversals |
| spellingShingle | Jyot D Antani Anita X Sumali Tanmay P Lele Pushkar P Lele Asymmetric random walks reveal that the chemotaxis network modulates flagellar rotational bias in Helicobacter pylori eLife Helicobacter pylori chemotaxis flagella switching reversals |
| title | Asymmetric random walks reveal that the chemotaxis network modulates flagellar rotational bias in Helicobacter pylori |
| title_full | Asymmetric random walks reveal that the chemotaxis network modulates flagellar rotational bias in Helicobacter pylori |
| title_fullStr | Asymmetric random walks reveal that the chemotaxis network modulates flagellar rotational bias in Helicobacter pylori |
| title_full_unstemmed | Asymmetric random walks reveal that the chemotaxis network modulates flagellar rotational bias in Helicobacter pylori |
| title_short | Asymmetric random walks reveal that the chemotaxis network modulates flagellar rotational bias in Helicobacter pylori |
| title_sort | asymmetric random walks reveal that the chemotaxis network modulates flagellar rotational bias in helicobacter pylori |
| topic | Helicobacter pylori chemotaxis flagella switching reversals |
| url | https://elifesciences.org/articles/63936 |
| work_keys_str_mv | AT jyotdantani asymmetricrandomwalksrevealthatthechemotaxisnetworkmodulatesflagellarrotationalbiasinhelicobacterpylori AT anitaxsumali asymmetricrandomwalksrevealthatthechemotaxisnetworkmodulatesflagellarrotationalbiasinhelicobacterpylori AT tanmayplele asymmetricrandomwalksrevealthatthechemotaxisnetworkmodulatesflagellarrotationalbiasinhelicobacterpylori AT pushkarplele asymmetricrandomwalksrevealthatthechemotaxisnetworkmodulatesflagellarrotationalbiasinhelicobacterpylori |