Development of an immunochromatographic test strip for the detection of procaine in milk
Procaine (PRC) is a local anaesthetic used in the treatment of several disease related to ageing, asthma, and arthritis. In this study, we describe a rapid, sensitive, simple, and accurate method for the determination of PRC in milk, using an immunochromatographic test strip. For the immunochromatog...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-01-01
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| Series: | Food and Agricultural Immunology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09540105.2018.1523371 |
| _version_ | 1818827043529293824 |
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| author | Kai Hao Steven Suryoprabowo Shanshan Song Liqiang Liu Qiankun Zheng Hua Kuang |
| author_facet | Kai Hao Steven Suryoprabowo Shanshan Song Liqiang Liu Qiankun Zheng Hua Kuang |
| author_sort | Kai Hao |
| collection | DOAJ |
| description | Procaine (PRC) is a local anaesthetic used in the treatment of several disease related to ageing, asthma, and arthritis. In this study, we describe a rapid, sensitive, simple, and accurate method for the determination of PRC in milk, using an immunochromatographic test strip. For the immunochromatographic test, antigen (PRC-bovine serum albumin) and goat anti-mouse IgG were drawn onto a nitrocellulose membrane representing the control line and test line. The test was developed with a sensitive monoclonal antibody specific for PRC, generated by immunizing BALB/c mice with a well-characterized PRC- Keyhole limpet hemocyanin conjugate, produced in our laboratory. Under optimized conditions, the semi-quantitative test strip was able to detect PRC at concentrations down to 1 ng/mL in both 0.01 M phosphate buffer saline (pH 7.4) and milk samples. All results were obtained within 5 min. Our data indicate that the immunochromatographic assay is capable of sensitive, rapid, and specific on-site screening of PRC. |
| first_indexed | 2024-12-19T00:37:17Z |
| format | Article |
| id | doaj.art-8831916419284c59ae4b6b197db87121 |
| institution | Directory Open Access Journal |
| issn | 0954-0105 1465-3443 |
| language | English |
| last_indexed | 2024-12-19T00:37:17Z |
| publishDate | 2018-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Food and Agricultural Immunology |
| spelling | doaj.art-8831916419284c59ae4b6b197db871212022-12-21T20:44:43ZengTaylor & Francis GroupFood and Agricultural Immunology0954-01051465-34432018-01-012911150116110.1080/09540105.2018.15233711523371Development of an immunochromatographic test strip for the detection of procaine in milkKai Hao0Steven Suryoprabowo1Shanshan Song2Liqiang Liu3Qiankun Zheng4Hua Kuang5School of Agricultural Engineering and Food Science, Shandong University of TechnologyState Key Laboratory of Food Science and Technology, Jiangnan UniversityState Key Laboratory of Food Science and Technology, Jiangnan UniversityState Key Laboratory of Food Science and Technology, Jiangnan UniversityDelishi GroupState Key Laboratory of Food Science and Technology, Jiangnan UniversityProcaine (PRC) is a local anaesthetic used in the treatment of several disease related to ageing, asthma, and arthritis. In this study, we describe a rapid, sensitive, simple, and accurate method for the determination of PRC in milk, using an immunochromatographic test strip. For the immunochromatographic test, antigen (PRC-bovine serum albumin) and goat anti-mouse IgG were drawn onto a nitrocellulose membrane representing the control line and test line. The test was developed with a sensitive monoclonal antibody specific for PRC, generated by immunizing BALB/c mice with a well-characterized PRC- Keyhole limpet hemocyanin conjugate, produced in our laboratory. Under optimized conditions, the semi-quantitative test strip was able to detect PRC at concentrations down to 1 ng/mL in both 0.01 M phosphate buffer saline (pH 7.4) and milk samples. All results were obtained within 5 min. Our data indicate that the immunochromatographic assay is capable of sensitive, rapid, and specific on-site screening of PRC.http://dx.doi.org/10.1080/09540105.2018.1523371immunochromatographic assaylocal anaestheticmonoclonal antibodyprocainestrip test |
| spellingShingle | Kai Hao Steven Suryoprabowo Shanshan Song Liqiang Liu Qiankun Zheng Hua Kuang Development of an immunochromatographic test strip for the detection of procaine in milk Food and Agricultural Immunology immunochromatographic assay local anaesthetic monoclonal antibody procaine strip test |
| title | Development of an immunochromatographic test strip for the detection of procaine in milk |
| title_full | Development of an immunochromatographic test strip for the detection of procaine in milk |
| title_fullStr | Development of an immunochromatographic test strip for the detection of procaine in milk |
| title_full_unstemmed | Development of an immunochromatographic test strip for the detection of procaine in milk |
| title_short | Development of an immunochromatographic test strip for the detection of procaine in milk |
| title_sort | development of an immunochromatographic test strip for the detection of procaine in milk |
| topic | immunochromatographic assay local anaesthetic monoclonal antibody procaine strip test |
| url | http://dx.doi.org/10.1080/09540105.2018.1523371 |
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