Genome editing in macroalgae: advances and challenges
This minireview examines the current state and challenges of genome editing in macroalgae. Despite the ecological and economic significance of this group of organisms, genome editing has seen limited applications. While CRISPR functionality has been established in two brown (Ectocarpus species 7 and...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2024-03-01
|
| Series: | Frontiers in Genome Editing |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fgeed.2024.1380682/full |
| _version_ | 1797270917703794688 |
|---|---|
| author | Jonas De Saeger Emma Coulembier Vandelannoote Emma Coulembier Vandelannoote Emma Coulembier Vandelannoote Hojun Lee Jihae Park Jonas Blomme Jonas Blomme Jonas Blomme |
| author_facet | Jonas De Saeger Emma Coulembier Vandelannoote Emma Coulembier Vandelannoote Emma Coulembier Vandelannoote Hojun Lee Jihae Park Jonas Blomme Jonas Blomme Jonas Blomme |
| author_sort | Jonas De Saeger |
| collection | DOAJ |
| description | This minireview examines the current state and challenges of genome editing in macroalgae. Despite the ecological and economic significance of this group of organisms, genome editing has seen limited applications. While CRISPR functionality has been established in two brown (Ectocarpus species 7 and Saccharina japonica) and one green seaweed (Ulva prolifera), these studies are limited to proof-of-concept demonstrations. All studies also (co)-targeted ADENINE PHOSPHORIBOSYL TRANSFERASE to enrich for mutants, due to the relatively low editing efficiencies. To advance the field, there should be a focus on advancing auxiliary technologies, particularly stable transformation, so that novel editing reagents can be screened for their efficiency. More work is also needed on understanding DNA repair in these organisms, as this is tightly linked with the editing outcomes. Developing efficient genome editing tools for macroalgae will unlock the ability to characterize their genes, which is largely uncharted terrain. Moreover, given their economic importance, genome editing will also impact breeding campaigns to develop strains that have better yields, produce more commercially valuable compounds, and show improved resilience to the impacts of global change. |
| first_indexed | 2024-04-25T02:11:53Z |
| format | Article |
| id | doaj.art-88271c365abd409a90cf8613bca8c3f2 |
| institution | Directory Open Access Journal |
| issn | 2673-3439 |
| language | English |
| last_indexed | 2024-04-25T02:11:53Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genome Editing |
| spelling | doaj.art-88271c365abd409a90cf8613bca8c3f22024-03-07T11:14:53ZengFrontiers Media S.A.Frontiers in Genome Editing2673-34392024-03-01610.3389/fgeed.2024.13806821380682Genome editing in macroalgae: advances and challengesJonas De Saeger0Emma Coulembier Vandelannoote1Emma Coulembier Vandelannoote2Emma Coulembier Vandelannoote3Hojun Lee4Jihae Park5Jonas Blomme6Jonas Blomme7Jonas Blomme8Bio Environmental Science and Technology (BEST) Lab, Ghent University Global Campus, Yeonsu-gu, Republic of KoreaDepartment of Biology, Phycology Research Group, Ghent University, Ghent, BelgiumDepartment of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, BelgiumVIB-UGent Center for Plant Systems Biology, Ghent, BelgiumBio Environmental Science and Technology (BEST) Lab, Ghent University Global Campus, Yeonsu-gu, Republic of KoreaBio Environmental Science and Technology (BEST) Lab, Ghent University Global Campus, Yeonsu-gu, Republic of KoreaDepartment of Biology, Phycology Research Group, Ghent University, Ghent, BelgiumDepartment of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, BelgiumVIB-UGent Center for Plant Systems Biology, Ghent, BelgiumThis minireview examines the current state and challenges of genome editing in macroalgae. Despite the ecological and economic significance of this group of organisms, genome editing has seen limited applications. While CRISPR functionality has been established in two brown (Ectocarpus species 7 and Saccharina japonica) and one green seaweed (Ulva prolifera), these studies are limited to proof-of-concept demonstrations. All studies also (co)-targeted ADENINE PHOSPHORIBOSYL TRANSFERASE to enrich for mutants, due to the relatively low editing efficiencies. To advance the field, there should be a focus on advancing auxiliary technologies, particularly stable transformation, so that novel editing reagents can be screened for their efficiency. More work is also needed on understanding DNA repair in these organisms, as this is tightly linked with the editing outcomes. Developing efficient genome editing tools for macroalgae will unlock the ability to characterize their genes, which is largely uncharted terrain. Moreover, given their economic importance, genome editing will also impact breeding campaigns to develop strains that have better yields, produce more commercially valuable compounds, and show improved resilience to the impacts of global change.https://www.frontiersin.org/articles/10.3389/fgeed.2024.1380682/fullgenome editingCRISPRmacroalgaeseaweed biotechnologyseaweed breedingreverse genetics |
| spellingShingle | Jonas De Saeger Emma Coulembier Vandelannoote Emma Coulembier Vandelannoote Emma Coulembier Vandelannoote Hojun Lee Jihae Park Jonas Blomme Jonas Blomme Jonas Blomme Genome editing in macroalgae: advances and challenges Frontiers in Genome Editing genome editing CRISPR macroalgae seaweed biotechnology seaweed breeding reverse genetics |
| title | Genome editing in macroalgae: advances and challenges |
| title_full | Genome editing in macroalgae: advances and challenges |
| title_fullStr | Genome editing in macroalgae: advances and challenges |
| title_full_unstemmed | Genome editing in macroalgae: advances and challenges |
| title_short | Genome editing in macroalgae: advances and challenges |
| title_sort | genome editing in macroalgae advances and challenges |
| topic | genome editing CRISPR macroalgae seaweed biotechnology seaweed breeding reverse genetics |
| url | https://www.frontiersin.org/articles/10.3389/fgeed.2024.1380682/full |
| work_keys_str_mv | AT jonasdesaeger genomeeditinginmacroalgaeadvancesandchallenges AT emmacoulembiervandelannoote genomeeditinginmacroalgaeadvancesandchallenges AT emmacoulembiervandelannoote genomeeditinginmacroalgaeadvancesandchallenges AT emmacoulembiervandelannoote genomeeditinginmacroalgaeadvancesandchallenges AT hojunlee genomeeditinginmacroalgaeadvancesandchallenges AT jihaepark genomeeditinginmacroalgaeadvancesandchallenges AT jonasblomme genomeeditinginmacroalgaeadvancesandchallenges AT jonasblomme genomeeditinginmacroalgaeadvancesandchallenges AT jonasblomme genomeeditinginmacroalgaeadvancesandchallenges |