| Summary: | Based on the five phases of land use data from Xining corresponding to 1980, 1990, 2000, 2010, and 2020, we used the equivalent factor method to analyze the values of spatial-temporal variation characteristics of land use and ecosystem services in Xining. The results showed that (1) farmland and developed areas were the most active types of land, and the continuous occupation of farmland and developed areas led to the formation of a kind of “cross shape” in the spatial pattern of conversion of land use types along the Huangshui River, Beichuan River, and Nanchuan River from south to north and from northwest to southeast, respectively, with the central urban area serving as a core of the pattern; (2) the transformation between different land use types mainly occurred in the land-slope range between 0–15° and altitudes between 2000–2750 m; and (3) the ESV of Xining increased by RMB 2165.26 × 10<sup>6</sup> in the past 40 years. The period from 2000 to 2020 was the main growth period of the ecosystem service value of Xining. Urbanization had a great impact on the variation in land use types and the evolution of ecosystem service values. In the middle and late stages of urbanization, different types of land use changed significantly within each county and district. The ecosystem service values of the central districts were low, with those of the marginal districts and counties being higher, forming a “core-periphery” trend and a phenomenon of hollowing ESV. (4) The spatial agglomeration effect of the ESV per unit area was continuously enhanced in Xining. The high-high (slope-altitude) type of area was distributed in the north and west of Xining, whereas the low-low type of area was distributed in the urban area at the intersection of major rivers in the southeast of Xining.
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