Optical Manipulation of Fibroblasts with Femtosecond Pulse and CW Laser
Using tight focusing light, optical tweezers (OT) are tools that can manipulate and capture microscopic particles and biological cells as well as characterize a wide range of micro and nanomaterials. In this paper, we focused on fibroblasts, which are widely used in the biomedical area for a variety...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-03-01
|
| Series: | Photonics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2304-6732/11/3/248 |
| _version_ | 1797239665897504768 |
|---|---|
| author | Xia Zhang Yi Wu Siao Cai Guoying Feng |
| author_facet | Xia Zhang Yi Wu Siao Cai Guoying Feng |
| author_sort | Xia Zhang |
| collection | DOAJ |
| description | Using tight focusing light, optical tweezers (OT) are tools that can manipulate and capture microscopic particles and biological cells as well as characterize a wide range of micro and nanomaterials. In this paper, we focused on fibroblasts, which are widely used in the biomedical area for a variety of purposes, including promoting human wound healing and preventing the early proliferation of tumor cells. We first built an optical tweezer experimental platform, using an 808 nm continuous-wave laser as the capture light source, to confirm that the device can precisely control the movement of single or multiple particles as well as fibroblasts. Then, a 1030 nm femtosecond laser was employed as the capture light source to study the manipulation of microparticles and fibroblasts at different powers. Lastly, a protracted manipulation protocol was used to prevent the fibroblasts from adhering to the wall. This method can be used to isolate and precisely block adherent growth of fibroblasts in cell populations. This experimental result can be further extended to other biological cells. |
| first_indexed | 2024-04-24T17:55:09Z |
| format | Article |
| id | doaj.art-88234b8cf966439b911e670fec60ced4 |
| institution | Directory Open Access Journal |
| issn | 2304-6732 |
| language | English |
| last_indexed | 2024-04-24T17:55:09Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Photonics |
| spelling | doaj.art-88234b8cf966439b911e670fec60ced42024-03-27T14:00:09ZengMDPI AGPhotonics2304-67322024-03-0111324810.3390/photonics11030248Optical Manipulation of Fibroblasts with Femtosecond Pulse and CW LaserXia Zhang0Yi Wu1Siao Cai2Guoying Feng3Institute of Laser & Micro/Nano Engineering, Sichuan University, Chengdu 610065, ChinaInstitute of Laser & Micro/Nano Engineering, Sichuan University, Chengdu 610065, ChinaCollege of Computer Science, Sichuan University, Chengdu 610065, ChinaInstitute of Laser & Micro/Nano Engineering, Sichuan University, Chengdu 610065, ChinaUsing tight focusing light, optical tweezers (OT) are tools that can manipulate and capture microscopic particles and biological cells as well as characterize a wide range of micro and nanomaterials. In this paper, we focused on fibroblasts, which are widely used in the biomedical area for a variety of purposes, including promoting human wound healing and preventing the early proliferation of tumor cells. We first built an optical tweezer experimental platform, using an 808 nm continuous-wave laser as the capture light source, to confirm that the device can precisely control the movement of single or multiple particles as well as fibroblasts. Then, a 1030 nm femtosecond laser was employed as the capture light source to study the manipulation of microparticles and fibroblasts at different powers. Lastly, a protracted manipulation protocol was used to prevent the fibroblasts from adhering to the wall. This method can be used to isolate and precisely block adherent growth of fibroblasts in cell populations. This experimental result can be further extended to other biological cells.https://www.mdpi.com/2304-6732/11/3/248optical manipulationfibroblastsfemtosecond laseroptical trapping |
| spellingShingle | Xia Zhang Yi Wu Siao Cai Guoying Feng Optical Manipulation of Fibroblasts with Femtosecond Pulse and CW Laser Photonics optical manipulation fibroblasts femtosecond laser optical trapping |
| title | Optical Manipulation of Fibroblasts with Femtosecond Pulse and CW Laser |
| title_full | Optical Manipulation of Fibroblasts with Femtosecond Pulse and CW Laser |
| title_fullStr | Optical Manipulation of Fibroblasts with Femtosecond Pulse and CW Laser |
| title_full_unstemmed | Optical Manipulation of Fibroblasts with Femtosecond Pulse and CW Laser |
| title_short | Optical Manipulation of Fibroblasts with Femtosecond Pulse and CW Laser |
| title_sort | optical manipulation of fibroblasts with femtosecond pulse and cw laser |
| topic | optical manipulation fibroblasts femtosecond laser optical trapping |
| url | https://www.mdpi.com/2304-6732/11/3/248 |
| work_keys_str_mv | AT xiazhang opticalmanipulationoffibroblastswithfemtosecondpulseandcwlaser AT yiwu opticalmanipulationoffibroblastswithfemtosecondpulseandcwlaser AT siaocai opticalmanipulationoffibroblastswithfemtosecondpulseandcwlaser AT guoyingfeng opticalmanipulationoffibroblastswithfemtosecondpulseandcwlaser |