Respiratory syncytial virus in pediatric patients with severe acute respiratory infections in Senegal: findings from the 2022 sentinel surveillance season
Abstract In 2022, many regions around the world experienced a severe respiratory syncytial virus (RSV) epidemic with an earlier-than-usual start and increased numbers of paediatric patients in emergency departments. Here we carried out this study to describe the epidemiology and genetic characterist...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-47015-w |
| _version_ | 1797453221089771520 |
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| author | Mamadou Malado Jallow Moussa Moise Diagne Samba Niang Sagne Fatime Tall Jean Baptisse Niokhor Diouf Djibril Boiro Marie Pedapa Mendy Ndiendé Koba Ndiaye Davy Kiori Sara Sy Déborah Goudiaby Cheikh Loucoubar Gamou Fall Mamadou Aliou Barry Ndongo Dia |
| author_facet | Mamadou Malado Jallow Moussa Moise Diagne Samba Niang Sagne Fatime Tall Jean Baptisse Niokhor Diouf Djibril Boiro Marie Pedapa Mendy Ndiendé Koba Ndiaye Davy Kiori Sara Sy Déborah Goudiaby Cheikh Loucoubar Gamou Fall Mamadou Aliou Barry Ndongo Dia |
| author_sort | Mamadou Malado Jallow |
| collection | DOAJ |
| description | Abstract In 2022, many regions around the world experienced a severe respiratory syncytial virus (RSV) epidemic with an earlier-than-usual start and increased numbers of paediatric patients in emergency departments. Here we carried out this study to describe the epidemiology and genetic characteristics of RSV infection in patients hospitalized with severe acute respiratory infections in 2022. Samples were tested for RSV by multiplex real time reverse transcription polymerase chain reaction. Subsequently, a subset of RSV positive samples was selected for NGS sequencing. RSV was detected in 16.04%, among which RSV-A was confirmed in 7.5% and RSV-B in 76.7%. RSV infection were more identified in infants aged ≤ 11 months (83.3%) and a shift in the circulation pattern was observed, with highest incidences between September–November. Phylogenetic analyses revealed that all RSV-A strains belonged to GA2.3.5 genotype and all RSV-B strains to GB5.0.5a genotype. Three putative N-glycosylation sites at amino acid positions 103, 135, 237 were predicted among RSV-A strains, while four N-linked glycosylation sites at positions 81, 86, 231 and 294 were identified in RSV-B strains. Globally, our findings reveal an exclusive co-circulation of two genetic lineages of RSV within the pediatric population in Senegal, especially in infants aged ≤ 11 months. |
| first_indexed | 2024-03-09T15:19:39Z |
| format | Article |
| id | doaj.art-881bbbd369fa48d48e31986e5d4ba3b2 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-09T15:19:39Z |
| publishDate | 2023-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-881bbbd369fa48d48e31986e5d4ba3b22023-11-26T12:52:46ZengNature PortfolioScientific Reports2045-23222023-11-0113111210.1038/s41598-023-47015-wRespiratory syncytial virus in pediatric patients with severe acute respiratory infections in Senegal: findings from the 2022 sentinel surveillance seasonMamadou Malado Jallow0Moussa Moise Diagne1Samba Niang Sagne2Fatime Tall3Jean Baptisse Niokhor Diouf4Djibril Boiro5Marie Pedapa Mendy6Ndiendé Koba Ndiaye7Davy Kiori8Sara Sy9Déborah Goudiaby10Cheikh Loucoubar11Gamou Fall12Mamadou Aliou Barry13Ndongo Dia14Département de Virologie, Institut Pasteur de DakarDépartement de Virologie, Institut Pasteur de DakarInstitut Pasteur de Dakar, Unité d’Epidémiologie Des Maladies InfectieusesHôpital Des Enfants Albert Royer de FannHôpital Roi Baudoin de GuediawayeCentre Hospitalier Abass NdaoDépartement de Virologie, Institut Pasteur de DakarDépartement de Virologie, Institut Pasteur de DakarDépartement de Virologie, Institut Pasteur de DakarDépartement de Virologie, Institut Pasteur de DakarDépartement de Virologie, Institut Pasteur de DakarInstitut Pasteur de Dakar, Unité d’Epidémiologie Des Maladies InfectieusesDépartement de Virologie, Institut Pasteur de DakarInstitut Pasteur de Dakar, Unité d’Epidémiologie Des Maladies InfectieusesDépartement de Virologie, Institut Pasteur de DakarAbstract In 2022, many regions around the world experienced a severe respiratory syncytial virus (RSV) epidemic with an earlier-than-usual start and increased numbers of paediatric patients in emergency departments. Here we carried out this study to describe the epidemiology and genetic characteristics of RSV infection in patients hospitalized with severe acute respiratory infections in 2022. Samples were tested for RSV by multiplex real time reverse transcription polymerase chain reaction. Subsequently, a subset of RSV positive samples was selected for NGS sequencing. RSV was detected in 16.04%, among which RSV-A was confirmed in 7.5% and RSV-B in 76.7%. RSV infection were more identified in infants aged ≤ 11 months (83.3%) and a shift in the circulation pattern was observed, with highest incidences between September–November. Phylogenetic analyses revealed that all RSV-A strains belonged to GA2.3.5 genotype and all RSV-B strains to GB5.0.5a genotype. Three putative N-glycosylation sites at amino acid positions 103, 135, 237 were predicted among RSV-A strains, while four N-linked glycosylation sites at positions 81, 86, 231 and 294 were identified in RSV-B strains. Globally, our findings reveal an exclusive co-circulation of two genetic lineages of RSV within the pediatric population in Senegal, especially in infants aged ≤ 11 months.https://doi.org/10.1038/s41598-023-47015-w |
| spellingShingle | Mamadou Malado Jallow Moussa Moise Diagne Samba Niang Sagne Fatime Tall Jean Baptisse Niokhor Diouf Djibril Boiro Marie Pedapa Mendy Ndiendé Koba Ndiaye Davy Kiori Sara Sy Déborah Goudiaby Cheikh Loucoubar Gamou Fall Mamadou Aliou Barry Ndongo Dia Respiratory syncytial virus in pediatric patients with severe acute respiratory infections in Senegal: findings from the 2022 sentinel surveillance season Scientific Reports |
| title | Respiratory syncytial virus in pediatric patients with severe acute respiratory infections in Senegal: findings from the 2022 sentinel surveillance season |
| title_full | Respiratory syncytial virus in pediatric patients with severe acute respiratory infections in Senegal: findings from the 2022 sentinel surveillance season |
| title_fullStr | Respiratory syncytial virus in pediatric patients with severe acute respiratory infections in Senegal: findings from the 2022 sentinel surveillance season |
| title_full_unstemmed | Respiratory syncytial virus in pediatric patients with severe acute respiratory infections in Senegal: findings from the 2022 sentinel surveillance season |
| title_short | Respiratory syncytial virus in pediatric patients with severe acute respiratory infections in Senegal: findings from the 2022 sentinel surveillance season |
| title_sort | respiratory syncytial virus in pediatric patients with severe acute respiratory infections in senegal findings from the 2022 sentinel surveillance season |
| url | https://doi.org/10.1038/s41598-023-47015-w |
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