Leukemia Cell Lines: In Vitro Models for the Study of Chronic Neutrophilic Leukemia
Chronic neutrophilic leukemia (CNL) is a rare myeloproliferative neoplasm that is genetically characterized by the absence of both the Philadelphia chromosome and BCR-ABL1 fusion gene and the high prevalence of mutations in the colony-stimulating factor 3 receptor (CSF3R). Additional disease-modifyi...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-05-01
|
| Series: | Current Oncology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1718-7729/28/3/166 |
| _version_ | 1797520756456816640 |
|---|---|
| author | Hans G. Drexler Stefan Nagel Hilmar Quentmeier |
| author_facet | Hans G. Drexler Stefan Nagel Hilmar Quentmeier |
| author_sort | Hans G. Drexler |
| collection | DOAJ |
| description | Chronic neutrophilic leukemia (CNL) is a rare myeloproliferative neoplasm that is genetically characterized by the absence of both the Philadelphia chromosome and BCR-ABL1 fusion gene and the high prevalence of mutations in the colony-stimulating factor 3 receptor (CSF3R). Additional disease-modifying mutations have been recognized in CNL samples, portraying a distinct mutational landscape. Despite the growing knowledge base on genomic aberrations, further progress could be gained from the availability of representative models of CNL. To address this gap, we screened a large panel of available leukemia cell lines, followed by a detailed mutational investigation with focus on the CNL-associated candidate driver genes. The sister cell lines CNLBC-1 and MOLM-20 were derived from a patient with CNL and carry CNL-typical molecular hallmarks, namely mutations in several genes, such as CSF3R, ASXL1, EZH2, NRAS, and SETBP1. The use of these validated and comprehensively characterized models will benefit the understanding of the pathobiology of CNL and help inform therapeutic strategies. |
| first_indexed | 2024-03-10T08:01:08Z |
| format | Article |
| id | doaj.art-8828deb130924eedbe201cca04f88164 |
| institution | Directory Open Access Journal |
| issn | 1198-0052 1718-7729 |
| language | English |
| last_indexed | 2024-03-10T08:01:08Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Current Oncology |
| spelling | doaj.art-8828deb130924eedbe201cca04f881642023-11-22T11:27:19ZengMDPI AGCurrent Oncology1198-00521718-77292021-05-012831790179410.3390/curroncol28030166Leukemia Cell Lines: In Vitro Models for the Study of Chronic Neutrophilic LeukemiaHans G. Drexler0Stefan Nagel1Hilmar Quentmeier2Faculty of Life Sciences, Technical University of Braunschweig, 38106 Braunschweig, GermanyDepartment of Human and Animal Cell Lines, Leibniz-Institute DSMZ-German Collection of Microorganisms and Cell Cultures, 38124 Braunschweig, GermanyDepartment of Human and Animal Cell Lines, Leibniz-Institute DSMZ-German Collection of Microorganisms and Cell Cultures, 38124 Braunschweig, GermanyChronic neutrophilic leukemia (CNL) is a rare myeloproliferative neoplasm that is genetically characterized by the absence of both the Philadelphia chromosome and BCR-ABL1 fusion gene and the high prevalence of mutations in the colony-stimulating factor 3 receptor (CSF3R). Additional disease-modifying mutations have been recognized in CNL samples, portraying a distinct mutational landscape. Despite the growing knowledge base on genomic aberrations, further progress could be gained from the availability of representative models of CNL. To address this gap, we screened a large panel of available leukemia cell lines, followed by a detailed mutational investigation with focus on the CNL-associated candidate driver genes. The sister cell lines CNLBC-1 and MOLM-20 were derived from a patient with CNL and carry CNL-typical molecular hallmarks, namely mutations in several genes, such as CSF3R, ASXL1, EZH2, NRAS, and SETBP1. The use of these validated and comprehensively characterized models will benefit the understanding of the pathobiology of CNL and help inform therapeutic strategies.https://www.mdpi.com/1718-7729/28/3/166cell linesCNLleukemiamodel |
| spellingShingle | Hans G. Drexler Stefan Nagel Hilmar Quentmeier Leukemia Cell Lines: In Vitro Models for the Study of Chronic Neutrophilic Leukemia Current Oncology cell lines CNL leukemia model |
| title | Leukemia Cell Lines: In Vitro Models for the Study of Chronic Neutrophilic Leukemia |
| title_full | Leukemia Cell Lines: In Vitro Models for the Study of Chronic Neutrophilic Leukemia |
| title_fullStr | Leukemia Cell Lines: In Vitro Models for the Study of Chronic Neutrophilic Leukemia |
| title_full_unstemmed | Leukemia Cell Lines: In Vitro Models for the Study of Chronic Neutrophilic Leukemia |
| title_short | Leukemia Cell Lines: In Vitro Models for the Study of Chronic Neutrophilic Leukemia |
| title_sort | leukemia cell lines in vitro models for the study of chronic neutrophilic leukemia |
| topic | cell lines CNL leukemia model |
| url | https://www.mdpi.com/1718-7729/28/3/166 |
| work_keys_str_mv | AT hansgdrexler leukemiacelllinesinvitromodelsforthestudyofchronicneutrophilicleukemia AT stefannagel leukemiacelllinesinvitromodelsforthestudyofchronicneutrophilicleukemia AT hilmarquentmeier leukemiacelllinesinvitromodelsforthestudyofchronicneutrophilicleukemia |