Development of a Highly Proliferated Bilayer Coating on 316L Stainless Steel Implants
In this research, a bilayer coating has been applied on the surface of 316 L stainless steel (316LSS) to provide highly proliferated metallic implants for bone regeneration. The first layer was prepared using electrophoretic deposition of graphene oxide (GO), while the top layer was coated utilizing...
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| Format: | Article |
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MDPI AG
2020-05-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/12/5/1022 |
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| author | Fatemeh Khosravi Saied Nouri Khorasani Shahla Khalili Rasoul Esmaeely Neisiany Erfan Rezvani Ghomi Fatemeh Ejeian Oisik Das Mohammad Hossein Nasr-Esfahani |
| author_facet | Fatemeh Khosravi Saied Nouri Khorasani Shahla Khalili Rasoul Esmaeely Neisiany Erfan Rezvani Ghomi Fatemeh Ejeian Oisik Das Mohammad Hossein Nasr-Esfahani |
| author_sort | Fatemeh Khosravi |
| collection | DOAJ |
| description | In this research, a bilayer coating has been applied on the surface of 316 L stainless steel (316LSS) to provide highly proliferated metallic implants for bone regeneration. The first layer was prepared using electrophoretic deposition of graphene oxide (GO), while the top layer was coated utilizing electrospinning of poly (ε-caprolactone) (PCL)/gelatin (Ge)/forsterite solutions. The morphology, porosity, wettability, biodegradability, bioactivity, cell attachment and cell viability of the prepared coatings were evaluated. The Field Emission Scanning Electron Microscopy (FESEM) results revealed the formation of uniform, continuous, and bead-free nanofibers. The Energy Dispersive X-ray (EDS) results confirmed well-distributed forsterite nanoparticles in the structure of the top coating. The porosity of the electrospun nanofibers was found to be above 70%. The water contact angle measurements indicated an improvement in the wettability of the coating by increasing the amount of nanoparticles. Furthermore, the electrospun nanofibers containing 1 and 3 wt.% of forsterite nanoparticles showed significant bioactivity after soaking in the simulated body fluid (SBF) solution for 21 days. In addition, to investigate the in vitro analysis, the MG-63 cells were cultured on the PCL/Ge/forsterite and GO-PCL/Ge/forsterite coatings. The results confirmed an excellent cell adhesion along with considerable cell growth and proliferation. It should be also noted that the existence of the forsterite nanoparticles and the GO layer substantially enhanced the cell proliferation of the coatings. |
| first_indexed | 2024-03-10T20:06:37Z |
| format | Article |
| id | doaj.art-882f2374d95343509ed54fa91786fe95 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T20:06:37Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-882f2374d95343509ed54fa91786fe952023-11-19T23:15:08ZengMDPI AGPolymers2073-43602020-05-01125102210.3390/polym12051022Development of a Highly Proliferated Bilayer Coating on 316L Stainless Steel ImplantsFatemeh Khosravi0Saied Nouri Khorasani1Shahla Khalili2Rasoul Esmaeely Neisiany3Erfan Rezvani Ghomi4Fatemeh Ejeian5Oisik Das6Mohammad Hossein Nasr-Esfahani7Department of Chemical Engineering, Isfahan University of Technology, Isfahan 8415683111, IranDepartment of Chemical Engineering, Isfahan University of Technology, Isfahan 8415683111, IranDepartment of Chemical Engineering, Isfahan University of Technology, Isfahan 8415683111, IranDepartment of Materials and Polymer Engineering, Faculty of Engineering, Hakim Sabzevari University, Sabzevar 9617976487, IranDepartment of Mechanical Engineering, Center for Nanofibers and Nanotechnology, National University of Singapore, Singapore 119260, SingaporeDepartment of Cellular Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan 8159358686, IranMaterial Science Division, Department of Engineering Sciences and Mathematics, Luleå University of Technology, 97187 Luleå, SwedenDepartment of Cellular Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan 8159358686, IranIn this research, a bilayer coating has been applied on the surface of 316 L stainless steel (316LSS) to provide highly proliferated metallic implants for bone regeneration. The first layer was prepared using electrophoretic deposition of graphene oxide (GO), while the top layer was coated utilizing electrospinning of poly (ε-caprolactone) (PCL)/gelatin (Ge)/forsterite solutions. The morphology, porosity, wettability, biodegradability, bioactivity, cell attachment and cell viability of the prepared coatings were evaluated. The Field Emission Scanning Electron Microscopy (FESEM) results revealed the formation of uniform, continuous, and bead-free nanofibers. The Energy Dispersive X-ray (EDS) results confirmed well-distributed forsterite nanoparticles in the structure of the top coating. The porosity of the electrospun nanofibers was found to be above 70%. The water contact angle measurements indicated an improvement in the wettability of the coating by increasing the amount of nanoparticles. Furthermore, the electrospun nanofibers containing 1 and 3 wt.% of forsterite nanoparticles showed significant bioactivity after soaking in the simulated body fluid (SBF) solution for 21 days. In addition, to investigate the in vitro analysis, the MG-63 cells were cultured on the PCL/Ge/forsterite and GO-PCL/Ge/forsterite coatings. The results confirmed an excellent cell adhesion along with considerable cell growth and proliferation. It should be also noted that the existence of the forsterite nanoparticles and the GO layer substantially enhanced the cell proliferation of the coatings.https://www.mdpi.com/2073-4360/12/5/1022biocompositesnanofiberselectrospinningcell culturegraphene oxide |
| spellingShingle | Fatemeh Khosravi Saied Nouri Khorasani Shahla Khalili Rasoul Esmaeely Neisiany Erfan Rezvani Ghomi Fatemeh Ejeian Oisik Das Mohammad Hossein Nasr-Esfahani Development of a Highly Proliferated Bilayer Coating on 316L Stainless Steel Implants Polymers biocomposites nanofibers electrospinning cell culture graphene oxide |
| title | Development of a Highly Proliferated Bilayer Coating on 316L Stainless Steel Implants |
| title_full | Development of a Highly Proliferated Bilayer Coating on 316L Stainless Steel Implants |
| title_fullStr | Development of a Highly Proliferated Bilayer Coating on 316L Stainless Steel Implants |
| title_full_unstemmed | Development of a Highly Proliferated Bilayer Coating on 316L Stainless Steel Implants |
| title_short | Development of a Highly Proliferated Bilayer Coating on 316L Stainless Steel Implants |
| title_sort | development of a highly proliferated bilayer coating on 316l stainless steel implants |
| topic | biocomposites nanofibers electrospinning cell culture graphene oxide |
| url | https://www.mdpi.com/2073-4360/12/5/1022 |
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