Profiling and Distribution of Metabolites of Procyanidin B2 in Mice by UPLC-DAD-ESI-IT-TOF-MSn Technique
The metabolite profiles and distributions of procyanidin B2 were qualitatively described using UPLC-DAD-ESI-IT-TOF-MSn without help of reference standards, and a possible metabolic pathway was proposed in the present study. Summarily, 53 metabolites (24 new metabolites) were detected as metabolites...
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| Format: | Article |
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Frontiers Media S.A.
2017-05-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fphar.2017.00231/full |
| _version_ | 1818650472174583808 |
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| author | Ying Xiao Zhongzhi Hu Zhiting Yin Yiming Zhou Taiyi Liu Xiaoli Zhou Dawei Chang |
| author_facet | Ying Xiao Zhongzhi Hu Zhiting Yin Yiming Zhou Taiyi Liu Xiaoli Zhou Dawei Chang |
| author_sort | Ying Xiao |
| collection | DOAJ |
| description | The metabolite profiles and distributions of procyanidin B2 were qualitatively described using UPLC-DAD-ESI-IT-TOF-MSn without help of reference standards, and a possible metabolic pathway was proposed in the present study. Summarily, 53 metabolites (24 new metabolites) were detected as metabolites of procyanidin B2, and 45 of them were tentatively identified. Twenty seven metabolites were assigned as similar metabolites of (−)-epicatechin by scission of the flavanol interflavanic bond C4–C8, including 16 aromatic metabolites, 5 conjugated metabolites, 3 ring-cleavage metabolites, and 2 phenylvalerolactone metabolites. Additionally, 14 metabolites were conjugates of free procyanidin B2, comprising 9 methylation metabolites, 8 sulfation metabolites, 5 hydration metabolites, 2 hydroxylation metabolites, 1 hydrogenation metabolites, and 1 glucuronidation metabolites. The results of metabolite distributions in organs indicated that the conjugated reaction of free procyanidin B2 mainly occurred in liver and diversified metabolites forms were observed in small intestine. The metabolic components of procyanidin B2 identified in mice provided useful information for further study of the bioactivity and mechanism of its action. |
| first_indexed | 2024-12-17T01:50:45Z |
| format | Article |
| id | doaj.art-882c6db02291450b99c85a5cd883bb97 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-17T01:50:45Z |
| publishDate | 2017-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-882c6db02291450b99c85a5cd883bb972022-12-21T22:08:05ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122017-05-01810.3389/fphar.2017.00231259837Profiling and Distribution of Metabolites of Procyanidin B2 in Mice by UPLC-DAD-ESI-IT-TOF-MSn TechniqueYing Xiao0Zhongzhi Hu1Zhiting Yin2Yiming Zhou3Taiyi Liu4Xiaoli Zhou5Dawei Chang6School of Perfume and Aroma Technology, Shanghai Institute of TechnologyShanghai, ChinaSchool of Perfume and Aroma Technology, Shanghai Institute of TechnologyShanghai, ChinaSchool of Perfume and Aroma Technology, Shanghai Institute of TechnologyShanghai, ChinaSchool of Perfume and Aroma Technology, Shanghai Institute of TechnologyShanghai, ChinaSchool of Perfume and Aroma Technology, Shanghai Institute of TechnologyShanghai, ChinaSchool of Perfume and Aroma Technology, Shanghai Institute of TechnologyShanghai, ChinaSchool of Food and Biological Engineering, Shaanxi University of Science and TechnologyXi'an, ChinaThe metabolite profiles and distributions of procyanidin B2 were qualitatively described using UPLC-DAD-ESI-IT-TOF-MSn without help of reference standards, and a possible metabolic pathway was proposed in the present study. Summarily, 53 metabolites (24 new metabolites) were detected as metabolites of procyanidin B2, and 45 of them were tentatively identified. Twenty seven metabolites were assigned as similar metabolites of (−)-epicatechin by scission of the flavanol interflavanic bond C4–C8, including 16 aromatic metabolites, 5 conjugated metabolites, 3 ring-cleavage metabolites, and 2 phenylvalerolactone metabolites. Additionally, 14 metabolites were conjugates of free procyanidin B2, comprising 9 methylation metabolites, 8 sulfation metabolites, 5 hydration metabolites, 2 hydroxylation metabolites, 1 hydrogenation metabolites, and 1 glucuronidation metabolites. The results of metabolite distributions in organs indicated that the conjugated reaction of free procyanidin B2 mainly occurred in liver and diversified metabolites forms were observed in small intestine. The metabolic components of procyanidin B2 identified in mice provided useful information for further study of the bioactivity and mechanism of its action.http://journal.frontiersin.org/article/10.3389/fphar.2017.00231/fullprocyanidin B2metabolic profileidentificationdistributionUPLC-DAD-ESI -IT-TOF-MSn |
| spellingShingle | Ying Xiao Zhongzhi Hu Zhiting Yin Yiming Zhou Taiyi Liu Xiaoli Zhou Dawei Chang Profiling and Distribution of Metabolites of Procyanidin B2 in Mice by UPLC-DAD-ESI-IT-TOF-MSn Technique Frontiers in Pharmacology procyanidin B2 metabolic profile identification distribution UPLC-DAD-ESI -IT-TOF-MSn |
| title | Profiling and Distribution of Metabolites of Procyanidin B2 in Mice by UPLC-DAD-ESI-IT-TOF-MSn Technique |
| title_full | Profiling and Distribution of Metabolites of Procyanidin B2 in Mice by UPLC-DAD-ESI-IT-TOF-MSn Technique |
| title_fullStr | Profiling and Distribution of Metabolites of Procyanidin B2 in Mice by UPLC-DAD-ESI-IT-TOF-MSn Technique |
| title_full_unstemmed | Profiling and Distribution of Metabolites of Procyanidin B2 in Mice by UPLC-DAD-ESI-IT-TOF-MSn Technique |
| title_short | Profiling and Distribution of Metabolites of Procyanidin B2 in Mice by UPLC-DAD-ESI-IT-TOF-MSn Technique |
| title_sort | profiling and distribution of metabolites of procyanidin b2 in mice by uplc dad esi it tof msn technique |
| topic | procyanidin B2 metabolic profile identification distribution UPLC-DAD-ESI -IT-TOF-MSn |
| url | http://journal.frontiersin.org/article/10.3389/fphar.2017.00231/full |
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