In-depth sequence analysis of highly-conserved pyrH gene to study distributions of oral treponemes in periodontal disease versus health
Background: More than 75 species/species-level phylotypes of oral treponeme bacteria inhabit the oral cavity. However, their respective genomic compositions and clinical distributions remain poorly understood. Objectives: To compare distributions of phylogroup 1 and 2 oral treponemes in subjects wit...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2017-05-01
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| Series: | Journal of Oral Microbiology |
| Online Access: | http://dx.doi.org/10.1080/20002297.2017.1325210 |
| _version_ | 1817981477164417024 |
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| author | Rory M. Watt |
| author_facet | Rory M. Watt |
| author_sort | Rory M. Watt |
| collection | DOAJ |
| description | Background: More than 75 species/species-level phylotypes of oral treponeme bacteria inhabit the oral cavity. However, their respective genomic compositions and clinical distributions remain poorly understood. Objectives: To compare distributions of phylogroup 1 and 2 oral treponemes in subjects with various periodontal health conditions, via sequence analysis of a highly-conserved treponeme ‘housekeeping’ gene. Methods: Subgingival plaque samples were collected from Chinese subjects with chronic periodontitis (n=5), aggressive periodontitis (n=4), gingivitis (n=5), and healthy controls (n=4). Samples were analyzed by a PCR/plasmid clone sequencing-based approach, using primer sets targeting the pyrH gene. Data was analyzed using various computational/bioinformatic approaches. Results: 1,227 quality-filtered pyrH gene sequences were obtained (mean 66.2±9.6 sequences per subject), which were assigned to 33 ‘pyrH genotypes’ (97% identity cut-off). 538 pyrH sequences (17 pyrH genotypes) corresponded to phylogroup 1 treponemes (including ‘T. vincentii’, Treponema medium, and ‘Treponema sinensis’ taxa). 689 pyrH sequences (16 pyrH genotypes) corresponded to phylogroup 2 taxa. Correlations between pyrH genotype distributions and disease status were complex. One pyrH genotype, which was phylogenetically-related to T. denticola GM-1/MS25 strains, was highly prevalent: being detected in 17/18 subjects. Conclusions: Both healthy and periodontally-diseased subjects harbor multiple genetic lineages corresponding to the same treponeme species/phylotype within their subgingival niches. |
| first_indexed | 2024-04-13T23:06:18Z |
| format | Article |
| id | doaj.art-881a5d403fee43b38c07cd527a5000da |
| institution | Directory Open Access Journal |
| issn | 2000-2297 |
| language | English |
| last_indexed | 2024-04-13T23:06:18Z |
| publishDate | 2017-05-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Oral Microbiology |
| spelling | doaj.art-881a5d403fee43b38c07cd527a5000da2022-12-22T02:25:40ZengTaylor & Francis GroupJournal of Oral Microbiology2000-22972017-05-019010.1080/20002297.2017.13252101325210In-depth sequence analysis of highly-conserved pyrH gene to study distributions of oral treponemes in periodontal disease versus healthRory M. Watt0University of Hong KongBackground: More than 75 species/species-level phylotypes of oral treponeme bacteria inhabit the oral cavity. However, their respective genomic compositions and clinical distributions remain poorly understood. Objectives: To compare distributions of phylogroup 1 and 2 oral treponemes in subjects with various periodontal health conditions, via sequence analysis of a highly-conserved treponeme ‘housekeeping’ gene. Methods: Subgingival plaque samples were collected from Chinese subjects with chronic periodontitis (n=5), aggressive periodontitis (n=4), gingivitis (n=5), and healthy controls (n=4). Samples were analyzed by a PCR/plasmid clone sequencing-based approach, using primer sets targeting the pyrH gene. Data was analyzed using various computational/bioinformatic approaches. Results: 1,227 quality-filtered pyrH gene sequences were obtained (mean 66.2±9.6 sequences per subject), which were assigned to 33 ‘pyrH genotypes’ (97% identity cut-off). 538 pyrH sequences (17 pyrH genotypes) corresponded to phylogroup 1 treponemes (including ‘T. vincentii’, Treponema medium, and ‘Treponema sinensis’ taxa). 689 pyrH sequences (16 pyrH genotypes) corresponded to phylogroup 2 taxa. Correlations between pyrH genotype distributions and disease status were complex. One pyrH genotype, which was phylogenetically-related to T. denticola GM-1/MS25 strains, was highly prevalent: being detected in 17/18 subjects. Conclusions: Both healthy and periodontally-diseased subjects harbor multiple genetic lineages corresponding to the same treponeme species/phylotype within their subgingival niches.http://dx.doi.org/10.1080/20002297.2017.1325210 |
| spellingShingle | Rory M. Watt In-depth sequence analysis of highly-conserved pyrH gene to study distributions of oral treponemes in periodontal disease versus health Journal of Oral Microbiology |
| title | In-depth sequence analysis of highly-conserved pyrH gene to study distributions of oral treponemes in periodontal disease versus health |
| title_full | In-depth sequence analysis of highly-conserved pyrH gene to study distributions of oral treponemes in periodontal disease versus health |
| title_fullStr | In-depth sequence analysis of highly-conserved pyrH gene to study distributions of oral treponemes in periodontal disease versus health |
| title_full_unstemmed | In-depth sequence analysis of highly-conserved pyrH gene to study distributions of oral treponemes in periodontal disease versus health |
| title_short | In-depth sequence analysis of highly-conserved pyrH gene to study distributions of oral treponemes in periodontal disease versus health |
| title_sort | in depth sequence analysis of highly conserved pyrh gene to study distributions of oral treponemes in periodontal disease versus health |
| url | http://dx.doi.org/10.1080/20002297.2017.1325210 |
| work_keys_str_mv | AT rorymwatt indepthsequenceanalysisofhighlyconservedpyrhgenetostudydistributionsoforaltreponemesinperiodontaldiseaseversushealth |