Liposome–trimethyl chitosan nanoparticles codeliver insulin and siVEGF to treat corneal alkali burns by inhibiting ferroptosis
Abstract Alkali burns are potentially blinding corneal injuries. Due to the lack of available effective therapies, the prognosis is poor. Thus, effective treatment methods for corneal alkali burns are urgently needed. Codelivery nanoparticles (NPs) with characteristics such as high bioavailability a...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-03-01
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| Series: | Bioengineering & Translational Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/btm2.10499 |
| _version_ | 1797870946859614208 |
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| author | Xiaojing Xiong Huiting Jiang Yukun Liao Yangrui Du Yu Zhang Zhigang Wang Minming Zheng Zhiyu Du |
| author_facet | Xiaojing Xiong Huiting Jiang Yukun Liao Yangrui Du Yu Zhang Zhigang Wang Minming Zheng Zhiyu Du |
| author_sort | Xiaojing Xiong |
| collection | DOAJ |
| description | Abstract Alkali burns are potentially blinding corneal injuries. Due to the lack of available effective therapies, the prognosis is poor. Thus, effective treatment methods for corneal alkali burns are urgently needed. Codelivery nanoparticles (NPs) with characteristics such as high bioavailability and few side effects have been considered effective therapeutic agents for ocular diseases. In this study, we designed a new combination therapy using liposomes and trimethyl chitosan (TMC) for the codelivery of insulin (INS) and vascular endothelial growth factor small interfering RNA (siVEGF) to treat alkali‐burned corneas. We describe the preparation and characterization of siVEGF‐TMC‐INS‐liposome (siVEGF‐TIL), drug release characteristics, intraocular tracing, pharmacodynamics, and biosafety. We found that siVEGF‐TIL could inhibit oxidative stress, inflammation, and the expression of VEGF in vitro and effectively maintained corneal transparency, accelerated epithelialization, and inhibited corneal neovascularization (CNV) in vivo. Morever, we found that the therapeutic mechanism of siVEGF‐TIL is possibly relevant to the inhibition of the ferroptosis signaling pathway by metabolomic analysis. In general, siVEGF‐TIL NPs could be a safe and effective therapy for corneal alkali burn. |
| first_indexed | 2024-04-10T00:36:29Z |
| format | Article |
| id | doaj.art-881fc8c9ec5b4cc090d898ee116ad955 |
| institution | Directory Open Access Journal |
| issn | 2380-6761 |
| language | English |
| last_indexed | 2024-04-10T00:36:29Z |
| publishDate | 2023-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Bioengineering & Translational Medicine |
| spelling | doaj.art-881fc8c9ec5b4cc090d898ee116ad9552023-03-14T16:53:48ZengWileyBioengineering & Translational Medicine2380-67612023-03-0182n/an/a10.1002/btm2.10499Liposome–trimethyl chitosan nanoparticles codeliver insulin and siVEGF to treat corneal alkali burns by inhibiting ferroptosisXiaojing Xiong0Huiting Jiang1Yukun Liao2Yangrui Du3Yu Zhang4Zhigang Wang5Minming Zheng6Zhiyu Du7Department of Ophthalmology Second Affiliated Hospital of Chongqing Medical University Chongqing ChinaDepartment of Ophthalmology Second Affiliated Hospital of Chongqing Medical University Chongqing ChinaDepartment of Ophthalmology Second Affiliated Hospital of Chongqing Medical University Chongqing ChinaDepartment of Ophthalmology Second Affiliated Hospital of Chongqing Medical University Chongqing ChinaDepartment of Ophthalmology Second Affiliated Hospital of Chongqing Medical University Chongqing ChinaChongqing Key Laboratory of Ultrasound Molecular Imaging Second Affiliated Hospital of Chongqing Medical University Chongqing ChinaDepartment of Ophthalmology Second Affiliated Hospital of Chongqing Medical University Chongqing ChinaDepartment of Ophthalmology Second Affiliated Hospital of Chongqing Medical University Chongqing ChinaAbstract Alkali burns are potentially blinding corneal injuries. Due to the lack of available effective therapies, the prognosis is poor. Thus, effective treatment methods for corneal alkali burns are urgently needed. Codelivery nanoparticles (NPs) with characteristics such as high bioavailability and few side effects have been considered effective therapeutic agents for ocular diseases. In this study, we designed a new combination therapy using liposomes and trimethyl chitosan (TMC) for the codelivery of insulin (INS) and vascular endothelial growth factor small interfering RNA (siVEGF) to treat alkali‐burned corneas. We describe the preparation and characterization of siVEGF‐TMC‐INS‐liposome (siVEGF‐TIL), drug release characteristics, intraocular tracing, pharmacodynamics, and biosafety. We found that siVEGF‐TIL could inhibit oxidative stress, inflammation, and the expression of VEGF in vitro and effectively maintained corneal transparency, accelerated epithelialization, and inhibited corneal neovascularization (CNV) in vivo. Morever, we found that the therapeutic mechanism of siVEGF‐TIL is possibly relevant to the inhibition of the ferroptosis signaling pathway by metabolomic analysis. In general, siVEGF‐TIL NPs could be a safe and effective therapy for corneal alkali burn.https://doi.org/10.1002/btm2.10499corneal alkali burnferroptosis inhibitioninsulinnanomedicinesiVEGF |
| spellingShingle | Xiaojing Xiong Huiting Jiang Yukun Liao Yangrui Du Yu Zhang Zhigang Wang Minming Zheng Zhiyu Du Liposome–trimethyl chitosan nanoparticles codeliver insulin and siVEGF to treat corneal alkali burns by inhibiting ferroptosis Bioengineering & Translational Medicine corneal alkali burn ferroptosis inhibition insulin nanomedicine siVEGF |
| title | Liposome–trimethyl chitosan nanoparticles codeliver insulin and siVEGF to treat corneal alkali burns by inhibiting ferroptosis |
| title_full | Liposome–trimethyl chitosan nanoparticles codeliver insulin and siVEGF to treat corneal alkali burns by inhibiting ferroptosis |
| title_fullStr | Liposome–trimethyl chitosan nanoparticles codeliver insulin and siVEGF to treat corneal alkali burns by inhibiting ferroptosis |
| title_full_unstemmed | Liposome–trimethyl chitosan nanoparticles codeliver insulin and siVEGF to treat corneal alkali burns by inhibiting ferroptosis |
| title_short | Liposome–trimethyl chitosan nanoparticles codeliver insulin and siVEGF to treat corneal alkali burns by inhibiting ferroptosis |
| title_sort | liposome trimethyl chitosan nanoparticles codeliver insulin and sivegf to treat corneal alkali burns by inhibiting ferroptosis |
| topic | corneal alkali burn ferroptosis inhibition insulin nanomedicine siVEGF |
| url | https://doi.org/10.1002/btm2.10499 |
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