Different Approaches to Produce Transgenic Virus B Resistant Chrysanthemum
Chrysanthemum is a vegetative propagated culture in which viral transmission with planting material is important for its production. Chrysanthemum virus B (CVB) belongs to the viruses that strike this plant culture. Chrysanthemum virus B is found everywhere where chrysanthemum is cultivated. Damage...
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MDPI AG
2018-03-01
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| Series: | Agronomy |
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| Online Access: | http://www.mdpi.com/2073-4395/8/3/28 |
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| author | Tatiana Y. Mitiouchkina Aleksey P. Firsov Svetlana M. Titova Alexander S. Pushin Olga A. Shulga Sergey V. Dolgov |
| author_facet | Tatiana Y. Mitiouchkina Aleksey P. Firsov Svetlana M. Titova Alexander S. Pushin Olga A. Shulga Sergey V. Dolgov |
| author_sort | Tatiana Y. Mitiouchkina |
| collection | DOAJ |
| description | Chrysanthemum is a vegetative propagated culture in which viral transmission with planting material is important for its production. Chrysanthemum virus B (CVB) belongs to the viruses that strike this plant culture. Chrysanthemum virus B is found everywhere where chrysanthemum is cultivated. Damage to plants by CVB often leads to a complete loss of floral yield. Chrysanthemum (Chrysanthemum morifolium Ramat cv. White Snowdon) was transformed via Agrobacterium-mediated DNA delivery with the aim of improving resistance to CVB infection. Transformation vectors contain the nucleotide sequence of CVB coat proteins (CP) in sense, antisense, and double sense orientation. The transformative vectors also invert repeats of CVB coat protein gene fragments for the induction of RNA-interference. The transgenic chrysanthemum plants were successfully obtained. The integration of the target sequences in plant genomes was confirmed by polymerase chain reaction (PCR) and Southern blot analyses. Chrysanthemum lines were transformed with antisense, sense, and double sense CVB CP sequences, as well as with hairpin RNA-interference constructs that were assayed for resistance to CVB. Infection of transgenic plants by CVB through the grafting of infected scions shows resistance only among plants with carried double sense (16.7%) and hairpin (12.5%) constructs. The plants transformed by sense and double sense sequences were observed and classified as tolerant. |
| first_indexed | 2024-12-17T21:49:11Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-12-17T21:49:11Z |
| publishDate | 2018-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-88174b7df48e42b5841f703e6a9fd3662022-12-21T21:31:22ZengMDPI AGAgronomy2073-43952018-03-01832810.3390/agronomy8030028agronomy8030028Different Approaches to Produce Transgenic Virus B Resistant ChrysanthemumTatiana Y. Mitiouchkina0Aleksey P. Firsov1Svetlana M. Titova2Alexander S. Pushin3Olga A. Shulga4Sergey V. Dolgov5Nikita Botanical Garden, Yalta 298648, RussiaBranch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, The Russian Academy of Sciences, Pushchino, Moscow 142290, RussiaAll-Russian Research Institute of Agriculture Biotechnology, The Russian Academy of Sciences, TimirazevskajaStr.42, Moscow 127550, RussiaNikita Botanical Garden, Yalta 298648, RussiaAll-Russian Research Institute of Agriculture Biotechnology, The Russian Academy of Sciences, TimirazevskajaStr.42, Moscow 127550, RussiaNikita Botanical Garden, Yalta 298648, RussiaChrysanthemum is a vegetative propagated culture in which viral transmission with planting material is important for its production. Chrysanthemum virus B (CVB) belongs to the viruses that strike this plant culture. Chrysanthemum virus B is found everywhere where chrysanthemum is cultivated. Damage to plants by CVB often leads to a complete loss of floral yield. Chrysanthemum (Chrysanthemum morifolium Ramat cv. White Snowdon) was transformed via Agrobacterium-mediated DNA delivery with the aim of improving resistance to CVB infection. Transformation vectors contain the nucleotide sequence of CVB coat proteins (CP) in sense, antisense, and double sense orientation. The transformative vectors also invert repeats of CVB coat protein gene fragments for the induction of RNA-interference. The transgenic chrysanthemum plants were successfully obtained. The integration of the target sequences in plant genomes was confirmed by polymerase chain reaction (PCR) and Southern blot analyses. Chrysanthemum lines were transformed with antisense, sense, and double sense CVB CP sequences, as well as with hairpin RNA-interference constructs that were assayed for resistance to CVB. Infection of transgenic plants by CVB through the grafting of infected scions shows resistance only among plants with carried double sense (16.7%) and hairpin (12.5%) constructs. The plants transformed by sense and double sense sequences were observed and classified as tolerant.http://www.mdpi.com/2073-4395/8/3/28chrysanthemumvirus Bcoat proteinRNA-interferencetransformation |
| spellingShingle | Tatiana Y. Mitiouchkina Aleksey P. Firsov Svetlana M. Titova Alexander S. Pushin Olga A. Shulga Sergey V. Dolgov Different Approaches to Produce Transgenic Virus B Resistant Chrysanthemum Agronomy chrysanthemum virus B coat protein RNA-interference transformation |
| title | Different Approaches to Produce Transgenic Virus B Resistant Chrysanthemum |
| title_full | Different Approaches to Produce Transgenic Virus B Resistant Chrysanthemum |
| title_fullStr | Different Approaches to Produce Transgenic Virus B Resistant Chrysanthemum |
| title_full_unstemmed | Different Approaches to Produce Transgenic Virus B Resistant Chrysanthemum |
| title_short | Different Approaches to Produce Transgenic Virus B Resistant Chrysanthemum |
| title_sort | different approaches to produce transgenic virus b resistant chrysanthemum |
| topic | chrysanthemum virus B coat protein RNA-interference transformation |
| url | http://www.mdpi.com/2073-4395/8/3/28 |
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