Blood mRNA Expression Profiles of Autophagy, Apoptosis, and Hypoxia Markers on Blood Cardioplegia and Custodiol Cardioplegia Groups
Abstract Introduction: Blood cardioplegia (BC) and Custodiol cardioplegia (CC) have been used for a long time in open heart surgery and are highly effective solutions. The most controversial issue among these two is whether there is any difference between them regarding myocardial damage after isch...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sociedade Brasileira de Cirurgia Cardiovascular
2021-03-01
|
| Series: | Brazilian Journal of Cardiovascular Surgery |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-76382021000300331&tlng=en |
| _version_ | 1811246431311233024 |
|---|---|
| author | Deniz Elcik Aydın Tuncay Elif Funda Sener Serpil Taheri Reyhan Tahtasakal Ecmel Mehmetbeyoğlu Isın Gunes Omer Naci Emirogullari |
| author_facet | Deniz Elcik Aydın Tuncay Elif Funda Sener Serpil Taheri Reyhan Tahtasakal Ecmel Mehmetbeyoğlu Isın Gunes Omer Naci Emirogullari |
| author_sort | Deniz Elcik |
| collection | DOAJ |
| description | Abstract Introduction: Blood cardioplegia (BC) and Custodiol cardioplegia (CC) have been used for a long time in open heart surgery and are highly effective solutions. The most controversial issue among these two is whether there is any difference between them regarding myocardial damage after ischemia surgery. In this study, autophagy, apoptosis, and hypoxia markers were investigated and that way we evaluated the differences between BC and CC patients. Methods: A total of 30 patients were included in this study, using two different cardioplegic solutions. Three different whole blood samples of the patients were taken from a central vein (preoperatively, immediately postoperatively, and one day after surgery). Total ribonucleic acid was extracted from these samples. Quantitative real-time polymerase chain reaction was performed, and changes in gene expression were determined by the 2-∆∆Ct method of relative quantification. Results: In the CC group, Beclin gene expression level was found to be higher and this difference was statistically significant (P=0.0024). Similarly, cysteine-aspartic acid protease (caspase) 9 and hypoxia-inducible factor 1α messenger ribonucleic acid (mRNA) gene expression level increased and were significantly different in the CC group. In the BC group, Beclin and microtubule-associated protein light chain 3 expressions were higher in the samples taken one day after surgery. Caspases 3 and 8 gene expressions were significantly different in the BC group. Conclusion: As a result of the analysis performed between the two cardioplegia groups, it has been shown that CC harms the myocardium more than BC at the level of mRNA expression of related markers. |
| first_indexed | 2024-04-12T14:53:26Z |
| format | Article |
| id | doaj.art-8828ac23fb8849b8b1a678c1987d01fe |
| institution | Directory Open Access Journal |
| issn | 1678-9741 |
| language | English |
| last_indexed | 2024-04-12T14:53:26Z |
| publishDate | 2021-03-01 |
| publisher | Sociedade Brasileira de Cirurgia Cardiovascular |
| record_format | Article |
| series | Brazilian Journal of Cardiovascular Surgery |
| spelling | doaj.art-8828ac23fb8849b8b1a678c1987d01fe2022-12-22T03:28:20ZengSociedade Brasileira de Cirurgia CardiovascularBrazilian Journal of Cardiovascular Surgery1678-97412021-03-0136333133710.21470/1678-9741-2020-0330Blood mRNA Expression Profiles of Autophagy, Apoptosis, and Hypoxia Markers on Blood Cardioplegia and Custodiol Cardioplegia GroupsDeniz Elcikhttps://orcid.org/0000-0003-0992-1415Aydın TuncayElif Funda SenerSerpil TaheriReyhan TahtasakalEcmel MehmetbeyoğluIsın GunesOmer Naci EmirogullariAbstract Introduction: Blood cardioplegia (BC) and Custodiol cardioplegia (CC) have been used for a long time in open heart surgery and are highly effective solutions. The most controversial issue among these two is whether there is any difference between them regarding myocardial damage after ischemia surgery. In this study, autophagy, apoptosis, and hypoxia markers were investigated and that way we evaluated the differences between BC and CC patients. Methods: A total of 30 patients were included in this study, using two different cardioplegic solutions. Three different whole blood samples of the patients were taken from a central vein (preoperatively, immediately postoperatively, and one day after surgery). Total ribonucleic acid was extracted from these samples. Quantitative real-time polymerase chain reaction was performed, and changes in gene expression were determined by the 2-∆∆Ct method of relative quantification. Results: In the CC group, Beclin gene expression level was found to be higher and this difference was statistically significant (P=0.0024). Similarly, cysteine-aspartic acid protease (caspase) 9 and hypoxia-inducible factor 1α messenger ribonucleic acid (mRNA) gene expression level increased and were significantly different in the CC group. In the BC group, Beclin and microtubule-associated protein light chain 3 expressions were higher in the samples taken one day after surgery. Caspases 3 and 8 gene expressions were significantly different in the BC group. Conclusion: As a result of the analysis performed between the two cardioplegia groups, it has been shown that CC harms the myocardium more than BC at the level of mRNA expression of related markers.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-76382021000300331&tlng=enCardioplegic SolutionsRNA, Messenger, Caspase 9Aspartic AcidHeart Arrest,InducedCardiac Surgical ProceduresReal-Time Polymerase Chain Reaction |
| spellingShingle | Deniz Elcik Aydın Tuncay Elif Funda Sener Serpil Taheri Reyhan Tahtasakal Ecmel Mehmetbeyoğlu Isın Gunes Omer Naci Emirogullari Blood mRNA Expression Profiles of Autophagy, Apoptosis, and Hypoxia Markers on Blood Cardioplegia and Custodiol Cardioplegia Groups Brazilian Journal of Cardiovascular Surgery Cardioplegic Solutions RNA, Messenger, Caspase 9 Aspartic Acid Heart Arrest,Induced Cardiac Surgical Procedures Real-Time Polymerase Chain Reaction |
| title | Blood mRNA Expression Profiles of Autophagy, Apoptosis, and Hypoxia Markers on Blood Cardioplegia and Custodiol Cardioplegia Groups |
| title_full | Blood mRNA Expression Profiles of Autophagy, Apoptosis, and Hypoxia Markers on Blood Cardioplegia and Custodiol Cardioplegia Groups |
| title_fullStr | Blood mRNA Expression Profiles of Autophagy, Apoptosis, and Hypoxia Markers on Blood Cardioplegia and Custodiol Cardioplegia Groups |
| title_full_unstemmed | Blood mRNA Expression Profiles of Autophagy, Apoptosis, and Hypoxia Markers on Blood Cardioplegia and Custodiol Cardioplegia Groups |
| title_short | Blood mRNA Expression Profiles of Autophagy, Apoptosis, and Hypoxia Markers on Blood Cardioplegia and Custodiol Cardioplegia Groups |
| title_sort | blood mrna expression profiles of autophagy apoptosis and hypoxia markers on blood cardioplegia and custodiol cardioplegia groups |
| topic | Cardioplegic Solutions RNA, Messenger, Caspase 9 Aspartic Acid Heart Arrest,Induced Cardiac Surgical Procedures Real-Time Polymerase Chain Reaction |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-76382021000300331&tlng=en |
| work_keys_str_mv | AT denizelcik bloodmrnaexpressionprofilesofautophagyapoptosisandhypoxiamarkersonbloodcardioplegiaandcustodiolcardioplegiagroups AT aydıntuncay bloodmrnaexpressionprofilesofautophagyapoptosisandhypoxiamarkersonbloodcardioplegiaandcustodiolcardioplegiagroups AT eliffundasener bloodmrnaexpressionprofilesofautophagyapoptosisandhypoxiamarkersonbloodcardioplegiaandcustodiolcardioplegiagroups AT serpiltaheri bloodmrnaexpressionprofilesofautophagyapoptosisandhypoxiamarkersonbloodcardioplegiaandcustodiolcardioplegiagroups AT reyhantahtasakal bloodmrnaexpressionprofilesofautophagyapoptosisandhypoxiamarkersonbloodcardioplegiaandcustodiolcardioplegiagroups AT ecmelmehmetbeyoglu bloodmrnaexpressionprofilesofautophagyapoptosisandhypoxiamarkersonbloodcardioplegiaandcustodiolcardioplegiagroups AT isıngunes bloodmrnaexpressionprofilesofautophagyapoptosisandhypoxiamarkersonbloodcardioplegiaandcustodiolcardioplegiagroups AT omernaciemirogullari bloodmrnaexpressionprofilesofautophagyapoptosisandhypoxiamarkersonbloodcardioplegiaandcustodiolcardioplegiagroups |