RAB31 in glioma‐derived endothelial cells promotes glioma cell invasion via extracellular vesicle‐mediated enrichment of MYO1C
Extracellular vesicles (EV), important messengers in intercellular communication, can load and transport various bioactive components and participate in different biological processes. We previously isolated glioma human endothelial cells (GhECs) and found that GhECs, rather than normal human brain...
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| Format: | Article |
| Language: | English |
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Wiley
2024-01-01
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| Series: | FEBS Open Bio |
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| Online Access: | https://doi.org/10.1002/2211-5463.13736 |
| _version_ | 1797367616870809600 |
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| author | Jinghao Suo Yuxin Wang Lin Wang Bojun Qiu Zhixing Wang An Yan Boqin Qiang Wei Han Xiaozhong Peng |
| author_facet | Jinghao Suo Yuxin Wang Lin Wang Bojun Qiu Zhixing Wang An Yan Boqin Qiang Wei Han Xiaozhong Peng |
| author_sort | Jinghao Suo |
| collection | DOAJ |
| description | Extracellular vesicles (EV), important messengers in intercellular communication, can load and transport various bioactive components and participate in different biological processes. We previously isolated glioma human endothelial cells (GhECs) and found that GhECs, rather than normal human brain endothelial cells (NhECs), exhibit specific enrichment of MYO1C into EVs and promote the migration of glioma cells. In this study, we explored the mechanism by which MYO1C is secreted into EVs. We report that such secretion is dependent on RAB31, RAB27B, and FAS. When expression of RAB31 increases, MYO1C is enriched in secretory EVs. Finally, we identified an EV export mechanism for MYO1C that promotes glioma cell invasion and is dependent on RAB31 in GhECs. In summary, our data indicate that the knockdown of RAB31 can reduce enrichment of MYO1C in extracellular vesicles, thereby attenuating the promotion of glioma cell invasion by GhEC‐EVs. |
| first_indexed | 2024-03-08T17:20:56Z |
| format | Article |
| id | doaj.art-881b6b529d154d3eb573f9217f7c9503 |
| institution | Directory Open Access Journal |
| issn | 2211-5463 |
| language | English |
| last_indexed | 2024-03-08T17:20:56Z |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj.art-881b6b529d154d3eb573f9217f7c95032024-01-03T05:28:43ZengWileyFEBS Open Bio2211-54632024-01-0114113814710.1002/2211-5463.13736RAB31 in glioma‐derived endothelial cells promotes glioma cell invasion via extracellular vesicle‐mediated enrichment of MYO1CJinghao Suo0Yuxin Wang1Lin Wang2Bojun Qiu3Zhixing Wang4An Yan5Boqin Qiang6Wei Han7Xiaozhong Peng8Department of Molecular Biology and Biochemistry, Institute of Basic Medical Sciences, Medical Primate Research Center, Neuroscience Center, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College Beijing ChinaDepartment of Molecular Biology and Biochemistry, Institute of Basic Medical Sciences, Medical Primate Research Center, Neuroscience Center, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College Beijing ChinaDepartment of Molecular Biology and Biochemistry, Institute of Basic Medical Sciences, Medical Primate Research Center, Neuroscience Center, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College Beijing ChinaDepartment of Molecular Biology and Biochemistry, Institute of Basic Medical Sciences, Medical Primate Research Center, Neuroscience Center, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College Beijing ChinaDepartment of Molecular Biology and Biochemistry, Institute of Basic Medical Sciences, Medical Primate Research Center, Neuroscience Center, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College Beijing ChinaDepartment of Molecular Biology and Biochemistry, Institute of Basic Medical Sciences, Medical Primate Research Center, Neuroscience Center, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College Beijing ChinaDepartment of Molecular Biology and Biochemistry, Institute of Basic Medical Sciences, Medical Primate Research Center, Neuroscience Center, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College Beijing ChinaDepartment of Molecular Biology and Biochemistry, Institute of Basic Medical Sciences, Medical Primate Research Center, Neuroscience Center, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College Beijing ChinaDepartment of Molecular Biology and Biochemistry, Institute of Basic Medical Sciences, Medical Primate Research Center, Neuroscience Center, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College Beijing ChinaExtracellular vesicles (EV), important messengers in intercellular communication, can load and transport various bioactive components and participate in different biological processes. We previously isolated glioma human endothelial cells (GhECs) and found that GhECs, rather than normal human brain endothelial cells (NhECs), exhibit specific enrichment of MYO1C into EVs and promote the migration of glioma cells. In this study, we explored the mechanism by which MYO1C is secreted into EVs. We report that such secretion is dependent on RAB31, RAB27B, and FAS. When expression of RAB31 increases, MYO1C is enriched in secretory EVs. Finally, we identified an EV export mechanism for MYO1C that promotes glioma cell invasion and is dependent on RAB31 in GhECs. In summary, our data indicate that the knockdown of RAB31 can reduce enrichment of MYO1C in extracellular vesicles, thereby attenuating the promotion of glioma cell invasion by GhEC‐EVs.https://doi.org/10.1002/2211-5463.13736endothelial cellsextracellular vesiclesgliomainvasionMYO1CRAB31 |
| spellingShingle | Jinghao Suo Yuxin Wang Lin Wang Bojun Qiu Zhixing Wang An Yan Boqin Qiang Wei Han Xiaozhong Peng RAB31 in glioma‐derived endothelial cells promotes glioma cell invasion via extracellular vesicle‐mediated enrichment of MYO1C FEBS Open Bio endothelial cells extracellular vesicles glioma invasion MYO1C RAB31 |
| title | RAB31 in glioma‐derived endothelial cells promotes glioma cell invasion via extracellular vesicle‐mediated enrichment of MYO1C |
| title_full | RAB31 in glioma‐derived endothelial cells promotes glioma cell invasion via extracellular vesicle‐mediated enrichment of MYO1C |
| title_fullStr | RAB31 in glioma‐derived endothelial cells promotes glioma cell invasion via extracellular vesicle‐mediated enrichment of MYO1C |
| title_full_unstemmed | RAB31 in glioma‐derived endothelial cells promotes glioma cell invasion via extracellular vesicle‐mediated enrichment of MYO1C |
| title_short | RAB31 in glioma‐derived endothelial cells promotes glioma cell invasion via extracellular vesicle‐mediated enrichment of MYO1C |
| title_sort | rab31 in glioma derived endothelial cells promotes glioma cell invasion via extracellular vesicle mediated enrichment of myo1c |
| topic | endothelial cells extracellular vesicles glioma invasion MYO1C RAB31 |
| url | https://doi.org/10.1002/2211-5463.13736 |
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