Global genome analysis of the downstream binding targets of testis determining factor SRY and SOX9.
A major event in mammalian male sex determination is the induction of the testis determining factor Sry and its downstream gene Sox9. The current study provides one of the first genome wide analyses of the downstream gene binding targets for SRY and SOX9 to help elucidate the molecular control of Se...
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3440412?pdf=render |
| _version_ | 1819082363799339008 |
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| author | Ramji K Bhandari Md M Haque Michael K Skinner |
| author_facet | Ramji K Bhandari Md M Haque Michael K Skinner |
| author_sort | Ramji K Bhandari |
| collection | DOAJ |
| description | A major event in mammalian male sex determination is the induction of the testis determining factor Sry and its downstream gene Sox9. The current study provides one of the first genome wide analyses of the downstream gene binding targets for SRY and SOX9 to help elucidate the molecular control of Sertoli cell differentiation and testis development. A modified ChIP-Chip analysis using a comparative hybridization was used to identify 71 direct downstream binding targets for SRY and 109 binding targets for SOX9. Interestingly, only 5 gene targets overlapped between SRY and SOX9. In addition to the direct response element binding gene targets, a large number of atypical binding gene targets were identified for both SRY and SOX9. Bioinformatic analysis of the downstream binding targets identified gene networks and cellular pathways potentially involved in the induction of Sertoli cell differentiation and testis development. The specific DNA sequence binding site motifs for both SRY and SOX9 were identified. Observations provide insights into the molecular control of male gonadal sex determination. |
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| format | Article |
| id | doaj.art-8814ab82b5a54ff69a3da497f7c8eaa5 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T20:15:29Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-8814ab82b5a54ff69a3da497f7c8eaa52022-12-21T18:51:38ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0179e4338010.1371/journal.pone.0043380Global genome analysis of the downstream binding targets of testis determining factor SRY and SOX9.Ramji K BhandariMd M HaqueMichael K SkinnerA major event in mammalian male sex determination is the induction of the testis determining factor Sry and its downstream gene Sox9. The current study provides one of the first genome wide analyses of the downstream gene binding targets for SRY and SOX9 to help elucidate the molecular control of Sertoli cell differentiation and testis development. A modified ChIP-Chip analysis using a comparative hybridization was used to identify 71 direct downstream binding targets for SRY and 109 binding targets for SOX9. Interestingly, only 5 gene targets overlapped between SRY and SOX9. In addition to the direct response element binding gene targets, a large number of atypical binding gene targets were identified for both SRY and SOX9. Bioinformatic analysis of the downstream binding targets identified gene networks and cellular pathways potentially involved in the induction of Sertoli cell differentiation and testis development. The specific DNA sequence binding site motifs for both SRY and SOX9 were identified. Observations provide insights into the molecular control of male gonadal sex determination.http://europepmc.org/articles/PMC3440412?pdf=render |
| spellingShingle | Ramji K Bhandari Md M Haque Michael K Skinner Global genome analysis of the downstream binding targets of testis determining factor SRY and SOX9. PLoS ONE |
| title | Global genome analysis of the downstream binding targets of testis determining factor SRY and SOX9. |
| title_full | Global genome analysis of the downstream binding targets of testis determining factor SRY and SOX9. |
| title_fullStr | Global genome analysis of the downstream binding targets of testis determining factor SRY and SOX9. |
| title_full_unstemmed | Global genome analysis of the downstream binding targets of testis determining factor SRY and SOX9. |
| title_short | Global genome analysis of the downstream binding targets of testis determining factor SRY and SOX9. |
| title_sort | global genome analysis of the downstream binding targets of testis determining factor sry and sox9 |
| url | http://europepmc.org/articles/PMC3440412?pdf=render |
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