Identification of Bifidobacterium Strains Isolated from Fecal Samples of Some Iranian Subjects Using 16SrRNA Gene Sequence Analysis and PCR-based Gene Specific Primers
For the first time in Iran 40 strains of Bifidobacterium were isolated from feces of Iranian subjects. By using phenotypic tests, 18 isolates were identified as Bifidobacterium longum, 10 as Bifidobacterium bifidum and one as Bifidobacterium catenolatum. In order to validate these results and also t...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Kerman University of Medical Sciences
2005-12-01
|
| Series: | Journal of Kerman University of Medical Sciences |
| Subjects: | |
| Online Access: | https://jkmu.kmu.ac.ir/article_32028_615236f0cc12f1da59b50901fe5a31ab.pdf |
| _version_ | 1827911776831799296 |
|---|---|
| author | M Khomeiri S.A Mortazavi H.B Ghoddusi A Khamessan D Ahmad F Shahidi |
| author_facet | M Khomeiri S.A Mortazavi H.B Ghoddusi A Khamessan D Ahmad F Shahidi |
| author_sort | M Khomeiri |
| collection | DOAJ |
| description | For the first time in Iran 40 strains of Bifidobacterium were isolated from feces of Iranian subjects. By using phenotypic tests, 18 isolates were identified as Bifidobacterium longum, 10 as Bifidobacterium bifidum and one as Bifidobacterium catenolatum. In order to validate these results and also to identify other isolates that had not been identified by phenotypic tests, two methods of PCR with genus-specific primers of Bif164f and Bif601r and 16SrRNA gene sequence analysis were applied. Results of PCR confirmed the obtained phenotypic identifications. Moreover by this method the 8 remaining strains were identified as Bifidobacterium species. Using sequencing 16SrRNA gene, 5 B. longum strains were identified that had different fermentation pattern from B. longum. Some new ribose negative Bifidobacterium longum strains were also identified. The obtained results present new strains of Bifidobacterium longum. |
| first_indexed | 2024-03-13T02:07:13Z |
| format | Article |
| id | doaj.art-881b2b93797549d69d0f2e4dd7b960df |
| institution | Directory Open Access Journal |
| issn | 2008-2843 |
| language | English |
| last_indexed | 2024-03-13T02:07:13Z |
| publishDate | 2005-12-01 |
| publisher | Kerman University of Medical Sciences |
| record_format | Article |
| series | Journal of Kerman University of Medical Sciences |
| spelling | doaj.art-881b2b93797549d69d0f2e4dd7b960df2023-07-01T05:41:53ZengKerman University of Medical SciencesJournal of Kerman University of Medical Sciences2008-28432005-12-01111213132028Identification of Bifidobacterium Strains Isolated from Fecal Samples of Some Iranian Subjects Using 16SrRNA Gene Sequence Analysis and PCR-based Gene Specific PrimersM Khomeiri0S.A Mortazavi1H.B Ghoddusi2A Khamessan3D Ahmad4F Shahidi5PhD. Student of Food Science and TechnologyProfessorAssociate Professor, Food Science and Technology Department, Agriculturl School, Mashhad University of Medical Sciences, Mashhad, IranMember of Animal Science Research Institute, Karaj, IranFaculty Member of INRS, CanadaAssociate Professor, Food Science and Technology Department, Agriculturl School, Mashhad University of Medical Sciences, Mashhad, IranFor the first time in Iran 40 strains of Bifidobacterium were isolated from feces of Iranian subjects. By using phenotypic tests, 18 isolates were identified as Bifidobacterium longum, 10 as Bifidobacterium bifidum and one as Bifidobacterium catenolatum. In order to validate these results and also to identify other isolates that had not been identified by phenotypic tests, two methods of PCR with genus-specific primers of Bif164f and Bif601r and 16SrRNA gene sequence analysis were applied. Results of PCR confirmed the obtained phenotypic identifications. Moreover by this method the 8 remaining strains were identified as Bifidobacterium species. Using sequencing 16SrRNA gene, 5 B. longum strains were identified that had different fermentation pattern from B. longum. Some new ribose negative Bifidobacterium longum strains were also identified. The obtained results present new strains of Bifidobacterium longum.https://jkmu.kmu.ac.ir/article_32028_615236f0cc12f1da59b50901fe5a31ab.pdfbifidobacterium16s rrnapcriranian strainsbif 164fbif 601ridentification |
| spellingShingle | M Khomeiri S.A Mortazavi H.B Ghoddusi A Khamessan D Ahmad F Shahidi Identification of Bifidobacterium Strains Isolated from Fecal Samples of Some Iranian Subjects Using 16SrRNA Gene Sequence Analysis and PCR-based Gene Specific Primers Journal of Kerman University of Medical Sciences bifidobacterium 16s rrna pcr iranian strains bif 164f bif 601r identification |
| title | Identification of Bifidobacterium Strains Isolated from Fecal Samples of Some Iranian Subjects Using 16SrRNA Gene Sequence Analysis and PCR-based Gene Specific Primers |
| title_full | Identification of Bifidobacterium Strains Isolated from Fecal Samples of Some Iranian Subjects Using 16SrRNA Gene Sequence Analysis and PCR-based Gene Specific Primers |
| title_fullStr | Identification of Bifidobacterium Strains Isolated from Fecal Samples of Some Iranian Subjects Using 16SrRNA Gene Sequence Analysis and PCR-based Gene Specific Primers |
| title_full_unstemmed | Identification of Bifidobacterium Strains Isolated from Fecal Samples of Some Iranian Subjects Using 16SrRNA Gene Sequence Analysis and PCR-based Gene Specific Primers |
| title_short | Identification of Bifidobacterium Strains Isolated from Fecal Samples of Some Iranian Subjects Using 16SrRNA Gene Sequence Analysis and PCR-based Gene Specific Primers |
| title_sort | identification of bifidobacterium strains isolated from fecal samples of some iranian subjects using 16srrna gene sequence analysis and pcr based gene specific primers |
| topic | bifidobacterium 16s rrna pcr iranian strains bif 164f bif 601r identification |
| url | https://jkmu.kmu.ac.ir/article_32028_615236f0cc12f1da59b50901fe5a31ab.pdf |
| work_keys_str_mv | AT mkhomeiri identificationofbifidobacteriumstrainsisolatedfromfecalsamplesofsomeiraniansubjectsusing16srrnagenesequenceanalysisandpcrbasedgenespecificprimers AT samortazavi identificationofbifidobacteriumstrainsisolatedfromfecalsamplesofsomeiraniansubjectsusing16srrnagenesequenceanalysisandpcrbasedgenespecificprimers AT hbghoddusi identificationofbifidobacteriumstrainsisolatedfromfecalsamplesofsomeiraniansubjectsusing16srrnagenesequenceanalysisandpcrbasedgenespecificprimers AT akhamessan identificationofbifidobacteriumstrainsisolatedfromfecalsamplesofsomeiraniansubjectsusing16srrnagenesequenceanalysisandpcrbasedgenespecificprimers AT dahmad identificationofbifidobacteriumstrainsisolatedfromfecalsamplesofsomeiraniansubjectsusing16srrnagenesequenceanalysisandpcrbasedgenespecificprimers AT fshahidi identificationofbifidobacteriumstrainsisolatedfromfecalsamplesofsomeiraniansubjectsusing16srrnagenesequenceanalysisandpcrbasedgenespecificprimers |