Impacts of Milking and Housing Environment on Milk Microbiota
The aim of the present study was to determine the effects of farming environments on microbiota in raw milk and to assess the relationship among microbes by 16S rRNA sequencing methods. Samples of raw milk, cow trough water, teat dip cup, teat, teat liner, dairy hall air, cowshed air, feces, feed, a...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-12-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/10/12/2339 |
| _version_ | 1827700485546573824 |
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| author | Bingyao Du Lu Meng Huimin Liu Nan Zheng Yangdong Zhang Xiaodong Guo Shengguo Zhao Fadi Li Jiaqi Wang |
| author_facet | Bingyao Du Lu Meng Huimin Liu Nan Zheng Yangdong Zhang Xiaodong Guo Shengguo Zhao Fadi Li Jiaqi Wang |
| author_sort | Bingyao Du |
| collection | DOAJ |
| description | The aim of the present study was to determine the effects of farming environments on microbiota in raw milk and to assess the relationship among microbes by 16S rRNA sequencing methods. Samples of raw milk, cow trough water, teat dip cup, teat, teat liner, dairy hall air, cowshed air, feces, feed, and bedding from two farms were collected. The two highest abundant bacterial groups of Moraxellaceae and Staphylococcaceae were found in milk and teat liner samples, respectively, at Zhengzhou farm, Henan Province. Moreover, the two highest abundant bacterial groups of Enterobacteriaceae and Moraxellaceae were found in milk and teat dip cup samples, respectively, at Qiqihar farm, Heilongjiang Province. Source Tracker analysis revealed that the teat liner and teat dip cup were the most important contributors of microbes in milk samples at Zhengzhou farm and Qiqihar farm, respectively, which could be attributed to the management level of the farm. Therefore, disinfection and cleaning procedures should be developed to improve the quality of raw milk. |
| first_indexed | 2024-03-10T14:13:47Z |
| format | Article |
| id | doaj.art-88256390ef224f8aaff9fbeab135ba74 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T14:13:47Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-88256390ef224f8aaff9fbeab135ba742023-11-20T23:58:52ZengMDPI AGAnimals2076-26152020-12-011012233910.3390/ani10122339Impacts of Milking and Housing Environment on Milk MicrobiotaBingyao Du0Lu Meng1Huimin Liu2Nan Zheng3Yangdong Zhang4Xiaodong Guo5Shengguo Zhao6Fadi Li7Jiaqi Wang8State Key Laboratory of Grassland Agro-Ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, ChinaKey Laboratory of Quality & Safety Control for Milk and Dairy Products of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaKey Laboratory of Quality & Safety Control for Milk and Dairy Products of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaKey Laboratory of Quality & Safety Control for Milk and Dairy Products of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaKey Laboratory of Quality & Safety Control for Milk and Dairy Products of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaKey Laboratory of Quality & Safety Control for Milk and Dairy Products of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaKey Laboratory of Quality & Safety Control for Milk and Dairy Products of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory of Grassland Agro-Ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, ChinaKey Laboratory of Quality & Safety Control for Milk and Dairy Products of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaThe aim of the present study was to determine the effects of farming environments on microbiota in raw milk and to assess the relationship among microbes by 16S rRNA sequencing methods. Samples of raw milk, cow trough water, teat dip cup, teat, teat liner, dairy hall air, cowshed air, feces, feed, and bedding from two farms were collected. The two highest abundant bacterial groups of Moraxellaceae and Staphylococcaceae were found in milk and teat liner samples, respectively, at Zhengzhou farm, Henan Province. Moreover, the two highest abundant bacterial groups of Enterobacteriaceae and Moraxellaceae were found in milk and teat dip cup samples, respectively, at Qiqihar farm, Heilongjiang Province. Source Tracker analysis revealed that the teat liner and teat dip cup were the most important contributors of microbes in milk samples at Zhengzhou farm and Qiqihar farm, respectively, which could be attributed to the management level of the farm. Therefore, disinfection and cleaning procedures should be developed to improve the quality of raw milk.https://www.mdpi.com/2076-2615/10/12/2339milkfarming environmentsmicrobiota16S rRNA gene sequencing |
| spellingShingle | Bingyao Du Lu Meng Huimin Liu Nan Zheng Yangdong Zhang Xiaodong Guo Shengguo Zhao Fadi Li Jiaqi Wang Impacts of Milking and Housing Environment on Milk Microbiota Animals milk farming environments microbiota 16S rRNA gene sequencing |
| title | Impacts of Milking and Housing Environment on Milk Microbiota |
| title_full | Impacts of Milking and Housing Environment on Milk Microbiota |
| title_fullStr | Impacts of Milking and Housing Environment on Milk Microbiota |
| title_full_unstemmed | Impacts of Milking and Housing Environment on Milk Microbiota |
| title_short | Impacts of Milking and Housing Environment on Milk Microbiota |
| title_sort | impacts of milking and housing environment on milk microbiota |
| topic | milk farming environments microbiota 16S rRNA gene sequencing |
| url | https://www.mdpi.com/2076-2615/10/12/2339 |
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