Catalytic RNA Oligomers Formed by Co-Oligomerization of a Pair of Bimolecular RNase P Ribozymes
Naturally occurring ribozymes with a modular architecture are promising platforms for construction of RNA nanostructures because modular redesign enables their oligomerization. The resulting RNA nanostructures can exhibit the catalytic function of the parent ribozyme in an assembly dependent manner....
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/23/8298 |
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| author | Mst. Ayesha Siddika Takahiro Yamada Risako Aoyama Kumi Hidaka Hiroshi Sugiyama Masayuki Endo Shigeyoshi Matsumura Yoshiya Ikawa |
| author_facet | Mst. Ayesha Siddika Takahiro Yamada Risako Aoyama Kumi Hidaka Hiroshi Sugiyama Masayuki Endo Shigeyoshi Matsumura Yoshiya Ikawa |
| author_sort | Mst. Ayesha Siddika |
| collection | DOAJ |
| description | Naturally occurring ribozymes with a modular architecture are promising platforms for construction of RNA nanostructures because modular redesign enables their oligomerization. The resulting RNA nanostructures can exhibit the catalytic function of the parent ribozyme in an assembly dependent manner. In this study, we designed and constructed open-form oligomers of a bimolecular form of an RNase P ribozyme. The ribozyme oligomers were analyzed biochemically and by atomic force microscopy (AFM). |
| first_indexed | 2024-03-09T17:39:46Z |
| format | Article |
| id | doaj.art-88148bfad5224568ad99fe843a67cccd |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T17:39:46Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-88148bfad5224568ad99fe843a67cccd2023-11-24T11:39:55ZengMDPI AGMolecules1420-30492022-11-012723829810.3390/molecules27238298Catalytic RNA Oligomers Formed by Co-Oligomerization of a Pair of Bimolecular RNase P RibozymesMst. Ayesha Siddika0Takahiro Yamada1Risako Aoyama2Kumi Hidaka3Hiroshi Sugiyama4Masayuki Endo5Shigeyoshi Matsumura6Yoshiya Ikawa7Graduate School of Innovative Life Science, University of Toyama, Gofuku 3190, Toyama 930-8555, JapanDepartment of Chemistry, Graduate School of Science and Engineering, University of Toyama, Gofuku 3190, Toyama 930-8555, JapanDepartment of Chemistry, Graduate School of Science and Engineering, University of Toyama, Gofuku 3190, Toyama 930-8555, JapanInstitute for Integrated Cell-Material Sciences, Kyoto University, Kyoto 606-8501, JapanInstitute for Integrated Cell-Material Sciences, Kyoto University, Kyoto 606-8501, JapanInstitute for Integrated Cell-Material Sciences, Kyoto University, Kyoto 606-8501, JapanGraduate School of Innovative Life Science, University of Toyama, Gofuku 3190, Toyama 930-8555, JapanGraduate School of Innovative Life Science, University of Toyama, Gofuku 3190, Toyama 930-8555, JapanNaturally occurring ribozymes with a modular architecture are promising platforms for construction of RNA nanostructures because modular redesign enables their oligomerization. The resulting RNA nanostructures can exhibit the catalytic function of the parent ribozyme in an assembly dependent manner. In this study, we designed and constructed open-form oligomers of a bimolecular form of an RNase P ribozyme. The ribozyme oligomers were analyzed biochemically and by atomic force microscopy (AFM).https://www.mdpi.com/1420-3049/27/23/8298ribonuclease PribozymeRNA motifRNA nanostructurepre-tRNA processing |
| spellingShingle | Mst. Ayesha Siddika Takahiro Yamada Risako Aoyama Kumi Hidaka Hiroshi Sugiyama Masayuki Endo Shigeyoshi Matsumura Yoshiya Ikawa Catalytic RNA Oligomers Formed by Co-Oligomerization of a Pair of Bimolecular RNase P Ribozymes Molecules ribonuclease P ribozyme RNA motif RNA nanostructure pre-tRNA processing |
| title | Catalytic RNA Oligomers Formed by Co-Oligomerization of a Pair of Bimolecular RNase P Ribozymes |
| title_full | Catalytic RNA Oligomers Formed by Co-Oligomerization of a Pair of Bimolecular RNase P Ribozymes |
| title_fullStr | Catalytic RNA Oligomers Formed by Co-Oligomerization of a Pair of Bimolecular RNase P Ribozymes |
| title_full_unstemmed | Catalytic RNA Oligomers Formed by Co-Oligomerization of a Pair of Bimolecular RNase P Ribozymes |
| title_short | Catalytic RNA Oligomers Formed by Co-Oligomerization of a Pair of Bimolecular RNase P Ribozymes |
| title_sort | catalytic rna oligomers formed by co oligomerization of a pair of bimolecular rnase p ribozymes |
| topic | ribonuclease P ribozyme RNA motif RNA nanostructure pre-tRNA processing |
| url | https://www.mdpi.com/1420-3049/27/23/8298 |
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